Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 25 | 33 | 41.40 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| NNGFEKGA_9 | 40394 | 41203 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.88 | 100.00 | WP_002948020 |
| NNGFEKGA_9 | 39454 | 40410 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.79 | 100.00 | WP_011226328 |
| NNGFEKGA_9 | 38309 | 39457 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | WP_041828290 |
| NNGFEKGA_9 | 33962 | 34813 | rfbD | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | WP_014621820 |
| NNGFEKGA_22 | 13174 | 14832 | fbp54 | 0.0 | FBPs | Adherence | 99.64 | 100.00 | WP_011227179 |
| NNGFEKGA_9 | 41203 | 42408 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | WP_011226326 |
| NNGFEKGA_16 | 10133 | 10972 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.52 | 100.00 | WP_011226733 |
| NNGFEKGA_9 | 37190 | 38200 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226330 |
| NNGFEKGA_9 | 42428 | 43483 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.43 | 100.00 | WP_002948022 |
| NNGFEKGA_3 | 19509 | 20378 | rfbA | 0.0 | Capsule | Immune modulation | 99.42 | 100.00 | WP_011681286 |
| NNGFEKGA_9 | 43480 | 45264 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.38 | 100.00 | WP_011226325 |
| NNGFEKGA_3 | 20378 | 20971 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | WP_011226149 |
| NNGFEKGA_3 | 20378 | 20971 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.16 | 100.00 | WP_011226149 |
| NNGFEKGA_3 | 20378 | 20971 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.16 | 100.00 | WP_011226149 |
| NNGFEKGA_17 | 6584 | 7279 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 98.99 | 100.29 | WP_011226047 |
| NNGFEKGA_17 | 6584 | 7279 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 98.42 | 100.29 | WP_011227247 |
| NNGFEKGA_4 | 992 | 1785 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.24 | 100.25 | - |
| NNGFEKGA_17 | 7288 | 8019 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.22 | 100.00 | WP_011226048 |
| NNGFEKGA_17 | 8020 | 9480 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 98.02 | 100.00 | WP_011681177 |
| NNGFEKGA_17 | 7288 | 8019 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 97.95 | 100.00 | WP_011681176 |
| NNGFEKGA_37 | 519 | 1934 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 97.88 | 100.00 | WP_011681165 |
| NNGFEKGA_10 | 30294 | 31055 | srtA | 0.0 | Sortase A | Exoenzyme | 97.64 | 100.00 | - |
| NNGFEKGA_17 | 7288 | 8019 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 97.54 | 100.00 | WP_011227248 |
| NNGFEKGA_9 | 35916 | 37187 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 97.48 | 99.76 | WP_011226331 |
| NNGFEKGA_17 | 8020 | 9480 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 97.26 | 100.21 | WP_011226049 |
| NNGFEKGA_17 | 8020 | 9480 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.30 | 100.21 | WP_041827043 |
| NNGFEKGA_17 | 5816 | 6574 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 95.52 | 100.94 | WP_011681174 |
| NNGFEKGA_17 | 5816 | 6574 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 95.39 | 100.94 | WP_011226046 |
| NNGFEKGA_17 | 4394 | 5759 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 95.24 | 99.85 | WP_011681173 |
| NNGFEKGA_17 | 5821 | 6574 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 94.56 | 99.07 | WP_011227246 |
| NNGFEKGA_17 | 4392 | 5759 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.01 | 100.00 | WP_011226045 |
| NNGFEKGA_17 | 6584 | 7279 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 88.94 | 100.29 | WP_011681175 |
| NNGFEKGA_1 | 76040 | 77343 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.17 | 99.54 | WP_000022832 |
| NNGFEKGA_26 | 1263 | 2273 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.44 | 99.41 | WP_002986042 |
| NNGFEKGA_57 | 82015 | 83298 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.73 | 99.77 | WP_000107753 |
| NNGFEKGA_3 | 21401 | 22447 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| NNGFEKGA_10 | 27607 | 26624 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| NNGFEKGA_11 | 13785 | 12712 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| NNGFEKGA_1 | 161028 | 160060 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| NNGFEKGA_5 | 15666 | 13363 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| NNGFEKGA_14 | 79614 | 78133 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.31 | 100.00 | ABJ54480 |
| NNGFEKGA_20 | 6177 | 8423 | metE | 0.0 | methionine biosynthesis enzymes | 91.72 | 100.00 | AAK74738 |
| NNGFEKGA_27 | 2015 | 1017 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| NNGFEKGA_16 | 27455 | 26484 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| NNGFEKGA_56 | 73084 | 74235 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| NNGFEKGA_9 | 18992 | 20980 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| NNGFEKGA_10 | 43815 | 45545 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| NNGFEKGA_25 | 28208 | 29686 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| NNGFEKGA_1 | 77343 | 76042 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| NNGFEKGA_25 | 43397 | 45289 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| NNGFEKGA_20 | 11348 | 9639 | PGM | 0.0 | phosphoglucomutase | 83.71 | 99.65 | ACH87041 |
| NNGFEKGA_19 | 2109 | 1099 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| NNGFEKGA_47 | 67571 | 68932 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| NNGFEKGA_6 | 5248 | 7140 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| NNGFEKGA_1 | 91778 | 90888 | bglB | 1.81e-179 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| NNGFEKGA_8 | 43804 | 44742 | SP_0676 | 5.99e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| NNGFEKGA_20 | 8615 | 9472 | metF | 1.40e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| NNGFEKGA_4 | 29465 | 30169 | covR | 1.29e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| NNGFEKGA_14 | 120806 | 121477 | rr01 | 2.08e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| NNGFEKGA_4 | 29465 | 30169 | walR | 5.45e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| NNGFEKGA_20 | 15302 | 15970 | CiaR | 1.87e-119 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| NNGFEKGA_2 | 9473 | 8787 | CovR | 4.49e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| NNGFEKGA_20 | 15302 | 15973 | ciaR | 1.84e-117 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| NNGFEKGA_27 | 741 | 367 | SpxA2 | 8.80e-72 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| NNGFEKGA_47 | 56211 | 55810 | SpxA1 | 2.50e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| NNGFEKGA_13 | 13854 | 14141 | spr0479 | 3.00e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| NNGFEKGA_1 | 195441 | 195187 | spr1327 | 3.75e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| NNGFEKGA_25 | 98651 | 98388 | spr0175 | 5.19e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| NNGFEKGA_25 | 97824 | 97558 | spr0177 | 9.17e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| NNGFEKGA_57 | 81857 | 81357 | RopE (M5005_Spy_1611) | 2.35e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| NNGFEKGA_5 | 15666 | 13369 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| NNGFEKGA_6 | 19720 | 20739 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| NNGFEKGA_1 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_2 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_3 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_4 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_5 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_6 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_7 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_8 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_9 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_10 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_11 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_12 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_13 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_14 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_15 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_16 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_17 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_18 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_19 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_20 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_21 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_22 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_23 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_24 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_25 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_26 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_27 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_28 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_29 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_30 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_31 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_32 | 1.0 | 0.0 | 1.0 |
| NNGFEKGA_34 | 1.0 | 0.0 | 1.0 |
| NNGFEKGA_35 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_36 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_37 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_38 | 1.0 | 0.0 | 1.0 |
| NNGFEKGA_39 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_40 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_41 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_42 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_43 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_44 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_45 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_46 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_47 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_48 | 1.0 | 0.0 | 1.0 |
| NNGFEKGA_56 | 0.0 | 1.0 | 0.0 |
| NNGFEKGA_57 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)