safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	28	29	40.31

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
FIAEDMOO_1	615485	616354	rfbA	Capsule	Immune modulation	100.00	100.00	WP_011681286
FIAEDMOO_1	616354	616947	STER_RS06030	Capsule	Immune modulation	100.00	100.00	WP_011226149
FIAEDMOO_1	392358	393074	STER_RS07085	Capsule	Immune modulation	100.00	100.00	WP_011681442
FIAEDMOO_1	391389	392357	STER_RS07090	Capsule	Immune modulation	100.00	100.00	WP_011681443
FIAEDMOO_1	398427	400127	STER_RS07050	Capsule	Immune modulation	99.94	100.00	WP_041827753
FIAEDMOO_1	394315	395463	STER_RS07070	Capsule	Immune modulation	99.91	100.00	WP_011681439
FIAEDMOO_1	400129	401874	STER_RS07045	Capsule	Immune modulation	99.89	100.00	WP_011681434
FIAEDMOO_1	395460	396401	STER_RS07065	Capsule	Immune modulation	99.89	100.00	WP_011681438
FIAEDMOO_1	393420	394196	STER_RS07075	Capsule	Immune modulation	99.87	100.00	WP_011681440
FIAEDMOO_1	397207	398409	STER_RS07055	Capsule	Immune modulation	99.83	100.00	WP_011681436
FIAEDMOO_1	616354	616947	STR_RS05880	Capsule	Immune modulation	99.83	100.00	WP_011226149
FIAEDMOO_1	398427	400127	STR_RS06890	Capsule	Immune modulation	99.77	100.00	WP_041827077
FIAEDMOO_1	616354	616947	STU_RS15260	Capsule	Immune modulation	99.66	100.00	WP_011226149
FIAEDMOO_1	853805	855463	fbp54	FBPs	Adherence	99.58	100.00	WP_011227179
FIAEDMOO_1	397207	398409	STR_RS06895	Capsule	Immune modulation	99.58	100.00	WP_011227428
FIAEDMOO_1	43681	44520	cbpD	Autolysin	Exoenzyme	99.52	100.00	WP_011226733
FIAEDMOO_1	396401	397207	STER_RS07060	Capsule	Immune modulation	99.50	100.00	WP_011681437
FIAEDMOO_1	390394	391371	STER_RS07095	Capsule	Immune modulation	99.39	100.00	WP_011681444
FIAEDMOO_1	395460	396401	STR_RS06905	Capsule	Immune modulation	99.36	100.00	WP_011227430
FIAEDMOO_1	394315	395463	STR_RS06910	Capsule	Immune modulation	99.30	100.00	WP_011227431
FIAEDMOO_1	579395	580156	srtA	Sortase A	Exoenzyme	99.08	100.00	-
FIAEDMOO_1	396401	397207	STR_RS06900	Capsule	Immune modulation	99.01	100.00	WP_011227429
FIAEDMOO_1	743090	743821	STER_RS05315	Capsule	Immune modulation	98.91	100.00	WP_011681176
FIAEDMOO_1	389139	390397	STER_RS07100	Capsule	Immune modulation	98.81	99.92	WP_011681445
FIAEDMOO_1	743090	743821	STU_RS14645	Capsule	Immune modulation	98.63	100.00	WP_011226048
FIAEDMOO_1	743090	743821	STR_RS05260	Capsule	Immune modulation	98.50	100.00	WP_011227248
FIAEDMOO_1	752443	753858	STER_RS05250	Capsule	Immune modulation	98.31	100.00	WP_011681165
FIAEDMOO_1	672493	673281	cshA	Cell surface hydrophobicity proteins	Adherence	98.23	99.75	-
FIAEDMOO_1	400129	401874	STR_RS06885	Capsule	Immune modulation	98.22	100.00	WP_011227426
FIAEDMOO_1	741629	743089	STER_RS05320	Capsule	Immune modulation	97.95	100.00	WP_011681177
FIAEDMOO_1	743830	744522	STR_RS05255	Capsule	Immune modulation	97.69	100.00	WP_011227247
FIAEDMOO_1	743830	744522	STU_RS14640	Capsule	Immune modulation	97.69	100.00	WP_011226047
FIAEDMOO_1	744532	745270	STER_RS05305	Capsule	Immune modulation	97.56	99.73	WP_011681174
FIAEDMOO_1	388169	389020	rfbD	Capsule	Immune modulation	97.42	100.00	WP_014621820
FIAEDMOO_1	741629	743089	STU_RS14650	Capsule	Immune modulation	97.06	100.21	WP_011226049
FIAEDMOO_1	744532	745270	STU_RS14635	Capsule	Immune modulation	96.89	99.73	WP_011226046
FIAEDMOO_1	744532	745267	STR_RS05250	Capsule	Immune modulation	96.74	98.13	WP_011227246
FIAEDMOO_1	741629	743089	STR_RS05265	Capsule	Immune modulation	96.10	100.21	WP_041827043
FIAEDMOO_1	745330	746697	STU_RS14630	Capsule	Immune modulation	94.96	100.00	WP_011226045
