Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 27 | 31 | 41.11 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| PNCBHKKH_1 | 1184274 | 1185143 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| PNCBHKKH_1 | 1183681 | 1184274 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| PNCBHKKH_1 | 1417102 | 1417911 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.88 | 100.00 | WP_002948020 |
| PNCBHKKH_1 | 1183681 | 1184274 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| PNCBHKKH_1 | 1417895 | 1418851 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.79 | 100.00 | WP_011226328 |
| PNCBHKKH_1 | 1418848 | 1419996 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | WP_041828290 |
| PNCBHKKH_1 | 1183681 | 1184274 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| PNCBHKKH_1 | 1423492 | 1424343 | rfbD | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | WP_014621820 |
| PNCBHKKH_1 | 942896 | 944554 | fbp54 | 0.0 | FBPs | Adherence | 99.58 | 100.00 | WP_011227179 |
| PNCBHKKH_1 | 1415897 | 1417102 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | WP_011226326 |
| PNCBHKKH_1 | 43680 | 44519 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.52 | 100.00 | WP_011226733 |
| PNCBHKKH_1 | 1057119 | 1057718 | STR_RS05245 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_196767671 |
| PNCBHKKH_1 | 1420105 | 1421115 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226330 |
| PNCBHKKH_1 | 1413041 | 1414825 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.44 | 100.00 | WP_011226325 |
| PNCBHKKH_1 | 1414822 | 1415877 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.43 | 100.00 | WP_002948022 |
| PNCBHKKH_1 | 1036419 | 1037834 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | WP_011681165 |
| PNCBHKKH_1 | 1057870 | 1058605 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 98.91 | 99.33 | WP_011681174 |
| PNCBHKKH_1 | 1220468 | 1221229 | srtA | 0.0 | Sortase A | Exoenzyme | 98.82 | 100.00 | - |
| PNCBHKKH_1 | 1059316 | 1060047 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.77 | 100.00 | WP_011681176 |
| PNCBHKKH_1 | 1060048 | 1061502 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 98.76 | 100.00 | WP_041827043 |
| PNCBHKKH_1 | 1055995 | 1056954 | STR_RS05240 | 0.0 | Capsule | Immune modulation | 98.54 | 100.00 | WP_041827042 |
| PNCBHKKH_1 | 1059316 | 1060047 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.50 | 100.00 | WP_011226048 |
| PNCBHKKH_1 | 1126026 | 1126819 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.36 | 100.25 | - |
| PNCBHKKH_1 | 1059316 | 1060047 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.36 | 100.00 | WP_011227248 |
| PNCBHKKH_1 | 1057856 | 1058605 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 98.27 | 100.00 | WP_011227246 |
| PNCBHKKH_1 | 1060048 | 1061502 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 98.21 | 100.00 | WP_011226049 |
| PNCBHKKH_1 | 1057870 | 1058605 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 98.10 | 99.33 | WP_011226046 |
| PNCBHKKH_1 | 1421118 | 1422389 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 97.48 | 99.76 | WP_011226331 |
| PNCBHKKH_1 | 1060048 | 1061502 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 96.65 | 99.79 | WP_011681177 |
| PNCBHKKH_1 | 539613 | 544509 | SAG_RS09105 | 0.0 | Agglutinin receptor | Adherence | 92.79 | 99.96 | WP_000167699 |
| PNCBHKKH_1 | 539565 | 544509 | SSU98_0978 | 0.0 | Agglutinin receptor | Adherence | 92.77 | 99.78 | YP_001200536 |
| PNCBHKKH_1 | 539565 | 544509 | SSU05_0965 | 0.0 | Agglutinin receptor | Adherence | 92.75 | 99.78 | YP_001198331 |
| PNCBHKKH_1 | 1058615 | 1059307 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.90 | 100.00 | WP_011681175 |
| PNCBHKKH_1 | 1058615 | 1059307 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 89.75 | 100.00 | WP_011226047 |
| PNCBHKKH_1 | 1058615 | 1059307 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 89.47 | 100.00 | WP_011227247 |
| PNCBHKKH_1 | 686572 | 687875 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| PNCBHKKH_1 | 1715319 | 1716329 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.44 | 99.41 | WP_002986042 |
| PNCBHKKH_1 | 152002 | 153285 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.73 | 99.77 | WP_000107753 |
| PNCBHKKH_1 | 1182206 | 1183252 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| PNCBHKKH_1 | 1223916 | 1224899 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| PNCBHKKH_1 | 796766 | 797839 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| PNCBHKKH_1 | 602873 | 603841 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| PNCBHKKH_1 | 1592158 | 1594461 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| PNCBHKKH_1 | 539735 | 544504 | B9H01_RS04765 | 0.0 | glucan-binding protein | 92.77 | 96.77 | AUS90958 |
| PNCBHKKH_1 | 1313280 | 1314761 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.31 | 100.00 | ABJ54480 |
| PNCBHKKH_1 | 818708 | 820954 | metE | 0.0 | methionine biosynthesis enzymes | 91.72 | 100.00 | AAK74738 |
| PNCBHKKH_1 | 72289 | 73287 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| PNCBHKKH_1 | 27197 | 28168 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| PNCBHKKH_1 | 1477130 | 1475979 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| PNCBHKKH_1 | 1439313 | 1437325 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| PNCBHKKH_1 | 1207717 | 1205987 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| PNCBHKKH_1 | 1923621 | 1922143 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.22 | 100.00 | ADE32434 |
| PNCBHKKH_1 | 686572 | 687873 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| PNCBHKKH_1 | 1908432 | 1906540 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| PNCBHKKH_1 | 823879 | 822170 | PGM | 0.0 | phosphoglucomutase | 83.71 | 99.65 | ACH87041 |
| PNCBHKKH_1 | 1757810 | 1758820 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| PNCBHKKH_1 | 970968 | 969607 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| PNCBHKKH_1 | 1809854 | 1807962 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| PNCBHKKH_1 | 672137 | 673027 | bglB | 1.39e-178 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| PNCBHKKH_1 | 884932 | 883994 | SP_0676 | 2.46e-167 | transcription facot | 81.15 | 97.20 | AAK74821 |
| PNCBHKKH_1 | 821146 | 822003 | metF | 1.60e-164 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| PNCBHKKH_1 | 1098345 | 1097641 | covR | 4.57e-131 | responder protein | 85.53 | 100.00 | AAK71319 |
| PNCBHKKH_1 | 1272088 | 1271417 | rr01 | 3.14e-124 | response regulator | 83.93 | 99.56 | CAB54566 |
| PNCBHKKH_1 | 1098345 | 1097641 | walR | 1.93e-121 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| PNCBHKKH_1 | 310042 | 310728 | CovR | 1.05e-117 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| PNCBHKKH_1 | 827833 | 828501 | CiaR | 2.11e-117 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| PNCBHKKH_1 | 827833 | 828504 | ciaR | 2.08e-115 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| PNCBHKKH_1 | 73563 | 73937 | SpxA2 | 1.20e-69 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| PNCBHKKH_1 | 982327 | 982728 | SpxA1 | 3.93e-66 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| PNCBHKKH_1 | 153443 | 153943 | RopE (M5005_Spy_1611) | 5.14e-75 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| PNCBHKKH_1 | 1592158 | 1594455 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| PNCBHKKH_1 | 574217 | 575413 | AUS90997 (B9H01_RS07385) | 0.0 | site-specific integrase | 97.74 | 100.00 | AUS90997 |
| PNCBHKKH_1 | 1795382 | 1794363 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| PNCBHKKH_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)