safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	25	29	38.29

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
OONJGCHM_1	43701	44540	cbpD	Autolysin	Exoenzyme	100.00	100.00	WP_011226733
OONJGCHM_1	1088937	1089806	rfbA	Capsule	Immune modulation	100.00	100.00	WP_011681286
OONJGCHM_1	1088344	1088937	STER_RS06030	Capsule	Immune modulation	100.00	100.00	WP_011226149
OONJGCHM_1	1292827	1293636	STU_RS16245	Capsule	Immune modulation	99.88	100.00	WP_002948020
OONJGCHM_1	1088344	1088937	STR_RS05880	Capsule	Immune modulation	99.83	100.00	WP_011226149
OONJGCHM_1	1293620	1294576	STU_RS16250	Capsule	Immune modulation	99.79	100.00	WP_011226328
OONJGCHM_1	1294573	1295721	STU_RS16255	Capsule	Immune modulation	99.74	100.00	WP_041828290
OONJGCHM_1	1088344	1088937	STU_RS15260	Capsule	Immune modulation	99.66	100.00	WP_011226149
OONJGCHM_1	1299217	1300068	rfbD	Capsule	Immune modulation	99.65	100.00	WP_014621820
OONJGCHM_1	857613	859271	fbp54	FBPs	Adherence	99.58	100.00	WP_011227179
OONJGCHM_1	1291622	1292827	STU_RS16240	Capsule	Immune modulation	99.58	100.00	WP_011226326
OONJGCHM_1	1295830	1296840	STU_RS16260	Capsule	Immune modulation	99.50	100.00	WP_011226330
OONJGCHM_1	1288766	1290550	STU_RS16230	Capsule	Immune modulation	99.44	100.00	WP_011226325
OONJGCHM_1	1290547	1291602	STU_RS16235	Capsule	Immune modulation	99.43	100.00	WP_002948022
OONJGCHM_1	1125152	1125913	srtA	Sortase A	Exoenzyme	99.21	100.00	-
OONJGCHM_1	953139	954554	STER_RS05250	Capsule	Immune modulation	98.52	100.00	WP_011681165
OONJGCHM_1	965545	966276	STU_RS14645	Capsule	Immune modulation	98.36	100.00	WP_011226048
OONJGCHM_1	1032226	1033014	cshA	Cell surface hydrophobicity proteins	Adherence	98.36	99.75	-
OONJGCHM_1	965545	966276	STER_RS05315	Capsule	Immune modulation	98.09	100.00	WP_011681176
OONJGCHM_1	965545	966276	STR_RS05260	Capsule	Immune modulation	97.68	100.00	WP_011227248
OONJGCHM_1	966277	967737	STER_RS05320	Capsule	Immune modulation	97.54	100.00	WP_011681177
OONJGCHM_1	1296843	1298114	STU_RS16265	Capsule	Immune modulation	97.48	99.76	WP_011226331
OONJGCHM_1	964094	964834	STU_RS14635	Capsule	Immune modulation	97.30	100.00	WP_011226046
OONJGCHM_1	964094	964834	STER_RS05305	Capsule	Immune modulation	96.90	100.00	WP_011681174
OONJGCHM_1	964099	964834	STR_RS05250	Capsule	Immune modulation	96.33	98.13	WP_011227246
OONJGCHM_1	966277	967737	STU_RS14650	Capsule	Immune modulation	96.23	100.21	WP_011226049
OONJGCHM_1	962670	964037	STU_RS14630	Capsule	Immune modulation	95.47	100.00	WP_011226045
OONJGCHM_1	966277	967737	STR_RS05265	Capsule	Immune modulation	95.41	100.21	WP_041827043
OONJGCHM_1	964844	965536	STR_RS05255	Capsule	Immune modulation	95.40	99.86	WP_011227247
OONJGCHM_1	964844	965536	STU_RS14640	Capsule	Immune modulation	95.40	99.86	WP_011226047
OONJGCHM_1	962672	964037	STER_RS05300	Capsule	Immune modulation	94.22	99.85	WP_011681173
OONJGCHM_1	964844	965536	STER_RS05310	Capsule	Immune modulation	89.47	100.00	WP_011681175
OONJGCHM_1	609865	611168	eno	Streptococcal enolase	Exoenzyme	88.09	99.54	WP_000022832
OONJGCHM_1	1589703	1590713	plr/gapA	Streptococcal plasmin receptor/GAPDH	Adherence	86.44	99.41	WP_002986042
