Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 25 | 33 | 41.40 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| GKEJJEIH_40 | 11157 | 12026 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| GKEJJEIH_40 | 10564 | 11157 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| GKEJJEIH_40 | 10564 | 11157 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| GKEJJEIH_23 | 16747 | 17703 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.79 | 100.00 | WP_011226328 |
| GKEJJEIH_23 | 15954 | 16763 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | WP_002948020 |
| GKEJJEIH_23 | 17700 | 18848 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | WP_041828290 |
| GKEJJEIH_40 | 10564 | 11157 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| GKEJJEIH_23 | 14749 | 15954 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | WP_011226326 |
| GKEJJEIH_23 | 11893 | 13677 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.55 | 100.00 | WP_011226325 |
| GKEJJEIH_23 | 22344 | 23195 | rfbD | 0.0 | Capsule | Immune modulation | 99.53 | 100.00 | WP_014621820 |
| GKEJJEIH_23 | 18957 | 19967 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226330 |
| GKEJJEIH_23 | 13674 | 14729 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.43 | 100.00 | WP_002948022 |
| GKEJJEIH_28 | 98143 | 99801 | fbp54 | 0.0 | FBPs | Adherence | 99.34 | 100.00 | WP_011227179 |
| GKEJJEIH_32 | 11439 | 12170 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | WP_011681176 |
| GKEJJEIH_32 | 11439 | 12170 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.77 | 100.00 | WP_011226048 |
| GKEJJEIH_32 | 11439 | 12170 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.63 | 100.00 | WP_011227248 |
| GKEJJEIH_32 | 78173 | 78961 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.36 | 99.75 | - |
| GKEJJEIH_32 | 1498 | 2913 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 97.81 | 100.00 | WP_011681165 |
| GKEJJEIH_33 | 14649 | 15410 | srtA | 0.0 | Sortase A | Exoenzyme | 97.64 | 100.00 | - |
| GKEJJEIH_38 | 58481 | 59440 | cbpD | 0.0 | Autolysin | Exoenzyme | 97.63 | 99.90 | WP_082302295 |
| GKEJJEIH_23 | 19970 | 21241 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 97.48 | 99.76 | WP_011226331 |
| GKEJJEIH_32 | 12171 | 13631 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.47 | 100.00 | WP_011681177 |
| GKEJJEIH_32 | 12171 | 13631 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.85 | 100.21 | WP_011226049 |
| GKEJJEIH_32 | 12171 | 13631 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 95.89 | 100.21 | WP_041827043 |
| GKEJJEIH_32 | 10738 | 11430 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 95.40 | 99.86 | WP_011227247 |
| GKEJJEIH_32 | 10738 | 11430 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 95.40 | 99.86 | WP_011226047 |
| GKEJJEIH_32 | 8548 | 9913 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 95.17 | 99.85 | WP_011681173 |
| GKEJJEIH_32 | 9970 | 10728 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 93.94 | 100.94 | WP_011681174 |
| GKEJJEIH_32 | 8546 | 9913 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 93.93 | 100.00 | WP_011226045 |
| GKEJJEIH_32 | 9970 | 10728 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 93.81 | 100.94 | WP_011226046 |
| GKEJJEIH_32 | 9975 | 10728 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 93.24 | 99.07 | WP_011227246 |
| GKEJJEIH_32 | 10738 | 11430 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.47 | 100.00 | WP_011681175 |
| GKEJJEIH_1 | 142878 | 144181 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| GKEJJEIH_11 | 14331 | 15341 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.45 | 99.90 | WP_002986042 |
| GKEJJEIH_12 | 59474 | 60757 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.65 | 99.77 | WP_000107753 |
| GKEJJEIH_40 | 9089 | 10135 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GKEJJEIH_33 | 18097 | 19080 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| GKEJJEIH_1 | 247268 | 248341 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| GKEJJEIH_1 | 59461 | 60429 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| GKEJJEIH_16 | 15603 | 13300 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| GKEJJEIH_35 | 27075 | 28556 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.31 | 100.00 | ABJ54480 |
| GKEJJEIH_39 | 21362 | 23608 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| GKEJJEIH_15 | 2088 | 1096 | recA | 0.0 | DNA recombination and repair | 91.24 | 85.31 | AAL00560 |
| GKEJJEIH_6 | 15876 | 14905 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| GKEJJEIH_16 | 129387 | 130538 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| GKEJJEIH_23 | 38486 | 36498 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| GKEJJEIH_33 | 1901 | 171 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| GKEJJEIH_4 | 27416 | 28894 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| GKEJJEIH_1 | 142878 | 144179 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| GKEJJEIH_4 | 42605 | 44497 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| GKEJJEIH_39 | 26533 | 24824 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| GKEJJEIH_5 | 7296 | 8306 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| GKEJJEIH_28 | 74572 | 75933 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| GKEJJEIH_4 | 131079 | 132971 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| GKEJJEIH_1 | 128443 | 129333 | bglB | 7.45e-178 | putative phospho--glucosidase | 88.22 | 99.66 | AAK33487 |
| GKEJJEIH_3 | 10532 | 11470 | SP_0676 | 2.77e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| GKEJJEIH_39 | 23800 | 24657 | metF | 1.50e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| GKEJJEIH_32 | 50339 | 49635 | covR | 2.90e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| GKEJJEIH_35 | 8099 | 7428 | rr01 | 1.78e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| GKEJJEIH_32 | 50339 | 49635 | walR | 1.22e-122 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| GKEJJEIH_39 | 30487 | 31155 | CiaR | 1.64e-119 | two-component response regulator | 84.75 | 99.55 | AAK74935 |
| GKEJJEIH_34 | 48078 | 47392 | CovR | 6.59e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| GKEJJEIH_39 | 30487 | 31158 | ciaR | 1.78e-117 | two component system response regulator | 82.59 | 99.12 | AIK75708 |
| GKEJJEIH_15 | 784 | 389 | SpxA2 | 8.17e-76 | transcriptional regulator | 90.15 | 100.00 | ERL21461 |
| GKEJJEIH_28 | 63213 | 62812 | SpxA1 | 3.00e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| GKEJJEIH_34 | 25699 | 25412 | spr0479 | 9.30e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| GKEJJEIH_1 | 25050 | 25304 | spr1327 | 3.89e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| GKEJJEIH_4 | 76247 | 75984 | spr0175 | 6.44e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| GKEJJEIH_4 | 75420 | 75154 | spr0177 | 1.41e-33 | essential gene | 84.27 | 88.12 | AAK98981 |
| GKEJJEIH_12 | 59316 | 58816 | RopE (M5005_Spy_1611) | 1.87e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| GKEJJEIH_16 | 15603 | 13306 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| GKEJJEIH_4 | 145482 | 146501 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 95.88 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GKEJJEIH_1 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_2 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_3 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_4 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_5 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_6 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_7 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_8 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_9 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_10 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_11 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_12 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_13 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_14 | 1.0 | 0.0 | 1.0 |
| GKEJJEIH_15 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_16 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_18 | 1.0 | 0.0 | 1.0 |
| GKEJJEIH_19 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_20 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_21 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_23 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_28 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_31 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_32 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_33 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_34 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_35 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_36 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_37 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_38 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_39 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_40 | 0.0 | 1.0 | 0.0 |
| GKEJJEIH_41 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)