FIAEDMOO_1	745330	746695	STER_RS05300	Capsule	Immune modulation	94.36	99.85	WP_011681173
FIAEDMOO_1	743830	744522	STER_RS05310	Capsule	Immune modulation	89.90	100.00	WP_011681175
FIAEDMOO_1	1120633	1121936	eno	Streptococcal enolase	Exoenzyme	88.18	99.46	WP_000022832
FIAEDMOO_1	94876	95886	plr/gapA	Streptococcal plasmin receptor/GAPDH	Adherence	86.54	99.41	WP_002986042
FIAEDMOO_1	396401	397207	SGO_RS05020	Capsule	Immune modulation	83.27	100.00	WP_012000440
FIAEDMOO_1	1573866	1575149	tig/ropA	Trigger factor	Stress survival	80.73	99.77	WP_000107753
FIAEDMOO_1	617376	618422	rfbB	Capsule	Immune modulation	80.61	100.00	WP_009659423
FIAEDMOO_1	397207	397944	SGO_RS05025	Capsule	Immune modulation	84.96	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
FIAEDMOO_1	1012302	1011229	MntE	0.0	manganese homeostasis	94.97	99.72	ABF32167
FIAEDMOO_1	576708	575725	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
FIAEDMOO_1	1205655	1204687	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
FIAEDMOO_1	221886	219583	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
FIAEDMOO_1	503280	501799	SPD_0310	0.0	UPF0371 protein SPD_0310	92.31	100.00	ABJ54480
FIAEDMOO_1	976848	974602	metE	0.0	methionine biosynthesis enzymes	91.72	100.00	AAK74738
FIAEDMOO_1	72128	73126	recA	0.0	DNA recombination and repair	91.29	85.82	AAL00560
FIAEDMOO_1	27198	28169	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
FIAEDMOO_1	336892	338043	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
FIAEDMOO_1	375060	377048	uvrB	0.0	excinuclease ABC, B subunit	88.84	100.00	AE014259_2
FIAEDMOO_1	592911	594641	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	88.04	100.00	AAK34199
FIAEDMOO_1	1824643	1823165	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.42	100.00	ADE32434
FIAEDMOO_1	1121936	1120635	Eno	0.0	enolase	86.21	99.77	ABP90468
FIAEDMOO_1	1809455	1807563	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	86.05	98.90	ABP91129
FIAEDMOO_1	971677	973386	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
FIAEDMOO_1	1680212	1681222	pdh	0.0	pyruvate dehydrogenase	82.79	99.70	ABP91006
FIAEDMOO_1	826216	827577	mnmE	0.0	central tRNA-modifying GTPase	81.80	99.56	ABP90420
FIAEDMOO_1	1730571	1728679	PepO	0.0	endopeptidase	80.19	100.00	ABF32980
FIAEDMOO_1	1136371	1135481	bglB	1.44e-178	putative phospho--glucosidase	88.89	99.66	AAK33487
FIAEDMOO_1	913707	914645	SP_0676	2.33e-167	transcription facot	81.15	97.20	AAK74821
FIAEDMOO_1	974410	973553	metF	2.53e-164	methionine biosynthesis enzymes	86.01	99.31	AAK74739
FIAEDMOO_1	704796	705500	covR	4.34e-131	responder protein	85.53	100.00	AAK71319
FIAEDMOO_1	531373	532044	rr01	4.14e-125	response regulator	84.38	99.56	CAB54566
FIAEDMOO_1	704796	705500	walR	1.83e-121	DNA-binding response regulator	82.13	100.00	ANC99504
FIAEDMOO_1	1415986	1415300	CovR	9.97e-118	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
FIAEDMOO_1	967724	967056	CiaR	2.00e-117	two-component response regulator	84.31	99.55	AAK74935
FIAEDMOO_1	967724	967053	ciaR	1.97e-115	two component system response regulator	82.14	99.12	AIK75708
FIAEDMOO_1	73402	73776	SpxA2	5.28e-69	transcriptional regulator	90.40	94.70	ERL21461
FIAEDMOO_1	814857	814456	SpxA1	3.73e-66	transcriptional regulator	80.60	100.00	ERL19273
FIAEDMOO_1	1573708	1573208	RopE (M5005_Spy_1611)	4.87e-75	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
FIAEDMOO_1	221886	219589	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
FIAEDMOO_1	1716097	1715078	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
FIAEDMOO_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found