Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 9 | 17.38 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BOJLDBFB_11 | 604515 | 605369 | aadK | ARO:3002627 | aadK | 98.94 | 100.00 | AL009126.1 |
| BOJLDBFB_11 | 575027 | 576229 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| BOJLDBFB_20 | 423371 | 423688 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| BOJLDBFB_20 | 423033 | 423371 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| BOJLDBFB_11 | 385122 | 386291 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| BOJLDBFB_25 | 109318 | 110238 | mphK | ARO:3004541 | mphK | 98.37 | 100.00 | CP010314.1 |
| BOJLDBFB_25 | 95988 | 97427 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.42 | JYFL01000006.1 |
| BOJLDBFB_25 | 46370 | 46963 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| BOJLDBFB_24 | 28788 | 30431 | vmlR | ARO:3004476 | vmlR | 98.72 | 100.00 | NC_000964.3 |
| BOJLDBFB_23 | 27694 | 30264 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.42 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BOJLDBFB_11 | 604515 | 605369 | aadK | Streptomycin | 98.71 | 100.00 | M26879 |
| BOJLDBFB_25 | 109318 | 110238 | mph(K) | Spiramycin, Telithromycin | 97.39 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BOJLDBFB_11 | 604518 | 605369 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.94 | 100.00 | WP_003229862.1 |
| BOJLDBFB_24 | 28788 | 30428 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.72 | 100.00 | WP_003234144.1 |
| BOJLDBFB_25 | 109321 | 110238 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.37 | 100.00 | WP_003246254.1 |
| BOJLDBFB_26 | 96003 | 98600 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.48 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BOJLDBFB_19 | 97249 | 98034 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| BOJLDBFB_19 | 506493 | 507674 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| BOJLDBFB_11 | 130562 | 131203 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| BOJLDBFB_19 | 504868 | 506010 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| BOJLDBFB_19 | 506029 | 506478 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| BOJLDBFB_19 | 96027 | 97223 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.91 | 100.00 | NP_391079 |
| BOJLDBFB_19 | 86257 | 93393 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.61 | 100.00 | NP_391076 |
| BOJLDBFB_26 | 150006 | 151769 | dep/capD | 0.0 | Capsule | Immune modulation | 98.53 | 100.00 | NP_389723 |
| BOJLDBFB_19 | 93413 | 94351 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.40 | 100.00 | NP_391077 |
| BOJLDBFB_19 | 94379 | 95998 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.27 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BOJLDBFB_2 | 28031 | 26895 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| BOJLDBFB_6 | 11247 | 10378 | pdxS | 1.55e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| BOJLDBFB_21 | 155957 | 156733 | CodY | 3.00e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BOJLDBFB_19 | 349997 | 350566 | ClpP | 4.31e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| BOJLDBFB_20 | 275644 | 276036 | spxA1 | 3.69e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| BOJLDBFB_20 | 275644 | 276036 | spx | 2.20e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| BOJLDBFB_3 | 20589 | 20831 | SpoVG | 7.50e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BOJLDBFB_24 | 86062 | 85865 | CspA | 9.71e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| BOJLDBFB_11 | 143167 | 143361 | CspD | 6.42e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| BOJLDBFB_20 | 33149 | 32955 | CspB | 5.32e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BOJLDBFB_1 | 0.0 | 1.0 | 0.0 |
| BOJLDBFB_2 | 0.0 | 1.0 | 0.0 |
| BOJLDBFB_3 | 0.0 | 1.0 | 0.0 |
| BOJLDBFB_4 | 0.0 | 1.0 | 0.0 |
| BOJLDBFB_5 | 0.0 | 1.0 | 0.0 |
| BOJLDBFB_6 | 0.0 | 1.0 | 0.0 |
| BOJLDBFB_7 | 0.0 | 1.0 | 0.0 |
| BOJLDBFB_8 | 0.0 | 1.0 | 0.0 |
| BOJLDBFB_9 | 0.0 | 1.0 | 0.0 |
| BOJLDBFB_11 | 0.0 | 1.0 | 0.0 |
| BOJLDBFB_12 | 1.0 | 0.0 | 1.0 |
| BOJLDBFB_14 | 0.0 | 1.0 | 0.0 |
| BOJLDBFB_19 | 0.0 | 1.0 | 0.0 |
| BOJLDBFB_20 | 0.0 | 1.0 | 0.0 |
| BOJLDBFB_21 | 0.0 | 1.0 | 0.0 |
| BOJLDBFB_22 | 0.0 | 1.0 | 0.0 |
| BOJLDBFB_23 | 0.0 | 1.0 | 0.0 |
| BOJLDBFB_24 | 0.0 | 1.0 | 0.0 |
| BOJLDBFB_25 | 0.0 | 1.0 | 0.0 |
| BOJLDBFB_26 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BOJLDBFB_4 | 21054 | 47515 | False | Myoviridae | VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG1581, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG5668, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG0906, VOG7718, VOG4705 |
| BOJLDBFB_11 | 86987 | 106032 | False | Siphoviridae | VOG3390, VOG7895, VOG8610, VOG0275, VOG9312, VOG4910, VOG3389, VOG5042, VOG9712, VOG7900 |
| BOJLDBFB_11 | 285751 | 315564 | False | Siphoviridae | VOG1030, VOG8261, VOG1093, VOG5543, VOG4877, VOG8598, VOG0052, VOG4551, VOG0025, VOG0189, VOG6168, VOG10230, VOG0641, VOG0565, VOG4599, VOG4605, VOG4545, VOG5716, VOG7238, VOG5293, VOG10063, VOG4319, VOG6031, VOG0692, VOG0720 |
| BOJLDBFB_20 | 366036 | 393489 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)