safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	28	29	40.31

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
AHHMEPMJ_1	1117365	1118234	rfbA	Capsule	Immune modulation	100.00	100.00	WP_011681286
AHHMEPMJ_1	1116772	1117365	STER_RS06030	Capsule	Immune modulation	100.00	100.00	WP_011226149
AHHMEPMJ_1	1339227	1339943	STER_RS07085	Capsule	Immune modulation	100.00	100.00	WP_011681442
AHHMEPMJ_1	1339944	1340912	STER_RS07090	Capsule	Immune modulation	100.00	100.00	WP_011681443
AHHMEPMJ_1	1332174	1333874	STER_RS07050	Capsule	Immune modulation	99.94	100.00	WP_041827753
AHHMEPMJ_1	1336838	1337986	STER_RS07070	Capsule	Immune modulation	99.91	100.00	WP_011681439
AHHMEPMJ_1	1330427	1332172	STER_RS07045	Capsule	Immune modulation	99.89	100.00	WP_011681434
AHHMEPMJ_1	1335900	1336841	STER_RS07065	Capsule	Immune modulation	99.89	100.00	WP_011681438
AHHMEPMJ_1	1338105	1338881	STER_RS07075	Capsule	Immune modulation	99.87	100.00	WP_011681440
AHHMEPMJ_1	1333892	1335094	STER_RS07055	Capsule	Immune modulation	99.83	100.00	WP_011681436
AHHMEPMJ_1	1116772	1117365	STR_RS05880	Capsule	Immune modulation	99.83	100.00	WP_011226149
AHHMEPMJ_1	1332174	1333874	STR_RS06890	Capsule	Immune modulation	99.77	100.00	WP_041827077
AHHMEPMJ_1	1116772	1117365	STU_RS15260	Capsule	Immune modulation	99.66	100.00	WP_011226149
AHHMEPMJ_1	876766	878424	fbp54	FBPs	Adherence	99.58	100.00	WP_011227179
AHHMEPMJ_1	1333892	1335094	STR_RS06895	Capsule	Immune modulation	99.58	100.00	WP_011227428
AHHMEPMJ_1	43637	44476	cbpD	Autolysin	Exoenzyme	99.52	100.00	WP_011226733
AHHMEPMJ_1	1335094	1335900	STER_RS07060	Capsule	Immune modulation	99.50	100.00	WP_011681437
AHHMEPMJ_1	1340930	1341907	STER_RS07095	Capsule	Immune modulation	99.39	100.00	WP_011681444
AHHMEPMJ_1	1335900	1336841	STR_RS06905	Capsule	Immune modulation	99.36	100.00	WP_011227430
AHHMEPMJ_1	1336838	1337986	STR_RS06910	Capsule	Immune modulation	99.30	100.00	WP_011227431
AHHMEPMJ_1	1153563	1154324	srtA	Sortase A	Exoenzyme	99.08	100.00	-
AHHMEPMJ_1	1335094	1335900	STR_RS06900	Capsule	Immune modulation	99.01	100.00	WP_011227429
AHHMEPMJ_1	989898	990629	STER_RS05315	Capsule	Immune modulation	98.91	100.00	WP_011681176
AHHMEPMJ_1	1341904	1343162	STER_RS07100	Capsule	Immune modulation	98.81	99.92	WP_011681445
AHHMEPMJ_1	989898	990629	STU_RS14645	Capsule	Immune modulation	98.63	100.00	WP_011226048
AHHMEPMJ_1	989898	990629	STR_RS05260	Capsule	Immune modulation	98.50	100.00	WP_011227248
AHHMEPMJ_1	979861	981276	STER_RS05250	Capsule	Immune modulation	98.31	100.00	WP_011681165
AHHMEPMJ_1	1060438	1061226	cshA	Cell surface hydrophobicity proteins	Adherence	98.23	99.75	-
AHHMEPMJ_1	1330427	1332172	STR_RS06885	Capsule	Immune modulation	98.22	100.00	WP_011227426
AHHMEPMJ_1	990630	992090	STER_RS05320	Capsule	Immune modulation	97.95	100.00	WP_011681177
AHHMEPMJ_1	989197	989889	STR_RS05255	Capsule	Immune modulation	97.69	100.00	WP_011227247
AHHMEPMJ_1	989197	989889	STU_RS14640	Capsule	Immune modulation	97.69	100.00	WP_011226047
AHHMEPMJ_1	988449	989187	STER_RS05305	Capsule	Immune modulation	97.56	99.73	WP_011681174
AHHMEPMJ_1	1344700	1345551	rfbD	Capsule	Immune modulation	97.42	100.00	WP_014621820
AHHMEPMJ_1	990630	992090	STU_RS14650	Capsule	Immune modulation	97.06	100.21	WP_011226049
AHHMEPMJ_1	988449	989187	STU_RS14635	Capsule	Immune modulation	96.89	99.73	WP_011226046
AHHMEPMJ_1	988452	989187	STR_RS05250	Capsule	Immune modulation	96.74	98.13	WP_011227246
AHHMEPMJ_1	990630	992090	STR_RS05265	Capsule	Immune modulation	96.10	100.21	WP_041827043
AHHMEPMJ_1	987022	988389	STU_RS14630	Capsule	Immune modulation	94.96	100.00	WP_011226045
