Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 28 | 32 | 42.52 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DKDIEFKE_2 | 19509 | 20378 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| DKDIEFKE_2 | 20378 | 20971 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| DKDIEFKE_5 | 42937 | 43743 | STER_RS07060 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681437 |
| DKDIEFKE_5 | 34456 | 35715 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681445 |
| DKDIEFKE_5 | 40851 | 41999 | STER_RS07070 | 0.0 | Capsule | Immune modulation | 99.91 | 100.00 | WP_011681439 |
| DKDIEFKE_2 | 20378 | 20971 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| DKDIEFKE_5 | 41996 | 42937 | STR_RS06905 | 0.0 | Capsule | Immune modulation | 99.68 | 100.00 | WP_011227430 |
| DKDIEFKE_2 | 20378 | 20971 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| DKDIEFKE_5 | 46372 | 48117 | STER_RS07045 | 0.0 | Capsule | Immune modulation | 99.60 | 100.00 | WP_011681434 |
| DKDIEFKE_5 | 35712 | 36688 | STER_RS07095 | 0.0 | Capsule | Immune modulation | 99.59 | 99.90 | WP_011681444 |
| DKDIEFKE_36 | 86725 | 88383 | fbp54 | 0.0 | FBPs | Adherence | 99.58 | 100.00 | WP_011227179 |
| DKDIEFKE_5 | 41996 | 42937 | STER_RS07065 | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | WP_011681438 |
| DKDIEFKE_5 | 42937 | 43743 | STR_RS06900 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011227429 |
| DKDIEFKE_32 | 2364 | 3505 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 99.47 | 99.83 | - |
| DKDIEFKE_5 | 40851 | 41999 | STR_RS06910 | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | WP_011227431 |
| DKDIEFKE_5 | 36706 | 37674 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 99.28 | 100.00 | WP_011681443 |
| DKDIEFKE_17 | 14652 | 15413 | srtA | 0.0 | Sortase A | Exoenzyme | 99.08 | 100.00 | - |
| DKDIEFKE_25 | 46743 | 47711 | cbpD | 0.0 | Autolysin | Exoenzyme | 98.86 | 100.00 | WP_082302295 |
| DKDIEFKE_51 | 70035 | 70766 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.50 | 100.00 | WP_011681176 |
| DKDIEFKE_51 | 68580 | 70034 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 98.35 | 100.00 | WP_041827043 |
| DKDIEFKE_5 | 37676 | 38391 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 98.32 | 99.86 | WP_011681442 |
| DKDIEFKE_51 | 80779 | 82194 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 98.31 | 100.00 | WP_011681165 |
| DKDIEFKE_51 | 70035 | 70766 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.22 | 100.00 | WP_011226048 |
| DKDIEFKE_5 | 38737 | 39513 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 98.20 | 100.00 | WP_011681440 |
| DKDIEFKE_51 | 70035 | 70766 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.09 | 100.00 | WP_011227248 |
| DKDIEFKE_51 | 68580 | 70034 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 98.01 | 100.00 | WP_011226049 |
| DKDIEFKE_5 | 46372 | 48117 | STR_RS06885 | 0.0 | Capsule | Immune modulation | 97.94 | 100.00 | WP_011227426 |
| DKDIEFKE_51 | 71477 | 72217 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 97.84 | 100.00 | WP_011226046 |
| DKDIEFKE_51 | 71477 | 72217 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 97.71 | 100.00 | WP_011681174 |
| DKDIEFKE_51 | 70775 | 71467 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 97.69 | 100.00 | WP_011227247 |
| DKDIEFKE_51 | 70775 | 71467 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 97.69 | 100.00 | WP_011226047 |
| DKDIEFKE_5 | 33486 | 34337 | rfbD | 0.0 | Capsule | Immune modulation | 97.42 | 100.00 | WP_014621820 |
| DKDIEFKE_51 | 71477 | 72212 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 97.42 | 98.13 | WP_011227246 |
| DKDIEFKE_51 | 68580 | 70034 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.12 | 99.79 | WP_011681177 |
| DKDIEFKE_51 | 72274 | 73641 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 95.25 | 100.00 | WP_011226045 |
| DKDIEFKE_51 | 72274 | 73639 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 93.27 | 99.85 | WP_011681173 |
| DKDIEFKE_51 | 70775 | 71467 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 91.05 | 100.00 | WP_011681175 |
| DKDIEFKE_14 | 92461 | 93764 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| DKDIEFKE_27 | 123 | 1133 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.54 | 99.41 | WP_002986042 |
| DKDIEFKE_5 | 43743 | 44966 | SGO_RS05025 | 0.0 | Capsule | Immune modulation | 84.52 | 99.92 | WP_012000441 |
| DKDIEFKE_5 | 42937 | 43743 | SGO_RS05020 | 0.0 | Capsule | Immune modulation | 82.90 | 100.00 | WP_012000440 |
| DKDIEFKE_3 | 5276 | 6559 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.73 | 99.77 | WP_000107753 |
| DKDIEFKE_2 | 21400 | 22446 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| DKDIEFKE_51 | 83976 | 84562 | SPD_RS01775 | 5.33e-143 | Capsule | Immune modulation | 82.48 | 96.70 | WP_001819836 |
| DKDIEFKE_5 | 43743 | 44480 | STER_RS07055 | 0.0 | Capsule | Immune modulation | 98.10 | 61.35 | WP_011681436 |
| DKDIEFKE_5 | 43743 | 44480 | STR_RS06895 | 0.0 | Capsule | Immune modulation | 97.83 | 61.35 | WP_011227428 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DKDIEFKE_10 | 13785 | 12712 | MntE | 0.0 | manganese homeostasis | 94.97 | 99.72 | ABF32167 |
| DKDIEFKE_17 | 18100 | 19083 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| DKDIEFKE_7 | 40874 | 43177 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| DKDIEFKE_14 | 177453 | 176485 | spr0084 | 0.0 | pathogenitcity related | 92.88 | 98.48 | AAK98888 |
| DKDIEFKE_6 | 13101 | 11620 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.31 | 100.00 | ABJ54480 |
| DKDIEFKE_13 | 18913 | 21159 | metE | 0.0 | methionine biosynthesis enzymes | 91.86 | 100.00 | AAK74738 |
