Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 27 | 35 | 44.20 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| OGJFNLFG_4 | 19509 | 20378 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| OGJFNLFG_4 | 20378 | 20971 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| OGJFNLFG_17 | 6454 | 7263 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.88 | 100.00 | WP_002948020 |
| OGJFNLFG_4 | 20378 | 20971 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| OGJFNLFG_17 | 7247 | 8203 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.79 | 100.00 | WP_011226328 |
| OGJFNLFG_17 | 8200 | 9348 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | WP_041828290 |
| OGJFNLFG_4 | 20378 | 20971 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| OGJFNLFG_17 | 12844 | 13695 | rfbD | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | WP_014621820 |
| OGJFNLFG_27 | 13186 | 14844 | fbp54 | 0.0 | FBPs | Adherence | 99.58 | 100.00 | WP_011227179 |
| OGJFNLFG_17 | 5249 | 6454 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | WP_011226326 |
| OGJFNLFG_1 | 1117 | 2260 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 99.56 | 100.00 | - |
| OGJFNLFG_18 | 21266 | 22105 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.52 | 100.00 | WP_011226733 |
| OGJFNLFG_60 | 13532 | 14131 | STR_RS05245 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_196767671 |
| OGJFNLFG_17 | 9457 | 10467 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226330 |
| OGJFNLFG_17 | 2393 | 4177 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.44 | 100.00 | WP_011226325 |
| OGJFNLFG_17 | 4174 | 5229 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.43 | 100.00 | WP_002948022 |
| OGJFNLFG_33 | 2537 | 3952 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | WP_011681165 |
| OGJFNLFG_60 | 14283 | 15018 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 98.91 | 99.33 | WP_011681174 |
| OGJFNLFG_12 | 30294 | 31055 | srtA | 0.0 | Sortase A | Exoenzyme | 98.82 | 100.00 | - |
| OGJFNLFG_60 | 15729 | 16460 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.77 | 100.00 | WP_011681176 |
| OGJFNLFG_60 | 16461 | 17915 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 98.76 | 100.00 | WP_041827043 |
| OGJFNLFG_60 | 12408 | 13367 | STR_RS05240 | 0.0 | Capsule | Immune modulation | 98.54 | 100.00 | WP_041827042 |
| OGJFNLFG_60 | 15729 | 16460 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.50 | 100.00 | WP_011226048 |
| OGJFNLFG_60 | 15729 | 16460 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.36 | 100.00 | WP_011227248 |
| OGJFNLFG_60 | 14269 | 15018 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 98.27 | 100.00 | WP_011227246 |
| OGJFNLFG_60 | 16461 | 17915 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 98.21 | 100.00 | WP_011226049 |
| OGJFNLFG_60 | 14283 | 15018 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 98.10 | 99.33 | WP_011226046 |
| OGJFNLFG_17 | 10470 | 11741 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 97.48 | 99.76 | WP_011226331 |
| OGJFNLFG_60 | 16461 | 17915 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 96.65 | 99.79 | WP_011681177 |
| OGJFNLFG_7 | 33312 | 38206 | SAG_RS09105 | 0.0 | Agglutinin receptor | Adherence | 92.87 | 99.98 | WP_000167699 |
| OGJFNLFG_7 | 33312 | 38254 | SSU98_0978 | 0.0 | Agglutinin receptor | Adherence | 92.82 | 99.78 | YP_001200536 |
| OGJFNLFG_7 | 33312 | 38254 | SSU05_0965 | 0.0 | Agglutinin receptor | Adherence | 92.80 | 99.78 | YP_001198331 |
| OGJFNLFG_60 | 15028 | 15720 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.90 | 100.00 | WP_011681175 |
| OGJFNLFG_60 | 15028 | 15720 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 89.75 | 100.00 | WP_011226047 |
| OGJFNLFG_60 | 15028 | 15720 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 89.47 | 100.00 | WP_011227247 |
| OGJFNLFG_1 | 104168 | 105471 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| OGJFNLFG_23 | 4317 | 5327 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.54 | 99.41 | WP_002986042 |
| OGJFNLFG_47 | 82016 | 83299 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.73 | 99.77 | WP_000107753 |
| OGJFNLFG_4 | 21400 | 22446 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| OGJFNLFG_12 | 27607 | 26624 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| OGJFNLFG_16 | 13785 | 12712 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| OGJFNLFG_7 | 38227 | 33317 | B9H01_RS04765 | 0.0 | glucan-binding protein | 93.40 | 99.63 | AUS90958 |
| OGJFNLFG_1 | 20483 | 21451 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| OGJFNLFG_8 | 15666 | 13363 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| OGJFNLFG_5 | 13173 | 11692 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.31 | 100.00 | ABJ54480 |
| OGJFNLFG_24 | 11392 | 9146 | metE | 0.0 | methionine biosynthesis enzymes | 91.72 | 100.00 | AAK74738 |
| OGJFNLFG_30 | 12642 | 13640 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| OGJFNLFG_18 | 4783 | 5754 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| OGJFNLFG_36 | 20026 | 18875 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| OGJFNLFG_17 | 28665 | 26677 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| OGJFNLFG_12 | 43806 | 45536 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| OGJFNLFG_25 | 27025 | 28503 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.22 | 100.00 | ADE32434 |
| OGJFNLFG_1 | 104168 | 105469 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| OGJFNLFG_25 | 42214 | 44106 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| OGJFNLFG_24 | 6221 | 7930 | PGM | 0.0 | phosphoglucomutase | 83.71 | 99.65 | ACH87041 |
