Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 24 | 31 | 39.26 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DKBOHLFD_1 | 1354199 | 1354936 | ErmB | ARO:3000375 | ErmB | 97.96 | 98.79 | AF242872.1 |
| DKBOHLFD_1 | 1351355 | 1353274 | tet(M) | ARO:3000186 | tet(M) | 99.06 | 100.00 | AB039845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DKBOHLFD_1 | 1354199 | 1354936 | erm(B) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 100.00 | U86375 |
| DKBOHLFD_1 | 1351355 | 1353274 | tet(M) | Doxycycline, Tetracycline, Minocycline | 99.53 | 100.00 | X75073 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DKBOHLFD_1 | 1354199 | 1354933 | erm(B) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) | EXACTX | 100.00 | 100.00 | WP_001038790.1 |
| DKBOHLFD_1 | 1351355 | 1353271 | tet(M) | tetracycline resistance ribosomal protection protein Tet(M) | BLASTX | 99.84 | 100.00 | WP_000691736.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DKBOHLFD_1 | 952654 | 953394 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 95.28 | 100.00 | WP_011681174 |
| DKBOHLFD_1 | 1618144 | 1618995 | rfbD | 0.0 | Capsule | Immune modulation | 95.07 | 100.00 | WP_014621820 |
| DKBOHLFD_1 | 952654 | 953394 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 94.60 | 100.00 | WP_011226046 |
| DKBOHLFD_1 | 953452 | 954819 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.30 | 100.00 | WP_011226045 |
| DKBOHLFD_1 | 952654 | 953389 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 94.02 | 98.13 | WP_011227246 |
| DKBOHLFD_1 | 953452 | 954817 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.00 | 99.85 | WP_011681173 |
| DKBOHLFD_1 | 1615772 | 1617043 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 93.95 | 99.76 | WP_011226331 |
| DKBOHLFD_1 | 949751 | 951211 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 93.91 | 100.00 | WP_011681177 |
| DKBOHLFD_1 | 949751 | 951211 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 93.70 | 100.14 | WP_011226049 |
| DKBOHLFD_1 | 949751 | 951211 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 93.09 | 100.14 | WP_041827043 |
| DKBOHLFD_1 | 1610203 | 1611012 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 92.96 | 100.00 | WP_002948020 |
| DKBOHLFD_1 | 1606163 | 1607947 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 92.94 | 100.00 | WP_011226325 |
| DKBOHLFD_1 | 1614811 | 1615769 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 92.81 | 94.86 | WP_011226330 |
| DKBOHLFD_1 | 1610996 | 1611952 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 92.48 | 100.00 | WP_011226328 |
| DKBOHLFD_1 | 1608998 | 1610203 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 92.45 | 100.00 | WP_011226326 |
| DKBOHLFD_1 | 951212 | 951943 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 91.94 | 100.00 | WP_011226048 |
| DKBOHLFD_1 | 1611949 | 1613097 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 91.66 | 99.83 | WP_041828290 |
| DKBOHLFD_1 | 951212 | 951943 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 91.53 | 100.00 | WP_011681176 |
| DKBOHLFD_1 | 774887 | 775756 | rfbA | 0.0 | Capsule | Immune modulation | 91.38 | 100.00 | WP_011681286 |
| DKBOHLFD_1 | 951212 | 951943 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 90.98 | 100.00 | WP_011227248 |
| DKBOHLFD_1 | 36821 | 37660 | cbpD | 0.0 | Autolysin | Exoenzyme | 90.95 | 100.00 | WP_011226733 |
| DKBOHLFD_1 | 775756 | 776349 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| DKBOHLFD_1 | 1607944 | 1608978 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 90.91 | 99.15 | WP_002948022 |
| DKBOHLFD_1 | 775756 | 776349 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 90.74 | 100.00 | WP_011226149 |
| DKBOHLFD_1 | 775756 | 776349 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 90.74 | 100.00 | WP_011226149 |
| DKBOHLFD_1 | 1072623 | 1074281 | fbp54 | 0.0 | FBPs | Adherence | 90.42 | 99.94 | WP_011227179 |
| DKBOHLFD_1 | 951952 | 952644 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 88.49 | 99.71 | WP_011227247 |
| DKBOHLFD_1 | 951952 | 952644 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 88.49 | 99.71 | WP_011226047 |
| DKBOHLFD_1 | 604785 | 606088 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.17 | 99.54 | WP_000022832 |
| DKBOHLFD_1 | 951952 | 952644 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 87.48 | 99.71 | WP_011681175 |