| DKDIEFKE_8 | 2058 | 1060 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| DKDIEFKE_8 | 39322 | 38351 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| DKDIEFKE_47 | 16907 | 15756 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| DKDIEFKE_5 | 20448 | 22436 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.69 | 100.00 | AE014259_2 |
| DKDIEFKE_17 | 1901 | 171 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| DKDIEFKE_1 | 173083 | 171605 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| DKDIEFKE_14 | 93764 | 92463 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| DKDIEFKE_1 | 157895 | 156003 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| DKDIEFKE_13 | 24084 | 22375 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| DKDIEFKE_1 | 21693 | 22703 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| DKDIEFKE_36 | 60257 | 61618 | mnmE | 0.0 | central tRNA-modifying GTPase | 81.58 | 99.56 | ABP90420 |
| DKDIEFKE_1 | 72028 | 70136 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| DKDIEFKE_14 | 108199 | 107309 | bglB | 1.37e-179 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| DKDIEFKE_18 | 10473 | 11411 | SP_0676 | 2.75e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| DKDIEFKE_13 | 21351 | 22208 | metF | 1.43e-167 | methionine biosynthesis enzymes | 86.71 | 99.31 | AAK74739 |
| DKDIEFKE_51 | 31733 | 32437 | covR | 2.10e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| DKDIEFKE_6 | 32077 | 32748 | rr01 | 9.36e-126 | response regulator | 83.93 | 99.56 | CAB54566 |
| DKDIEFKE_51 | 31733 | 32437 | walR | 8.86e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| DKDIEFKE_13 | 28038 | 28706 | CiaR | 3.26e-119 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| DKDIEFKE_52 | 73103 | 73789 | CovR | 4.37e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| DKDIEFKE_13 | 28038 | 28709 | ciaR | 3.21e-117 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| DKDIEFKE_8 | 784 | 410 | SpxA2 | 2.72e-71 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| DKDIEFKE_16 | 13963 | 14250 | spr0479 | 2.13e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| DKDIEFKE_25 | 5828 | 5574 | spr1327 | 1.35e-44 | essential gene | 94.12 | 100.00 | AAL00131 |
| DKDIEFKE_1 | 124251 | 124514 | spr0175 | 9.55e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| DKDIEFKE_1 | 125078 | 125344 | spr0177 | 1.69e-33 | essential gene | 84.27 | 88.12 | AAK98981 |
| DKDIEFKE_3 | 6717 | 7217 | RopE (M5005_Spy_1611) | 1.79e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| DKDIEFKE_7 | 40874 | 43171 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| DKDIEFKE_1 | 57577 | 56558 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DKDIEFKE_1 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_2 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_3 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_4 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_5 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_6 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_7 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_8 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_9 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_10 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_11 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_12 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_13 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_14 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_15 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_16 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_17 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_18 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_19 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_20 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_21 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_22 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_23 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_24 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_25 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_26 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_27 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_28 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_29 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_30 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_31 | 1.0 | 0.0 | 1.0 |
| DKDIEFKE_32 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_34 | 1.0 | 0.0 | 1.0 |
| DKDIEFKE_35 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_36 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_38 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_39 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_40 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_41 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_42 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_43 | 1.0 | 0.0 | 1.0 |
| DKDIEFKE_47 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_51 | 0.0 | 1.0 | 0.0 |
| DKDIEFKE_52 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DKDIEFKE_14 | 50833 | 92402 | False | Siphoviridae | VOG4649, VOG0054, VOG0641, VOG3298, VOG4782, VOG0647, VOG4599, VOG4605, VOG3636, VOG0800, VOG0725, VOG0799, VOG0724, VOG5660, VOG0723, VOG4713, VOG3435, VOG1887, VOG3434, VOG4564, VOG0720, VOG4544, VOG0796, VOG3299, VOG3300, VOG4052, VOG4694, VOG0198, VOG0658, VOG0657, VOG9667, VOG0152, VOG4571, VOG0656, VOG3468, VOG0655, VOG4693, VOG10904, VOG0226, VOG0685, VOG0283, VOG3430, VOG0189, VOG4566, VOG0651, VOG3478, VOG9469, VOG1564, VOG3622, VOG6127, VOG4602, VOG7656 |
Phigaro v2.4.0 (Database: Jan. 2024 release)