Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 8 | 17.55 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DMFPBFCN_2 | 428588 | 428905 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| DMFPBFCN_2 | 428250 | 428588 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| DMFPBFCN_3 | 412117 | 413760 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.00 | NC_000964.3 |
| DMFPBFCN_4 | 299321 | 300175 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| DMFPBFCN_4 | 280183 | 281385 | blt | ARO:3003006 | blt | 98.75 | 100.00 | L32599.1 |
| DMFPBFCN_5 | 268573 | 271143 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| DMFPBFCN_3 | 161704 | 162297 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| DMFPBFCN_3 | 111146 | 112579 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| DMFPBFCN_3 | 98331 | 99251 | mphK | ARO:3004541 | mphK | 98.69 | 100.00 | CP010314.1 |
| DMFPBFCN_4 | 56401 | 57570 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DMFPBFCN_1 | 1011661 | 1013037 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.42 | 100.00 | D12567 |
| DMFPBFCN_3 | 98331 | 99251 | mph(K) | Spiramycin, Telithromycin | 99.13 | 100.00 | NC_000964 |
| DMFPBFCN_4 | 299323 | 300175 | aadK | Streptomycin | 98.12 | 99.77 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DMFPBFCN_1 | 1011664 | 1013037 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.34 | 100.00 | WP_063856081.1 |
| DMFPBFCN_3 | 98331 | 99248 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.69 | 100.00 | WP_003246254.1 |
| DMFPBFCN_3 | 412120 | 413760 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| DMFPBFCN_4 | 299324 | 300175 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| DMFPBFCN_1 | 1009133 | 1009651 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DMFPBFCN_1 | 527367 | 528548 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| DMFPBFCN_1 | 102014 | 109150 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.62 | 100.00 | NP_391076 |
| DMFPBFCN_7 | 153936 | 154577 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| DMFPBFCN_1 | 113006 | 113791 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| DMFPBFCN_1 | 109170 | 110108 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391077 |
| DMFPBFCN_1 | 111784 | 112980 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.17 | 100.00 | NP_391079 |
| DMFPBFCN_7 | 8369 | 10132 | dep/capD | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | NP_389723 |
| DMFPBFCN_1 | 110136 | 111755 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.07 | 100.00 | NP_391078 |
| DMFPBFCN_1 | 525742 | 526884 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| DMFPBFCN_1 | 526903 | 527352 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DMFPBFCN_12 | 31733 | 32869 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| DMFPBFCN_15 | 4359 | 5228 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| DMFPBFCN_2 | 738401 | 739177 | CodY | 7.49e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DMFPBFCN_1 | 366006 | 366575 | ClpP | 6.30e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| DMFPBFCN_2 | 276368 | 276760 | spxA1 | 5.89e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| DMFPBFCN_2 | 276368 | 276760 | spx | 3.51e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| DMFPBFCN_11 | 20595 | 20837 | SpoVG | 7.52e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| DMFPBFCN_3 | 362560 | 362757 | CspA | 1.91e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| DMFPBFCN_7 | 166540 | 166734 | CspD | 1.33e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DMFPBFCN_1 | 0.0 | 1.0 | 0.0 |
| DMFPBFCN_2 | 0.0 | 1.0 | 0.0 |
| DMFPBFCN_3 | 0.0 | 1.0 | 0.0 |
| DMFPBFCN_4 | 0.0 | 1.0 | 0.0 |
| DMFPBFCN_5 | 0.0 | 1.0 | 0.0 |
| DMFPBFCN_6 | 0.0 | 1.0 | 0.0 |
| DMFPBFCN_7 | 0.0 | 1.0 | 0.0 |
| DMFPBFCN_8 | 0.0 | 1.0 | 0.0 |
| DMFPBFCN_9 | 0.0 | 1.0 | 0.0 |
| DMFPBFCN_10 | 0.0 | 1.0 | 0.0 |
| DMFPBFCN_11 | 0.0 | 1.0 | 0.0 |
| DMFPBFCN_12 | 0.0 | 1.0 | 0.0 |
| DMFPBFCN_13 | 0.0 | 1.0 | 0.0 |
| DMFPBFCN_14 | 0.0 | 1.0 | 0.0 |
| DMFPBFCN_15 | 0.0 | 1.0 | 0.0 |
| DMFPBFCN_16 | 0.0 | 1.0 | 0.0 |
| DMFPBFCN_17 | 0.0 | 1.0 | 0.0 |
| DMFPBFCN_19 | 1.0 | 0.0 | 1.0 |
| DMFPBFCN_20 | 0.0 | 1.0 | 0.0 |
| DMFPBFCN_21 | 1.0 | 0.0 | 1.0 |
| DMFPBFCN_22 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DMFPBFCN_2 | 371194 | 398766 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705 |
| DMFPBFCN_4 | 229613 | 261612 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4662, VOG4673, VOG0143, VOG4693, VOG0189, VOG11060, VOG1309 |
Phigaro v2.4.0 (Database: Jan. 2024 release)