safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	26	28	38.21

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
IJHPJAAA_1	44999	45838	cbpD	0.0	Autolysin	Exoenzyme	100.00	100.00	WP_011226733
IJHPJAAA_1	1142393	1143262	rfbA	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681286
IJHPJAAA_1	1141800	1142393	STER_RS06030	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
IJHPJAAA_1	1326315	1327124	STU_RS16245	0.0	Capsule	Immune modulation	99.88	100.00	WP_002948020
IJHPJAAA_1	1141800	1142393	STR_RS05880	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
IJHPJAAA_1	1141800	1142393	STU_RS15260	0.0	Capsule	Immune modulation	99.66	100.00	WP_011226149
IJHPJAAA_1	1322254	1324038	STU_RS16230	0.0	Capsule	Immune modulation	99.50	100.00	WP_011226325
IJHPJAAA_1	1327108	1328064	STU_RS16250	0.0	Capsule	Immune modulation	99.48	100.00	WP_011226328
IJHPJAAA_1	901882	903540	fbp54	0.0	FBPs	Adherence	99.46	100.00	WP_011227179
IJHPJAAA_1	1325110	1326315	STU_RS16240	0.0	Capsule	Immune modulation	99.25	100.00	WP_011226326
IJHPJAAA_1	1178587	1179348	srtA	0.0	Sortase A	Exoenzyme	99.08	100.00	-
IJHPJAAA_1	1324035	1325090	STU_RS16235	0.0	Capsule	Immune modulation	98.96	100.00	WP_002948022
IJHPJAAA_1	1330905	1331867	STU_RS16260	0.0	Capsule	Immune modulation	98.75	95.25	WP_011226330
IJHPJAAA_1	1013610	1014341	STER_RS05315	0.0	Capsule	Immune modulation	98.63	100.00	WP_011681176
IJHPJAAA_1	1334244	1335095	rfbD	0.0	Capsule	Immune modulation	98.59	100.00	WP_014621820
IJHPJAAA_1	1328061	1329209	STU_RS16255	0.0	Capsule	Immune modulation	98.52	100.00	WP_041828290
IJHPJAAA_1	1013610	1014341	STU_RS14645	0.0	Capsule	Immune modulation	98.36	100.00	WP_011226048
IJHPJAAA_1	1014342	1015796	STR_RS05265	0.0	Capsule	Immune modulation	98.35	100.00	WP_041827043
IJHPJAAA_1	1002182	1003597	STER_RS05250	0.0	Capsule	Immune modulation	98.31	100.00	WP_011681165
IJHPJAAA_1	1084157	1084945	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	98.23	99.75	-
IJHPJAAA_1	1013610	1014341	STR_RS05260	0.0	Capsule	Immune modulation	98.22	100.00	WP_011227248
IJHPJAAA_1	1014342	1015796	STU_RS14650	0.0	Capsule	Immune modulation	98.01	100.00	WP_011226049
IJHPJAAA_1	1012159	1012899	STU_RS14635	0.0	Capsule	Immune modulation	97.84	100.00	WP_011226046
IJHPJAAA_1	1012909	1013601	STR_RS05255	0.0	Capsule	Immune modulation	97.83	100.00	WP_011227247
IJHPJAAA_1	1012909	1013601	STU_RS14640	0.0	Capsule	Immune modulation	97.83	100.00	WP_011226047
IJHPJAAA_1	1331870	1333141	STU_RS16265	0.0	Capsule	Immune modulation	97.72	99.76	WP_011226331
IJHPJAAA_1	1012159	1012899	STER_RS05305	0.0	Capsule	Immune modulation	97.71	100.00	WP_011681174
IJHPJAAA_1	1012164	1012899	STR_RS05250	0.0	Capsule	Immune modulation	97.42	98.13	WP_011227246
IJHPJAAA_1	1014342	1015796	STER_RS05320	0.0	Capsule	Immune modulation	97.12	99.79	WP_011681177
IJHPJAAA_1	1010735	1012101	STU_RS14630	0.0	Capsule	Immune modulation	95.25	99.93	WP_011226045
IJHPJAAA_1	1010737	1012101	STER_RS05300	0.0	Capsule	Immune modulation	93.26	99.78	WP_011681173
IJHPJAAA_1	1012909	1013601	STER_RS05310	0.0	Capsule	Immune modulation	91.20	100.00	WP_011681175
IJHPJAAA_1	634786	636089	eno	0.0	Streptococcal enolase	Exoenzyme	88.10	99.46	WP_000022832