AHHMEPMJ_1	987024	988389	STER_RS05300	Capsule	Immune modulation	94.36	99.85	WP_011681173
AHHMEPMJ_1	989197	989889	STER_RS05310	Capsule	Immune modulation	89.90	100.00	WP_011681175
AHHMEPMJ_1	612132	613435	eno	Streptococcal enolase	Exoenzyme	88.18	99.46	WP_000022832
AHHMEPMJ_1	1636323	1637333	plr/gapA	Streptococcal plasmin receptor/GAPDH	Adherence	86.54	99.41	WP_002986042
AHHMEPMJ_1	1335094	1335900	SGO_RS05020	Capsule	Immune modulation	83.27	100.00	WP_012000440
AHHMEPMJ_1	157603	158886	tig/ropA	Trigger factor	Stress survival	80.73	99.77	WP_000107753
AHHMEPMJ_1	1115297	1116343	rfbB	Capsule	Immune modulation	80.61	100.00	WP_009659423
AHHMEPMJ_1	1334357	1335094	SGO_RS05025	Capsule	Immune modulation	84.96	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
AHHMEPMJ_1	720842	721915	MntE	0.0	manganese homeostasis	94.97	99.72	ABF32167
AHHMEPMJ_1	1157011	1157994	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
AHHMEPMJ_1	527092	528060	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
AHHMEPMJ_1	1510321	1512624	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
AHHMEPMJ_1	1229021	1230502	SPD_0310	0.0	UPF0371 protein SPD_0310	92.31	100.00	ABJ54480
AHHMEPMJ_1	754153	756399	metE	0.0	methionine biosynthesis enzymes	91.72	100.00	AAK74738
AHHMEPMJ_1	72084	73082	recA	0.0	DNA recombination and repair	91.29	85.82	AAL00560
AHHMEPMJ_1	27154	28125	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
AHHMEPMJ_1	1395305	1394154	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
AHHMEPMJ_1	1358588	1356600	uvrB	0.0	excinuclease ABC, B subunit	88.84	100.00	AE014259_2
AHHMEPMJ_1	1140808	1139078	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	88.04	100.00	AAK34199
AHHMEPMJ_1	1825469	1823991	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.42	100.00	ADE32434
AHHMEPMJ_1	612132	613433	Eno	0.0	enolase	86.21	99.77	ABP90468
AHHMEPMJ_1	1810281	1808389	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	86.05	98.90	ABP91129
AHHMEPMJ_1	759324	757615	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
AHHMEPMJ_1	1679716	1680726	pdh	0.0	pyruvate dehydrogenase	82.79	99.70	ABP91006
AHHMEPMJ_1	907439	906078	mnmE	0.0	central tRNA-modifying GTPase	81.80	99.56	ABP90420
AHHMEPMJ_1	1730075	1728183	PepO	0.0	endopeptidase	80.19	100.00	ABF32980
AHHMEPMJ_1	597697	598587	bglB	1.32e-178	putative phospho--glucosidase	88.89	99.66	AAK33487
AHHMEPMJ_1	818713	817775	SP_0676	2.33e-167	transcription facot	81.15	97.20	AAK74821
AHHMEPMJ_1	756591	757448	metF	2.53e-164	methionine biosynthesis enzymes	86.01	99.31	AAK74739
AHHMEPMJ_1	1028923	1028219	covR	4.34e-131	responder protein	85.53	100.00	AAK71319
AHHMEPMJ_1	1200927	1200256	rr01	4.14e-125	response regulator	84.38	99.56	CAB54566
AHHMEPMJ_1	1028923	1028219	walR	1.83e-121	DNA-binding response regulator	82.13	100.00	ANC99504
AHHMEPMJ_1	316767	317453	CovR	9.97e-118	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
AHHMEPMJ_1	763277	763945	CiaR	2.00e-117	two-component response regulator	84.31	99.55	AAK74935
AHHMEPMJ_1	763277	763948	ciaR	1.97e-115	two component system response regulator	82.14	99.12	AIK75708
AHHMEPMJ_1	73358	73732	SpxA2	5.28e-69	transcriptional regulator	90.40	94.70	ERL21461
AHHMEPMJ_1	918798	919199	SpxA1	3.73e-66	transcriptional regulator	80.60	100.00	ERL19273
AHHMEPMJ_1	159044	159544	RopE (M5005_Spy_1611)	4.88e-75	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
AHHMEPMJ_1	1510321	1512618	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
AHHMEPMJ_1	1715601	1714582	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
AHHMEPMJ_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found