safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	9	18.71

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
DHBPGPAN_1	678257	679426	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
DHBPGPAN_1	455906	457108	blt	ARO:3003006	blt	100.00	100.00	L32599.1
DHBPGPAN_1	437545	438399	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
DHBPGPAN_4	279919	280839	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1
DHBPGPAN_6	276605	279175	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
DHBPGPAN_4	266585	268024	lmrB	ARO:3002813	lmrB	99.79	100.42	JYFL01000006.1
DHBPGPAN_2	259241	259558	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
DHBPGPAN_2	258903	259241	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
DHBPGPAN_4	216928	217521	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
DHBPGPAN_9	47432	49075	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
DHBPGPAN_1	437545	438399	aadK	Streptomycin	100.00	100.00	M26879
DHBPGPAN_4	279919	280839	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
DHBPGPAN_3	36437	37813	tet(L)	Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
DHBPGPAN_1	437545	438396	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
DHBPGPAN_4	279922	280839	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
DHBPGPAN_3	39813	40331	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
DHBPGPAN_3	36437	37810	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
DHBPGPAN_9	47435	49075	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
DHBPGPAN_7	192872	195466	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
DHBPGPAN_3	525338	526480	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
DHBPGPAN_3	523674	524855	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
DHBPGPAN_3	524870	525319	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
DHBPGPAN_7	240361	242124	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
DHBPGPAN_5	212843	213628	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
DHBPGPAN_5	216526	217464	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
DHBPGPAN_5	213654	214850	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
DHBPGPAN_5	214879	216498	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
DHBPGPAN_5	217484	224620	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
DHBPGPAN_1	878140	878781	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
DHBPGPAN_10	28088	26952	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
DHBPGPAN_12	4342	5211	pdxS	1.12e-151	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
DHBPGPAN_2	572438	573214	CodY	6.89e-125	nutrient-responsive regulator	81.08	100.00	ACJ80679
DHBPGPAN_3	678067	677498	ClpP	4.34e-99	part of proteolytic complex	81.05	97.44	BAB94595
DHBPGPAN_2	110079	110471	spxA1	2.04e-69	redox-responsive transcription factor	83.21	100.00	AAF21893
DHBPGPAN_11	20849	21091	SpoVG	2.74e-38	RNA-binding protein	80.25	79.41	AAT02994
DHBPGPAN_9	1960	2157	CspA	3.38e-32	cold shock protein	90.91	100.00	CAA62903
DHBPGPAN_1	866178	865984	CspD	5.70e-28	cold shock protein	81.54	98.48	CAC99957
DHBPGPAN_8	132531	132725	CspB	1.52e-27	cold shock protein	83.08	98.48	CAD00094

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
DHBPGPAN_1	0.0	1.0	0.0
DHBPGPAN_2	0.0	1.0	0.0
DHBPGPAN_3	0.0	1.0	0.0
DHBPGPAN_4	0.0	1.0	0.0
DHBPGPAN_5	0.0	1.0	0.0
DHBPGPAN_6	0.0	1.0	0.0
DHBPGPAN_7	0.0	1.0	0.0
DHBPGPAN_8	0.0	1.0	0.0
DHBPGPAN_9	0.0	1.0	0.0
DHBPGPAN_10	0.0	1.0	0.0
DHBPGPAN_11	0.0	1.0	0.0
DHBPGPAN_12	0.0	1.0	0.0
DHBPGPAN_13	0.0	1.0	0.0
DHBPGPAN_14	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
DHBPGPAN_1	476659	507036	False	Myoviridae	VOG4600, VOG1309, VOG11060, VOG0189, VOG4693, VOG9587, VOG4673, VOG0796, VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718
DHBPGPAN_2	202956	229064	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705

Phigaro v2.4.0 (Database: Jan. 2024 release)