Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 8 | 10 | 4 | 13.42 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HDGLLJGE_1 | 2208298 | 2209467 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| HDGLLJGE_1 | 1316388 | 1316705 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| HDGLLJGE_1 | 1316050 | 1316388 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| HDGLLJGE_1 | 837399 | 839969 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| HDGLLJGE_1 | 561675 | 563318 | vmlR | ARO:3004476 | vmlR | 97.99 | 100.00 | NC_000964.3 |
| HDGLLJGE_1 | 274159 | 275592 | lmrB | ARO:3002813 | lmrB | 98.74 | 100.00 | JYFL01000006.1 |
| HDGLLJGE_1 | 264173 | 265087 | mphK | ARO:3004541 | mphK | 97.39 | 99.35 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| HDGLLJGE_1 | 264173 | 265087 | mph(K) | Spiramycin, Telithromycin | 97.18 | 99.35 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HDGLLJGE_1 | 561678 | 563318 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 97.99 | 100.00 | WP_003234144.1 |
| HDGLLJGE_1 | 264173 | 265084 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.39 | 100.00 | WP_003246254.1 |
| HDGLLJGE_1 | 3807305 | 3807823 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HDGLLJGE_1 | 3344024 | 3345205 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| HDGLLJGE_1 | 2937864 | 2938802 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.47 | 100.00 | NP_391077 |
| HDGLLJGE_1 | 2941689 | 2942474 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| HDGLLJGE_1 | 1859739 | 1861502 | dep/capD | 0.0 | Capsule | Immune modulation | 99.26 | 100.00 | NP_389723 |
| HDGLLJGE_1 | 3342399 | 3343541 | capA | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_391469 |
| HDGLLJGE_1 | 3343560 | 3344009 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| HDGLLJGE_1 | 2930708 | 2937844 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.99 | 100.00 | NP_391076 |
| HDGLLJGE_1 | 2940467 | 2941663 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.83 | 100.00 | NP_391079 |
| HDGLLJGE_1 | 2005181 | 2005822 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 97.82 | 100.00 | NP_390062 |
| HDGLLJGE_1 | 2938830 | 2940449 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.78 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HDGLLJGE_1 | 132727 | 133863 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| HDGLLJGE_1 | 18662 | 19531 | pdxS | 2.85e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| HDGLLJGE_1 | 1635188 | 1635964 | CodY | 3.11e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| HDGLLJGE_1 | 3199893 | 3200462 | ClpP | 2.31e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HDGLLJGE_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| HDGLLJGE_1 | 1260134 | 1286818 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705, VOG0753 |
Phigaro v2.4.0 (Database: Jan. 2024 release)