| OGJFNLFG_21 | 2109 | 1099 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| OGJFNLFG_58 | 62187 | 63548 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| OGJFNLFG_9 | 48578 | 46686 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| OGJFNLFG_1 | 89733 | 90623 | bglB | 1.42e-179 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| OGJFNLFG_11 | 43973 | 44911 | SP_0676 | 6.01e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| OGJFNLFG_24 | 8954 | 8097 | metF | 1.40e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| OGJFNLFG_60 | 54758 | 54054 | covR | 1.98e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| OGJFNLFG_5 | 54365 | 55036 | rr01 | 1.20e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| OGJFNLFG_60 | 54758 | 54054 | walR | 8.34e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| OGJFNLFG_24 | 2267 | 1599 | CiaR | 1.87e-119 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| OGJFNLFG_2 | 8081 | 7395 | CovR | 4.41e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| OGJFNLFG_24 | 2267 | 1596 | ciaR | 1.84e-117 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| OGJFNLFG_30 | 13916 | 14290 | SpxA2 | 9.04e-72 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| OGJFNLFG_58 | 50828 | 50427 | SpxA1 | 1.75e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| OGJFNLFG_15 | 13761 | 14048 | spr0479 | 1.33e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| OGJFNLFG_3 | 56343 | 56597 | spr1327 | 1.11e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| OGJFNLFG_25 | 86766 | 86503 | spr0175 | 4.62e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| OGJFNLFG_25 | 85939 | 85673 | spr0177 | 8.17e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| OGJFNLFG_47 | 81858 | 81358 | RopE (M5005_Spy_1611) | 2.35e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| OGJFNLFG_8 | 15666 | 13369 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| OGJFNLFG_7 | 3604 | 2408 | AUS90997 (B9H01_RS07385) | 0.0 | site-specific integrase | 97.74 | 100.00 | AUS90997 |
| OGJFNLFG_9 | 34106 | 33087 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| OGJFNLFG_1 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_2 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_3 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_4 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_5 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_6 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_7 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_8 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_9 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_10 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_11 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_12 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_13 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_14 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_15 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_16 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_17 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_18 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_19 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_20 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_21 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_22 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_23 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_24 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_25 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_26 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_27 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_28 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_29 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_30 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_31 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_32 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_33 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_34 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_35 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_36 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_37 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_38 | 1.0 | 0.0 | 1.0 |
| OGJFNLFG_40 | 1.0 | 0.0 | 1.0 |
| OGJFNLFG_41 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_42 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_43 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_44 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_46 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_47 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_48 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_49 | 1.0 | 0.0 | 1.0 |
| OGJFNLFG_50 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_58 | 0.0 | 1.0 | 0.0 |
| OGJFNLFG_60 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| OGJFNLFG_1 | 105530 | 146579 | False | Siphoviridae | VOG0275, VOG7656, VOG4602, VOG6127, VOG3622, VOG1564, VOG9469, VOG3478, VOG0651, VOG4566, VOG0189, VOG3430, VOG0283, VOG0685, VOG0226, VOG10904, VOG4693, VOG0655, VOG3468, VOG0656, VOG0152, VOG0656, VOG4571, VOG0152, VOG9667, VOG0657, VOG0658, VOG0198, VOG0796, VOG4544, VOG5047, VOG0720, VOG4564, VOG4555, VOG3434, VOG1887, VOG3435, VOG4713, VOG0723, VOG5660, VOG0724, VOG0799, VOG0725, VOG0800, VOG5793, VOG4605, VOG4599, VOG0647, VOG4782, VOG3298, VOG3424, VOG0054, VOG4649 |
Phigaro v2.4.0 (Database: Jan. 2024 release)