Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 23 | 34 | 41.05 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KGGNJBNG_23 | 6808 | 8268 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.06 | 100.00 | WP_011681177 |
| KGGNJBNG_23 | 6808 | 8268 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.72 | 100.21 | WP_011226049 |
| KGGNJBNG_23 | 6808 | 8268 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.17 | 100.21 | WP_041827043 |
| KGGNJBNG_26 | 69368 | 70177 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 95.56 | 100.00 | WP_002948020 |
| KGGNJBNG_23 | 10508 | 11873 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 95.09 | 99.85 | WP_011681173 |
| KGGNJBNG_26 | 77302 | 78153 | rfbD | 0.0 | Capsule | Immune modulation | 95.07 | 100.00 | WP_014621820 |
| KGGNJBNG_23 | 9711 | 10451 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 94.74 | 100.00 | WP_011681174 |
| KGGNJBNG_23 | 10508 | 11875 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.59 | 100.00 | WP_011226045 |
| KGGNJBNG_23 | 9711 | 10451 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 94.33 | 100.00 | WP_011226046 |
| KGGNJBNG_23 | 9711 | 10446 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 93.75 | 98.13 | WP_011227246 |
| KGGNJBNG_26 | 74930 | 76201 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 93.63 | 99.76 | WP_011226331 |
| KGGNJBNG_26 | 70161 | 71117 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 93.52 | 100.00 | WP_011226328 |
| KGGNJBNG_26 | 65328 | 67112 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 92.66 | 100.00 | WP_011226325 |
| KGGNJBNG_26 | 68163 | 69368 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 92.37 | 100.00 | WP_011226326 |
| KGGNJBNG_23 | 8269 | 9000 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 92.08 | 100.00 | WP_011226048 |
| KGGNJBNG_26 | 71114 | 72262 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 91.99 | 100.00 | WP_041828290 |
| KGGNJBNG_26 | 67109 | 68143 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 91.77 | 99.05 | WP_002948022 |
| KGGNJBNG_23 | 8269 | 9000 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 91.67 | 100.00 | WP_011681176 |
| KGGNJBNG_23 | 8269 | 9000 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 91.12 | 100.00 | WP_011227248 |
| KGGNJBNG_23 | 115653 | 117311 | fbp54 | 0.0 | FBPs | Adherence | 91.08 | 99.94 | WP_011227179 |
| KGGNJBNG_1 | 83197 | 84066 | rfbA | 0.0 | Capsule | Immune modulation | 91.03 | 100.00 | WP_011681286 |
| KGGNJBNG_1 | 82604 | 83197 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| KGGNJBNG_1 | 82604 | 83197 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 90.74 | 100.00 | WP_011226149 |
| KGGNJBNG_1 | 82604 | 83197 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 90.74 | 100.00 | WP_011226149 |
| KGGNJBNG_23 | 9009 | 9701 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 88.92 | 99.71 | WP_011227247 |
| KGGNJBNG_23 | 9009 | 9701 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 88.92 | 99.71 | WP_011226047 |
| KGGNJBNG_1 | 248980 | 250283 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.24 | 99.54 | WP_000022832 |
| KGGNJBNG_12 | 14314 | 15324 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.66 | 99.80 | WP_002986042 |
| KGGNJBNG_25 | 97224 | 98114 | cbpD | 0.0 | Autolysin | Exoenzyme | 87.64 | 93.29 | WP_082302295 |
| KGGNJBNG_23 | 9009 | 9701 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 87.05 | 99.71 | WP_011681175 |
| KGGNJBNG_27 | 31027 | 31788 | srtA | 0.0 | Sortase A | Exoenzyme | 84.40 | 99.87 | - |
| KGGNJBNG_1 | 81286 | 82332 | rfbB | 0.0 | Capsule | Immune modulation | 83.97 | 99.90 | WP_002263087 |
| KGGNJBNG_7 | 1838 | 3121 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.84 | 99.61 | WP_000107753 |
| KGGNJBNG_23 | 20026 | 20609 | SPD_RS01775 | 1.32e-157 | Capsule | Immune modulation | 84.10 | 96.20 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KGGNJBNG_23 | 129850 | 128078 | spxB | 0.0 | pyruvate oxidase gene | 98.14 | 100.00 | AAB40976 |
| KGGNJBNG_27 | 34476 | 35459 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.34 | 100.00 | AAK75326 |
| KGGNJBNG_27 | 22892 | 21819 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| KGGNJBNG_12 | 142240 | 139934 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.28 | 99.22 | AAK74619 |
