Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 24 | 28 | 36.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EKHGLMBK_1 | 43608 | 44447 | cbpD | 0.0 | Autolysin | Exoenzyme | 100.00 | 100.00 | WP_011226733 |
| EKHGLMBK_1 | 1382869 | 1384128 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 99.76 | 100.00 | WP_011681445 |
| EKHGLMBK_1 | 1380909 | 1381877 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 99.48 | 100.00 | WP_011681443 |
| EKHGLMBK_1 | 1171754 | 1172623 | rfbA | 0.0 | Capsule | Immune modulation | 99.42 | 100.00 | WP_011681286 |
| EKHGLMBK_1 | 1381895 | 1382872 | STER_RS07095 | 0.0 | Capsule | Immune modulation | 99.39 | 100.00 | WP_011681444 |
| EKHGLMBK_1 | 934356 | 936014 | fbp54 | 0.0 | FBPs | Adherence | 99.34 | 100.00 | WP_011227179 |
| EKHGLMBK_1 | 1171161 | 1171754 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | WP_011226149 |
| EKHGLMBK_1 | 1171161 | 1171754 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.16 | 100.00 | WP_011226149 |
| EKHGLMBK_1 | 1171161 | 1171754 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.16 | 100.00 | WP_011226149 |
| EKHGLMBK_1 | 1206521 | 1207282 | srtA | 0.0 | Sortase A | Exoenzyme | 99.08 | 100.00 | - |
| EKHGLMBK_1 | 1050121 | 1050852 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.63 | 100.00 | WP_011681176 |
| EKHGLMBK_1 | 1050121 | 1050852 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.36 | 100.00 | WP_011226048 |
| EKHGLMBK_1 | 1050853 | 1052307 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 98.35 | 100.00 | WP_041827043 |
| EKHGLMBK_1 | 1038693 | 1040108 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 98.31 | 100.00 | WP_011681165 |
| EKHGLMBK_1 | 1123504 | 1124292 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.23 | 99.75 | - |
| EKHGLMBK_1 | 1050121 | 1050852 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.22 | 100.00 | WP_011227248 |
| EKHGLMBK_1 | 1380192 | 1380907 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 98.18 | 99.86 | WP_011681442 |
| EKHGLMBK_1 | 1050853 | 1052307 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 98.01 | 100.00 | WP_011226049 |
| EKHGLMBK_1 | 1379070 | 1379846 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 97.81 | 100.00 | WP_011681440 |
| EKHGLMBK_1 | 1048670 | 1049410 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 97.71 | 100.00 | WP_011226046 |
| EKHGLMBK_1 | 1049420 | 1050112 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 97.69 | 100.00 | WP_011227247 |
| EKHGLMBK_1 | 1049420 | 1050112 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 97.69 | 100.00 | WP_011226047 |
| EKHGLMBK_1 | 1048670 | 1049410 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 97.57 | 100.00 | WP_011681174 |
| EKHGLMBK_1 | 1384247 | 1385098 | rfbD | 0.0 | Capsule | Immune modulation | 97.30 | 100.00 | WP_014621820 |
| EKHGLMBK_1 | 1048675 | 1049410 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 97.28 | 98.13 | WP_011227246 |
| EKHGLMBK_1 | 1050853 | 1052307 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.12 | 99.79 | WP_011681177 |
| EKHGLMBK_1 | 1047246 | 1048613 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 95.25 | 100.00 | WP_011226045 |
| EKHGLMBK_1 | 667707 | 672651 | SSU98_0978 | 0.0 | Agglutinin receptor | Adherence | 94.28 | 99.80 | YP_001200536 |
| EKHGLMBK_1 | 667707 | 672651 | SSU05_0965 | 0.0 | Agglutinin receptor | Adherence | 94.26 | 99.80 | YP_001198331 |
| EKHGLMBK_1 | 667756 | 672651 | SAG_RS09105 | 0.0 | Agglutinin receptor | Adherence | 94.18 | 100.00 | WP_000167699 |
| EKHGLMBK_1 | 1047248 | 1048613 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 93.27 | 99.85 | WP_011681173 |
| EKHGLMBK_1 | 1049420 | 1050112 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 91.05 | 100.00 | WP_011681175 |
