Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 4 | 16.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GHJPKFHL_1 | 2482500 | 2483354 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| GHJPKFHL_1 | 2464062 | 2465264 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| GHJPKFHL_1 | 2282922 | 2284091 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| GHJPKFHL_1 | 1397217 | 1397534 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| GHJPKFHL_1 | 1396879 | 1397217 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| GHJPKFHL_1 | 904560 | 907130 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| GHJPKFHL_1 | 601330 | 602976 | vmlR | ARO:3004476 | vmlR | 98.36 | 100.18 | NC_000964.3 |
| GHJPKFHL_1 | 335678 | 336271 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| GHJPKFHL_1 | 287847 | 289280 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.00 | JYFL01000006.1 |
| GHJPKFHL_1 | 275033 | 275953 | mphK | ARO:3004541 | mphK | 98.69 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| GHJPKFHL_1 | 4026197 | 4027573 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.13 | 100.00 | D12567 |
| GHJPKFHL_1 | 2482500 | 2483354 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| GHJPKFHL_1 | 275033 | 275953 | mph(K) | Spiramycin, Telithromycin | 98.37 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GHJPKFHL_1 | 4026200 | 4027573 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.13 | 100.00 | WP_063856081.1 |
| GHJPKFHL_1 | 275033 | 275950 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.69 | 100.00 | WP_003246254.1 |
| GHJPKFHL_1 | 2482503 | 2483354 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| GHJPKFHL_1 | 601333 | 602976 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.36 | 100.00 | WP_003234144.1 |
| GHJPKFHL_1 | 4023677 | 4024195 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| GHJPKFHL_1 | 3522189 | 3523370 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| GHJPKFHL_1 | 3028749 | 3029534 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.62 | 100.00 | NP_391080 |
| GHJPKFHL_1 | 3520564 | 3521706 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| GHJPKFHL_1 | 3024913 | 3025851 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391077 |
| GHJPKFHL_1 | 1947473 | 1949236 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| GHJPKFHL_1 | 2064619 | 2065260 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| GHJPKFHL_1 | 3521725 | 3522174 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| GHJPKFHL_1 | 3017757 | 3024893 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.61 | 100.00 | NP_391076 |
| GHJPKFHL_1 | 3027527 | 3028723 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.83 | 100.00 | NP_391079 |
| GHJPKFHL_1 | 3025879 | 3027498 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.28 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GHJPKFHL_1 | 132531 | 133667 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| GHJPKFHL_1 | 18600 | 19469 | pdxS | 3.01e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| GHJPKFHL_1 | 1714088 | 1714864 | CodY | 3.29e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| GHJPKFHL_1 | 3372181 | 3372750 | ClpP | 2.44e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GHJPKFHL_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| GHJPKFHL_1 | 1340741 | 1367423 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| GHJPKFHL_1 | 3208678 | 3248615 | True | Siphoviridae | VOG0641, VOG4705, VOG6243, VOG4612, VOG4626, VOG4772, VOG0801, VOG5910, VOG0541, VOG4586, VOG0704, VOG9583, VOG0204, VOG10223, VOG4553, VOG4568, VOG4556, VOG3607, VOG1886, VOG4581, VOG0713, VOG4841, VOG0275, VOG0198, VOG0944, VOG5532, VOG0044, VOG0102, VOG0397, VOG5836, VOG0245, VOG1336, VOG6587, VOG0189, VOG4566, VOG7581, VOG9667, VOG9667, VOG1563, VOG0275, VOG0275 |
| GHJPKFHL_1 | 3337346 | 3358608 | False | Siphoviridae | VOG0602, VOG4574, VOG5221, VOG4705, VOG6243, VOG4612, VOG4626, VOG4619, VOG0801, VOG11074, VOG4319, VOG9317, VOG0692, VOG2613, VOG3699, VOG5023, VOG0275 |
Phigaro v2.4.0 (Database: Jan. 2024 release)