Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 7 | 17.83 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DEBPEKCJ_11 | 582868 | 583722 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| DEBPEKCJ_11 | 564103 | 565305 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| DEBPEKCJ_14 | 550823 | 551140 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| DEBPEKCJ_14 | 550485 | 550823 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| DEBPEKCJ_11 | 372464 | 373633 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| DEBPEKCJ_10 | 258162 | 260732 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| DEBPEKCJ_9 | 197613 | 199256 | vmlR | ARO:3004476 | vmlR | 98.90 | 100.00 | NC_000964.3 |
| DEBPEKCJ_8 | 150917 | 151510 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| DEBPEKCJ_8 | 100358 | 101797 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.42 | JYFL01000006.1 |
| DEBPEKCJ_8 | 87543 | 88463 | mphK | ARO:3004541 | mphK | 98.37 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DEBPEKCJ_13 | 1027495 | 1028871 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.78 | 100.00 | D12567 |
| DEBPEKCJ_11 | 582870 | 583722 | aadK | Streptomycin | 98.71 | 99.77 | M26879 |
| DEBPEKCJ_8 | 87543 | 88463 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DEBPEKCJ_13 | 1027498 | 1028871 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.78 | 100.00 | WP_063856081.1 |
| DEBPEKCJ_9 | 197616 | 199256 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.90 | 100.00 | WP_003234144.1 |
| DEBPEKCJ_8 | 87543 | 88460 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.37 | 100.00 | WP_003246254.1 |
| DEBPEKCJ_11 | 582871 | 583722 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| DEBPEKCJ_13 | 1025132 | 1025551 | satA | streptothricin N-acetyltransferase SatA | PARTIALX | 89.29 | 80.92 | WP_003242546.1 |
| DEBPEKCJ_11 | 59163 | 61760 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.94 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DEBPEKCJ_11 | 9245 | 11008 | dep/capD | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_389723 |
| DEBPEKCJ_13 | 524310 | 525491 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| DEBPEKCJ_13 | 112949 | 113734 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.62 | 100.00 | NP_391080 |
| DEBPEKCJ_13 | 109113 | 110051 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.57 | 100.00 | NP_391077 |
| DEBPEKCJ_13 | 110079 | 111698 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.51 | 100.00 | NP_391078 |
| DEBPEKCJ_13 | 522685 | 523827 | capA | 0.0 | Capsule | Immune modulation | 99.47 | 100.00 | NP_391469 |
| DEBPEKCJ_11 | 169442 | 170083 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| DEBPEKCJ_13 | 101957 | 109093 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.34 | 100.00 | NP_391076 |
| DEBPEKCJ_13 | 523846 | 524295 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| DEBPEKCJ_13 | 111727 | 112923 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DEBPEKCJ_6 | 28094 | 26958 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| DEBPEKCJ_21 | 11309 | 10440 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| DEBPEKCJ_14 | 861006 | 861782 | CodY | 9.43e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DEBPEKCJ_13 | 365192 | 365761 | ClpP | 6.41e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| DEBPEKCJ_14 | 276450 | 276842 | spxA1 | 7.41e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| DEBPEKCJ_14 | 276450 | 276842 | spx | 4.42e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| DEBPEKCJ_5 | 34760 | 34518 | SpoVG | 7.52e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| DEBPEKCJ_9 | 145953 | 146150 | CspA | 9.86e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DEBPEKCJ_2 | 0.0 | 1.0 | 0.0 |
| DEBPEKCJ_3 | 0.0 | 1.0 | 0.0 |
| DEBPEKCJ_4 | 0.0 | 1.0 | 0.0 |
| DEBPEKCJ_5 | 0.0 | 1.0 | 0.0 |
| DEBPEKCJ_6 | 0.0 | 1.0 | 0.0 |
| DEBPEKCJ_8 | 0.0 | 1.0 | 0.0 |
| DEBPEKCJ_9 | 0.0 | 1.0 | 0.0 |
| DEBPEKCJ_10 | 0.0 | 1.0 | 0.0 |
| DEBPEKCJ_11 | 0.0 | 1.0 | 0.0 |
| DEBPEKCJ_13 | 0.0 | 1.0 | 0.0 |
| DEBPEKCJ_14 | 0.0 | 1.0 | 0.0 |
| DEBPEKCJ_20 | 0.0 | 1.0 | 0.0 |
| DEBPEKCJ_21 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DEBPEKCJ_14 | 314078 | 324214 | False | Unknown | VOG0026, VOG8598, VOG8506, VOG4551, VOG0025, VOG0189 |
| DEBPEKCJ_14 | 402336 | 406314 | False | Siphoviridae | VOG9226, VOG5986, VOG0052, VOG0535 |
| DEBPEKCJ_14 | 494177 | 520965 | False | Myoviridae | VOG4602, VOG9667, VOG10599, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG4612, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)