OONJGCHM_1	149209	150492	tig/ropA	Trigger factor	Stress survival	80.73	99.77	WP_000107753
OONJGCHM_1	1086869	1087915	rfbB	Capsule	Immune modulation	80.61	100.00	WP_009659423

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
OONJGCHM_1	1128600	1129583	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
OONJGCHM_1	717601	718674	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
OONJGCHM_1	1466737	1469040	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
OONJGCHM_1	522932	523900	spr0084	0.0	pathogenitcity related	92.57	98.48	AAK98888
OONJGCHM_1	739544	741790	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
OONJGCHM_1	1198496	1199977	SPD_0310	0.0	UPF0371 protein SPD_0310	91.50	100.00	ABJ54480
OONJGCHM_1	63330	64322	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
OONJGCHM_1	27219	28190	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
OONJGCHM_1	1350026	1348875	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
OONJGCHM_1	1315058	1313070	uvrB	0.0	excinuclease ABC, B subunit	88.84	100.00	AE014259_2
OONJGCHM_1	1112379	1110649	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.87	100.00	AAK34199
OONJGCHM_1	1783739	1782261	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.42	100.00	ADE32434
OONJGCHM_1	1768550	1766658	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	86.05	98.90	ABP91129
OONJGCHM_1	609865	611166	Eno	0.0	enolase	85.98	99.77	ABP90468
OONJGCHM_1	744715	743006	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
OONJGCHM_1	1632713	1633723	pdh	0.0	pyruvate dehydrogenase	82.79	99.70	ABP91006
OONJGCHM_1	885760	884399	mnmE	0.0	central tRNA-modifying GTPase	81.80	99.56	ABP90420
OONJGCHM_1	1687582	1685690	PepO	0.0	endopeptidase	80.19	100.00	ABF32980
OONJGCHM_1	595438	596328	bglB	3.73e-178	putative phospho--glucosidase	88.55	99.66	AAK33487
OONJGCHM_1	802673	801738	SP_0676	7.15e-168	transcription facot	81.73	96.89	AAK74821
OONJGCHM_1	741982	742839	metF	4.50e-164	methionine biosynthesis enzymes	86.01	99.31	AAK74739
OONJGCHM_1	1004541	1003837	covR	4.24e-131	responder protein	85.53	100.00	AAK71319
OONJGCHM_1	1179528	1178857	rr01	5.40e-123	response regulator	83.04	99.56	CAB54566
OONJGCHM_1	1004541	1003837	walR	1.79e-121	DNA-binding response regulator	82.13	100.00	ANC99504
OONJGCHM_1	748669	749337	CiaR	9.11e-118	two-component response regulator	84.75	99.55	AAK74935
OONJGCHM_1	307327	308013	CovR	9.93e-118	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
OONJGCHM_1	748669	749340	ciaR	9.88e-116	two component system response regulator	82.59	99.12	AIK75708
OONJGCHM_1	64619	65014	SpxA2	3.59e-74	transcriptional regulator	90.91	100.00	ERL21461
OONJGCHM_1	897119	897520	SpxA1	3.64e-66	transcriptional regulator	80.60	100.00	ERL19273
OONJGCHM_1	150650	151150	RopE (M5005_Spy_1611)	4.77e-75	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
OONJGCHM_1	1466737	1469034	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
OONJGCHM_1	1673107	1672088	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
OONJGCHM_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found