| DKBOHLFD_1 | 1932979 | 1933989 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.36 | 99.80 | WP_002986042 |
| DKBOHLFD_1 | 1223514 | 1224275 | srtA | 0.0 | Sortase A | Exoenzyme | 84.16 | 99.74 | - |
| DKBOHLFD_1 | 776614 | 777659 | rfbB | 0.0 | Capsule | Immune modulation | 81.12 | 99.81 | WP_009659423 |
| DKBOHLFD_1 | 133904 | 135187 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.61 | 99.61 | WP_000107753 |
| DKBOHLFD_1 | 964932 | 965515 | SPD_RS01775 | 1.29e-146 | Capsule | Immune modulation | 83.08 | 96.20 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DKBOHLFD_1 | 1226963 | 1227937 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 95.69 | 99.09 | AAK75326 |
| DKBOHLFD_1 | 1215379 | 1214306 | MntE | 0.0 | manganese homeostasis | 95.25 | 99.72 | ABF32167 |
| DKBOHLFD_1 | 1809764 | 1812070 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.54 | 99.22 | AAK74619 |
| DKBOHLFD_1 | 1394352 | 1395833 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.32 | 100.00 | ABJ54480 |
| DKBOHLFD_1 | 515495 | 516463 | spr0084 | 0.0 | pathogenitcity related | 92.88 | 98.48 | AAK98888 |
| DKBOHLFD_1 | 1186839 | 1184593 | metE | 0.0 | methionine biosynthesis enzymes | 92.12 | 100.00 | AAK74738 |
| DKBOHLFD_1 | 22399 | 23370 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.36 | 100.00 | ABF36968 |
| DKBOHLFD_1 | 1279918 | 1273355 | PrtA | 0.0 | serine protease | 90.05 | 101.96 | ABJ55268 |
| DKBOHLFD_1 | 749511 | 751241 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| DKBOHLFD_1 | 1661060 | 1659909 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| DKBOHLFD_1 | 1632405 | 1630417 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.44 | 100.00 | AE014259_2 |
| DKBOHLFD_1 | 2165004 | 2163526 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| DKBOHLFD_1 | 604785 | 606086 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| DKBOHLFD_1 | 2149545 | 2147653 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.58 | 98.90 | ABP91129 |
| DKBOHLFD_1 | 1181675 | 1183384 | PGM | 0.0 | phosphoglucomutase | 84.76 | 99.65 | ACH87041 |
| DKBOHLFD_1 | 58687 | 59778 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| DKBOHLFD_1 | 1987829 | 1988839 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| DKBOHLFD_1 | 1046810 | 1048171 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.67 | 99.56 | ABP90420 |
| DKBOHLFD_1 | 590379 | 591269 | bglB | 2.71e-177 | putative phospho--glucosidase | 87.88 | 99.66 | AAK33487 |
| DKBOHLFD_1 | 1140792 | 1141730 | SP_0676 | 1.87e-168 | transcription facot | 81.79 | 97.20 | AAK74821 |
| DKBOHLFD_1 | 1184407 | 1183550 | metF | 5.87e-167 | methionine biosynthesis enzymes | 86.71 | 99.31 | AAK74739 |
| DKBOHLFD_1 | 1090030 | 1089245 | pnuC | 9.15e-149 | NAD salvage pathway importer | 87.02 | 97.40 | AAK75931 |
| DKBOHLFD_1 | 904661 | 905365 | covR | 3.45e-125 | responder protein | 85.11 | 100.00 | AAK71319 |
| DKBOHLFD_1 | 1304555 | 1303884 | rr01 | 1.57e-124 | response regulator | 83.93 | 99.56 | CAB54566 |
| DKBOHLFD_1 | 1177707 | 1177036 | ciaR | 2.09e-124 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| DKBOHLFD_1 | 1177707 | 1177039 | CiaR | 3.86e-124 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| DKBOHLFD_1 | 278331 | 279017 | CovR | 4.77e-119 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| DKBOHLFD_1 | 904661 | 905365 | walR | 6.34e-119 | DNA-binding response regulator | 81.70 | 100.00 | ANC99504 |
| DKBOHLFD_1 | 59951 | 60346 | SpxA2 | 2.15e-74 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| DKBOHLFD_1 | 1035321 | 1034920 | SpxA1 | 2.41e-68 | transcriptional regulator | 82.09 | 100.00 | ERL19273 |
| DKBOHLFD_1 | 120637 | 121050 | GrpE | 4.01e-66 | protein grpE | 81.16 | 80.59 | ABP89267 |
| DKBOHLFD_1 | 135354 | 135845 | RopE (M5005_Spy_1611) | 1.05e-71 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| DKBOHLFD_1 | 1809764 | 1812064 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.22 | 98.08 | MYN69560 |
| DKBOHLFD_1 | 2024233 | 2023214 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.35 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| DKBOHLFD_1 | 1341873 | 1343078 | repUS43 | 1206 / 1206 | 100.00 | CP003584 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DKBOHLFD_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)