| KGGNJBNG_27 | 3350 | 1104 | metE | 0.0 | methionine biosynthesis enzymes | 93.19 | 100.00 | AAK74738 |
| KGGNJBNG_28 | 6155 | 7636 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.92 | 100.00 | ABJ54480 |
| KGGNJBNG_25 | 183241 | 184209 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| KGGNJBNG_4 | 3441 | 4412 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.36 | 100.00 | ABF36968 |
| KGGNJBNG_27 | 90966 | 84403 | PrtA | 0.0 | serine protease | 90.14 | 101.96 | ABJ55268 |
| KGGNJBNG_1 | 109444 | 107714 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| KGGNJBNG_26 | 119990 | 118839 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| KGGNJBNG_26 | 91547 | 89559 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.29 | 100.00 | AE014259_2 |
| KGGNJBNG_24 | 25673 | 27151 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| KGGNJBNG_1 | 250283 | 248982 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| KGGNJBNG_24 | 41021 | 42913 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.42 | 98.90 | ABP91129 |
| KGGNJBNG_23 | 225053 | 226762 | PGM | 0.0 | phosphoglucomutase | 84.76 | 99.65 | ACH87041 |
| KGGNJBNG_4 | 39912 | 41003 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| KGGNJBNG_24 | 186867 | 185857 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| KGGNJBNG_23 | 101886 | 103247 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.67 | 99.56 | ABP90420 |
| KGGNJBNG_1 | 264689 | 263799 | bglB | 1.86e-177 | putative phospho--glucosidase | 87.54 | 99.66 | AAK33487 |
| KGGNJBNG_23 | 185042 | 185992 | SP_0676 | 4.17e-170 | transcription facot | 81.39 | 98.45 | AAK74821 |
| KGGNJBNG_27 | 914 | 57 | metF | 6.45e-170 | methionine biosynthesis enzymes | 87.76 | 99.31 | AAK74739 |
| KGGNJBNG_5 | 1848 | 2552 | covR | 1.76e-127 | responder protein | 85.53 | 100.00 | AAK71319 |
| KGGNJBNG_27 | 109101 | 108430 | rr01 | 6.40e-127 | response regulator | 84.38 | 99.56 | CAB54566 |
| KGGNJBNG_23 | 220385 | 219714 | ciaR | 2.96e-124 | two component system response regulator | 85.71 | 99.12 | AIK75708 |
| KGGNJBNG_23 | 220385 | 219717 | CiaR | 8.37e-124 | two-component response regulator | 86.55 | 99.55 | AAK74935 |
| KGGNJBNG_5 | 1848 | 2552 | walR | 3.99e-121 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| KGGNJBNG_29 | 55318 | 56004 | CovR | 2.41e-120 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| KGGNJBNG_4 | 41176 | 41571 | SpxA2 | 4.10e-76 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| KGGNJBNG_23 | 90421 | 90020 | SpxA1 | 2.05e-69 | transcriptional regulator | 82.09 | 100.00 | ERL19273 |
| KGGNJBNG_3 | 6035 | 5622 | GrpE | 3.65e-68 | protein grpE | 81.88 | 80.59 | ABP89267 |
| KGGNJBNG_29 | 78524 | 78811 | spr0479 | 2.34e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| KGGNJBNG_25 | 150903 | 151157 | spr1327 | 8.91e-45 | essential gene | 92.94 | 100.00 | AAL00131 |
| KGGNJBNG_24 | 97624 | 97361 | spr0175 | 1.11e-42 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| KGGNJBNG_24 | 96798 | 96532 | spr0177 | 1.55e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| KGGNJBNG_12 | 142240 | 139940 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.09 | 98.08 | MYN69560 |
| KGGNJBNG_24 | 150175 | 151194 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.35 | 100.00 | ABJ55451 |
| KGGNJBNG_7 | 3288 | 3683 | RopE (M5005_Spy_1611) | 3.73e-70 | probable DNA-directed RNA polymerase subunit delta | 86.36 | 69.11 | AAK34603 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KGGNJBNG_1 | 0.0 | 1.0 | 0.0 |
| KGGNJBNG_2 | 0.0 | 1.0 | 0.0 |
| KGGNJBNG_3 | 0.0 | 1.0 | 0.0 |
| KGGNJBNG_4 | 0.0 | 1.0 | 0.0 |
| KGGNJBNG_5 | 0.0 | 1.0 | 0.0 |
| KGGNJBNG_6 | 0.0 | 1.0 | 0.0 |
| KGGNJBNG_7 | 0.0 | 1.0 | 0.0 |
| KGGNJBNG_9 | 1.0 | 0.0 | 1.0 |
| KGGNJBNG_10 | 0.0 | 1.0 | 0.0 |
| KGGNJBNG_11 | 1.0 | 0.0 | 1.0 |
| KGGNJBNG_12 | 0.0 | 1.0 | 0.0 |
| KGGNJBNG_13 | 0.0 | 1.0 | 0.0 |
| KGGNJBNG_23 | 0.0 | 1.0 | 0.0 |
| KGGNJBNG_24 | 0.0 | 1.0 | 0.0 |
| KGGNJBNG_25 | 0.0 | 1.0 | 0.0 |
| KGGNJBNG_26 | 0.0 | 1.0 | 0.0 |
| KGGNJBNG_27 | 0.0 | 1.0 | 0.0 |
| KGGNJBNG_28 | 0.0 | 1.0 | 0.0 |
| KGGNJBNG_29 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)