safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	25	29	38.29

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
HGJNPOLC_1	43685	44524	cbpD	Autolysin	Exoenzyme	100.00	100.00	WP_011226733
HGJNPOLC_1	1088906	1089775	rfbA	Capsule	Immune modulation	100.00	100.00	WP_011681286
HGJNPOLC_1	1088313	1088906	STER_RS06030	Capsule	Immune modulation	100.00	100.00	WP_011226149
HGJNPOLC_1	1292800	1293609	STU_RS16245	Capsule	Immune modulation	99.88	100.00	WP_002948020
HGJNPOLC_1	1088313	1088906	STR_RS05880	Capsule	Immune modulation	99.83	100.00	WP_011226149
HGJNPOLC_1	1293593	1294549	STU_RS16250	Capsule	Immune modulation	99.79	100.00	WP_011226328
HGJNPOLC_1	1294546	1295694	STU_RS16255	Capsule	Immune modulation	99.74	100.00	WP_041828290
HGJNPOLC_1	1088313	1088906	STU_RS15260	Capsule	Immune modulation	99.66	100.00	WP_011226149
HGJNPOLC_1	1299190	1300041	rfbD	Capsule	Immune modulation	99.65	100.00	WP_014621820
HGJNPOLC_1	857581	859239	fbp54	FBPs	Adherence	99.58	100.00	WP_011227179
HGJNPOLC_1	1291595	1292800	STU_RS16240	Capsule	Immune modulation	99.58	100.00	WP_011226326
HGJNPOLC_1	1295803	1296813	STU_RS16260	Capsule	Immune modulation	99.50	100.00	WP_011226330
HGJNPOLC_1	1288739	1290523	STU_RS16230	Capsule	Immune modulation	99.44	100.00	WP_011226325
HGJNPOLC_1	1290520	1291575	STU_RS16235	Capsule	Immune modulation	99.43	100.00	WP_002948022
HGJNPOLC_1	1125121	1125882	srtA	Sortase A	Exoenzyme	99.21	100.00	-
HGJNPOLC_1	953108	954523	STER_RS05250	Capsule	Immune modulation	98.52	100.00	WP_011681165
HGJNPOLC_1	965514	966245	STU_RS14645	Capsule	Immune modulation	98.36	100.00	WP_011226048
HGJNPOLC_1	1032195	1032983	cshA	Cell surface hydrophobicity proteins	Adherence	98.36	99.75	-
HGJNPOLC_1	965514	966245	STER_RS05315	Capsule	Immune modulation	98.09	100.00	WP_011681176
HGJNPOLC_1	965514	966245	STR_RS05260	Capsule	Immune modulation	97.68	100.00	WP_011227248
HGJNPOLC_1	966246	967706	STER_RS05320	Capsule	Immune modulation	97.54	100.00	WP_011681177
HGJNPOLC_1	1296816	1298087	STU_RS16265	Capsule	Immune modulation	97.48	99.76	WP_011226331
HGJNPOLC_1	964063	964803	STU_RS14635	Capsule	Immune modulation	97.30	100.00	WP_011226046
HGJNPOLC_1	964063	964803	STER_RS05305	Capsule	Immune modulation	96.90	100.00	WP_011681174
HGJNPOLC_1	964068	964803	STR_RS05250	Capsule	Immune modulation	96.33	98.13	WP_011227246
HGJNPOLC_1	966246	967706	STU_RS14650	Capsule	Immune modulation	96.23	100.21	WP_011226049
HGJNPOLC_1	962639	964006	STU_RS14630	Capsule	Immune modulation	95.47	100.00	WP_011226045
HGJNPOLC_1	966246	967706	STR_RS05265	Capsule	Immune modulation	95.41	100.21	WP_041827043
HGJNPOLC_1	964813	965505	STR_RS05255	Capsule	Immune modulation	95.40	99.86	WP_011227247
HGJNPOLC_1	964813	965505	STU_RS14640	Capsule	Immune modulation	95.40	99.86	WP_011226047
HGJNPOLC_1	962641	964006	STER_RS05300	Capsule	Immune modulation	94.22	99.85	WP_011681173
HGJNPOLC_1	964813	965505	STER_RS05310	Capsule	Immune modulation	89.47	100.00	WP_011681175
HGJNPOLC_1	609826	611129	eno	Streptococcal enolase	Exoenzyme	88.09	99.54	WP_000022832
HGJNPOLC_1	1589679	1590689	plr/gapA	Streptococcal plasmin receptor/GAPDH	Adherence	86.44	99.41	WP_002986042