| EKHGLMBK_1 | 601462 | 602765 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.09 | 99.54 | WP_000022832 |
| EKHGLMBK_1 | 1680281 | 1681291 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.45 | 99.90 | WP_002986042 |
| EKHGLMBK_1 | 1169685 | 1170731 | rfbB | 0.0 | Capsule | Immune modulation | 80.42 | 100.00 | WP_009659423 |
| EKHGLMBK_1 | 148583 | 149866 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.19 | 99.77 | WP_000107753 |
| EKHGLMBK_1 | 1036325 | 1036911 | SPD_RS01775 | 1.13e-141 | Capsule | Immune modulation | 82.48 | 96.70 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EKHGLMBK_1 | 667720 | 672648 | B9H01_RS04765 | 0.0 | glucan-binding protein | 94.83 | 100.00 | AUS90958 |
| EKHGLMBK_1 | 1209969 | 1210952 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| EKHGLMBK_1 | 779943 | 781016 | MntE | 0.0 | manganese homeostasis | 94.69 | 99.72 | ABF32167 |
| EKHGLMBK_1 | 1557539 | 1559842 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| EKHGLMBK_1 | 519737 | 520705 | spr0084 | 0.0 | pathogenitcity related | 92.88 | 98.48 | AAK98888 |
| EKHGLMBK_1 | 1280025 | 1281506 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.11 | 100.00 | ABJ54480 |
| EKHGLMBK_1 | 813024 | 815270 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| EKHGLMBK_1 | 64109 | 65107 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| EKHGLMBK_1 | 27125 | 28096 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| EKHGLMBK_1 | 1434169 | 1433018 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| EKHGLMBK_1 | 1398347 | 1396359 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| EKHGLMBK_1 | 1884865 | 1883387 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| EKHGLMBK_1 | 1193774 | 1192044 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.52 | 100.00 | AAK34199 |
| EKHGLMBK_1 | 1870130 | 1868238 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| EKHGLMBK_1 | 601462 | 602763 | Eno | 0.0 | enolase | 85.98 | 99.77 | ABP90468 |
| EKHGLMBK_1 | 818195 | 816486 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| EKHGLMBK_1 | 1725094 | 1726104 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| EKHGLMBK_1 | 960506 | 959145 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| EKHGLMBK_1 | 587037 | 587927 | bglB | 1.36e-178 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| EKHGLMBK_1 | 877488 | 876550 | SP_0676 | 2.41e-167 | transcription facot | 81.15 | 97.20 | AAK74821 |
| EKHGLMBK_1 | 815462 | 816319 | metF | 4.76e-164 | methionine biosynthesis enzymes | 86.01 | 99.31 | AAK74739 |
| EKHGLMBK_1 | 1089122 | 1088418 | covR | 1.64e-130 | responder protein | 85.53 | 100.00 | AAK71319 |
| EKHGLMBK_1 | 1251944 | 1251273 | rr01 | 1.52e-124 | response regulator | 83.93 | 99.56 | CAB54566 |
| EKHGLMBK_1 | 1089122 | 1088418 | walR | 8.96e-121 | DNA-binding response regulator | 81.70 | 100.00 | ANC99504 |
| EKHGLMBK_1 | 822152 | 822820 | CiaR | 9.63e-118 | two-component response regulator | 84.75 | 99.55 | AAK74935 |
| EKHGLMBK_1 | 300332 | 301018 | CovR | 1.03e-117 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| EKHGLMBK_1 | 822152 | 822823 | ciaR | 1.04e-115 | two component system response regulator | 82.59 | 99.12 | AIK75708 |
| EKHGLMBK_1 | 65383 | 65757 | SpxA2 | 2.26e-69 | transcriptional regulator | 90.40 | 94.70 | ERL21461 |
| EKHGLMBK_1 | 150024 | 150524 | RopE (M5005_Spy_1611) | 5.03e-75 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| EKHGLMBK_1 | 1557539 | 1559836 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| EKHGLMBK_1 | 1762640 | 1761621 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.18 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EKHGLMBK_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)