IJHPJAAA_1	1618587	1619597	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.54	99.41	WP_002986042
IJHPJAAA_1	150836	152119	tig/ropA	0.0	Trigger factor	Stress survival	80.73	99.77	WP_000107753
IJHPJAAA_1	1140325	1141371	rfbB	0.0	Capsule	Immune modulation	80.61	100.00	WP_009659423
IJHPJAAA_1	999814	1000400	SPD_RS01775	1.09e-141	Capsule	Immune modulation	82.48	96.70	WP_001819836

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
IJHPJAAA_1	1182035	1183018	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
IJHPJAAA_1	746008	747081	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
IJHPJAAA_1	549675	550643	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
IJHPJAAA_1	1495403	1497706	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
IJHPJAAA_1	777363	779609	metE	0.0	methionine biosynthesis enzymes	91.86	100.00	AAK74738
IJHPJAAA_1	65759	66757	recA	0.0	DNA recombination and repair	91.29	85.82	AAL00560
IJHPJAAA_1	28515	29486	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
IJHPJAAA_1	1379124	1377973	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
IJHPJAAA_1	1351484	1349496	uvrB	0.0	excinuclease ABC, B subunit	88.84	100.00	AE014259_2
IJHPJAAA_1	1165836	1164106	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	88.04	100.00	AAK34199
IJHPJAAA_1	1814830	1813352	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.42	100.00	ADE32434
IJHPJAAA_1	634786	636087	Eno	0.0	enolase	86.21	99.77	ABP90468
IJHPJAAA_1	1798216	1796324	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	86.05	98.90	ABP91129
IJHPJAAA_1	782534	780825	PGM	0.0	phosphoglucomutase	83.71	99.65	ACH87041
IJHPJAAA_1	1661982	1662992	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
IJHPJAAA_1	929953	928592	mnmE	0.0	central tRNA-modifying GTPase	81.80	99.56	ABP90420
IJHPJAAA_1	1716491	1714599	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
IJHPJAAA_1	620351	621241	bglB	1.31e-178	putative phospho--glucosidase	88.89	99.66	AAK33487
IJHPJAAA_1	841817	840879	SP_0676	2.32e-167	transcription facot	81.15	97.20	AAK74821
IJHPJAAA_1	779801	780658	metF	8.78e-166	methionine biosynthesis enzymes	86.71	99.31	AAK74739
IJHPJAAA_1	1052639	1051935	covR	4.31e-131	responder protein	85.53	100.00	AAK71319
IJHPJAAA_1	1229252	1228581	rr01	2.35e-123	response regulator	83.48	99.56	CAB54566
IJHPJAAA_1	1052639	1051935	walR	1.82e-121	DNA-binding response regulator	82.13	100.00	ANC99504
IJHPJAAA_1	310097	310783	CovR	9.91e-118	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
IJHPJAAA_1	786488	787156	CiaR	1.99e-117	two-component response regulator	84.31	99.55	AAK74935
IJHPJAAA_1	786488	787159	ciaR	1.96e-115	two component system response regulator	82.14	99.12	AIK75708
IJHPJAAA_1	67033	67407	SpxA2	1.14e-69	transcriptional regulator	91.20	94.70	ERL21461
IJHPJAAA_1	941314	941715	SpxA1	3.71e-66	transcriptional regulator	80.60	100.00	ERL19273
IJHPJAAA_1	152277	152777	RopE (M5005_Spy_1611)	4.85e-75	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
IJHPJAAA_1	1495403	1497700	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
IJHPJAAA_1	1702022	1701003	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
IJHPJAAA_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found