HGJNPOLC_1	149172	150455	tig/ropA	Trigger factor	Stress survival	80.73	99.77	WP_000107753
HGJNPOLC_1	1086838	1087884	rfbB	Capsule	Immune modulation	80.61	100.00	WP_009659423

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
HGJNPOLC_1	1128569	1129552	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
HGJNPOLC_1	717562	718635	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
HGJNPOLC_1	1466712	1469015	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
HGJNPOLC_1	522893	523861	spr0084	0.0	pathogenitcity related	92.57	98.48	AAK98888
HGJNPOLC_1	739505	741751	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
HGJNPOLC_1	1198465	1199946	SPD_0310	0.0	UPF0371 protein SPD_0310	91.50	100.00	ABJ54480
HGJNPOLC_1	63314	64306	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
HGJNPOLC_1	27203	28174	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
HGJNPOLC_1	1349999	1348848	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
HGJNPOLC_1	1315031	1313043	uvrB	0.0	excinuclease ABC, B subunit	88.84	100.00	AE014259_2
HGJNPOLC_1	1112348	1110618	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.87	100.00	AAK34199
HGJNPOLC_1	1783714	1782236	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.42	100.00	ADE32434
HGJNPOLC_1	1768525	1766633	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	86.05	98.90	ABP91129
HGJNPOLC_1	609826	611127	Eno	0.0	enolase	85.98	99.77	ABP90468
HGJNPOLC_1	744676	742967	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
HGJNPOLC_1	1632690	1633700	pdh	0.0	pyruvate dehydrogenase	82.79	99.70	ABP91006
HGJNPOLC_1	885728	884367	mnmE	0.0	central tRNA-modifying GTPase	81.80	99.56	ABP90420
HGJNPOLC_1	1687557	1685665	PepO	0.0	endopeptidase	80.19	100.00	ABF32980
HGJNPOLC_1	595399	596289	bglB	3.73e-178	putative phospho--glucosidase	88.55	99.66	AAK33487
HGJNPOLC_1	802641	801706	SP_0676	7.15e-168	transcription facot	81.73	96.89	AAK74821
HGJNPOLC_1	741943	742800	metF	4.50e-164	methionine biosynthesis enzymes	86.01	99.31	AAK74739
HGJNPOLC_1	1004510	1003806	covR	4.24e-131	responder protein	85.53	100.00	AAK71319
HGJNPOLC_1	1179497	1178826	rr01	5.40e-123	response regulator	83.04	99.56	CAB54566
HGJNPOLC_1	1004510	1003806	walR	1.79e-121	DNA-binding response regulator	82.13	100.00	ANC99504
HGJNPOLC_1	748630	749298	CiaR	9.11e-118	two-component response regulator	84.75	99.55	AAK74935
HGJNPOLC_1	307291	307977	CovR	9.93e-118	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
HGJNPOLC_1	748630	749301	ciaR	9.88e-116	two component system response regulator	82.59	99.12	AIK75708
HGJNPOLC_1	64603	64998	SpxA2	3.59e-74	transcriptional regulator	90.91	100.00	ERL21461
HGJNPOLC_1	897087	897488	SpxA1	3.64e-66	transcriptional regulator	80.60	100.00	ERL19273
HGJNPOLC_1	150613	151113	RopE (M5005_Spy_1611)	4.77e-75	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
HGJNPOLC_1	1466712	1469009	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
HGJNPOLC_1	1673082	1672063	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
HGJNPOLC_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found