Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 20 | 29 | 35.28 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| NHFCAKPK_1 | 1520125 | 1521342 | mef(A) | Erythromycin, Azithromycin | 100.00 | 100.00 | U83667 |
| NHFCAKPK_1 | 1518477 | 1519940 | msr(D) | Erythromycin, Azithromycin, Telithromycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.18 | 100.00 | AF274302 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| NHFCAKPK_1 | 1520128 | 1521342 | mef(A) | macrolide efflux MFS transporter Mef(A) | EXACTX | 100.00 | 100.00 | WP_000417519.1 |
| NHFCAKPK_1 | 1518480 | 1519940 | msr(D) | ABC-F type ribosomal protection protein Msr(D) | BLASTX | 98.77 | 100.00 | WP_000420313.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| NHFCAKPK_1 | 529686 | 530401 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 96.65 | 99.86 | WP_011681442 |
| NHFCAKPK_1 | 1179129 | 1180494 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 95.46 | 99.85 | WP_011681173 |
| NHFCAKPK_1 | 1179127 | 1180494 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.96 | 100.00 | WP_011226045 |
| NHFCAKPK_1 | 525525 | 526376 | rfbD | 0.0 | Capsule | Immune modulation | 94.95 | 100.00 | WP_014621820 |
| NHFCAKPK_1 | 528716 | 529684 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 94.94 | 100.00 | WP_011681443 |
| NHFCAKPK_1 | 1182734 | 1184194 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 94.25 | 100.14 | WP_011226049 |
| NHFCAKPK_1 | 1180551 | 1181291 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 94.20 | 100.00 | WP_011681174 |
| NHFCAKPK_1 | 1182734 | 1184194 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 94.11 | 100.00 | WP_011681177 |
| NHFCAKPK_1 | 1182734 | 1184194 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 93.36 | 100.14 | WP_041827043 |
| NHFCAKPK_1 | 1356466 | 1357059 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 93.27 | 100.00 | WP_011226149 |
| NHFCAKPK_1 | 1180551 | 1181291 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 93.12 | 100.00 | WP_011226046 |
| NHFCAKPK_1 | 1356466 | 1357059 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 93.10 | 100.00 | WP_011226149 |
| NHFCAKPK_1 | 1356466 | 1357059 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 93.10 | 100.00 | WP_011226149 |
| NHFCAKPK_1 | 1180556 | 1181291 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 92.94 | 98.13 | WP_011227246 |
| NHFCAKPK_1 | 530747 | 531523 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 92.66 | 100.00 | WP_011681440 |
| NHFCAKPK_1 | 526495 | 527748 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 92.35 | 99.52 | WP_011681445 |
| NHFCAKPK_1 | 1182002 | 1182733 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 91.53 | 100.00 | WP_011226048 |
| NHFCAKPK_1 | 1357059 | 1357928 | rfbA | 0.0 | Capsule | Immune modulation | 91.49 | 100.00 | WP_011681286 |
| NHFCAKPK_1 | 1182002 | 1182733 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 91.26 | 100.00 | WP_011681176 |
| NHFCAKPK_1 | 2141623 | 2142462 | cbpD | 0.0 | Autolysin | Exoenzyme | 91.07 | 100.00 | WP_011226733 |
| NHFCAKPK_1 | 1069135 | 1070793 | fbp54 | 0.0 | FBPs | Adherence | 90.84 | 99.94 | WP_011227179 |
| NHFCAKPK_1 | 1182002 | 1182733 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 90.57 | 100.00 | WP_011227248 |
| NHFCAKPK_1 | 210888 | 211898 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 88.75 | 99.80 | WP_002986042 |
| NHFCAKPK_1 | 1540884 | 1542187 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.47 | 99.54 | WP_000022832 |
| NHFCAKPK_1 | 1181301 | 1181993 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 87.90 | 99.86 | WP_011227247 |
| NHFCAKPK_1 | 1181301 | 1181993 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 87.90 | 99.86 | WP_011226047 |
| NHFCAKPK_1 | 1181301 | 1181993 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 87.61 | 99.86 | WP_011681175 |
| NHFCAKPK_1 | 918439 | 919200 | srtA | 0.0 | Sortase A | Exoenzyme | 84.16 | 99.74 | - |
| NHFCAKPK_1 | 2044905 | 2046188 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.68 | 99.61 | WP_000107753 |
| NHFCAKPK_1 | 1355217 | 1356263 | rfbB | 0.0 | Capsule | Immune modulation | 80.36 | 99.90 | WP_009659423 |
| NHFCAKPK_1 | 1169450 | 1170033 | SPD_RS01775 | 5.90e-155 | Capsule | Immune modulation | 83.93 | 96.20 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| NHFCAKPK_1 | 915751 | 914777 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 95.69 | 99.09 | AAK75326 |
| NHFCAKPK_1 | 928397 | 929470 | MntE | 0.0 | manganese homeostasis | 95.53 | 99.72 | ABF32167 |
| NHFCAKPK_1 | 337964 | 335658 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.54 | 99.22 | AAK74619 |
| NHFCAKPK_1 | 763840 | 762359 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.12 | 100.00 | ABJ54480 |
| NHFCAKPK_1 | 1633258 | 1632290 | spr0084 | 0.0 | pathogenitcity related | 92.88 | 98.48 | AAK98888 |
| NHFCAKPK_1 | 956450 | 958696 | metE | 0.0 | methionine biosynthesis enzymes | 92.52 | 100.00 | AAK74738 |
| NHFCAKPK_1 | 2156852 | 2155881 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| NHFCAKPK_1 | 1383305 | 1381575 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 90.12 | 100.00 | AAK34199 |
| NHFCAKPK_1 | 511630 | 513618 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.59 | 100.00 | AE014259_2 |
| NHFCAKPK_1 | 483144 | 484295 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| NHFCAKPK_1 | 13565 | 15043 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| NHFCAKPK_1 | 1542187 | 1540886 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| NHFCAKPK_1 | 28919 | 30811 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.26 | 98.90 | ABP91129 |
| NHFCAKPK_1 | 961616 | 959907 | PGM | 0.0 | phosphoglucomutase | 84.94 | 99.65 | ACH87041 |
| NHFCAKPK_1 | 2122323 | 2121232 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| NHFCAKPK_1 | 155492 | 154482 | pdh | 0.0 | pyruvate dehydrogenase | 82.79 | 99.70 | ABP91006 |
| NHFCAKPK_1 | 1084315 | 1082954 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.67 | 99.56 | ABP90420 |
| NHFCAKPK_1 | 1557847 | 1556957 | bglB | 2.26e-177 | putative phospho--glucosidase | 87.88 | 99.66 | AAK33487 |
| NHFCAKPK_1 | 958883 | 959740 | metF | 9.84e-169 | methionine biosynthesis enzymes | 88.11 | 99.31 | AAK74739 |
| NHFCAKPK_1 | 1002477 | 1001527 | SP_0676 | 5.41e-168 | transcription facot | 81.07 | 98.45 | AAK74821 |
| NHFCAKPK_1 | 843731 | 844402 | rr01 | 3.03e-126 | response regulator | 84.82 | 99.56 | CAB54566 |
| NHFCAKPK_1 | 1229682 | 1228978 | covR | 3.45e-125 | responder protein | 85.11 | 100.00 | AAK71319 |
| NHFCAKPK_1 | 965583 | 966254 | ciaR | 7.00e-124 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| NHFCAKPK_1 | 965583 | 966251 | CiaR | 9.78e-124 | two-component response regulator | 87.00 | 99.55 | AAK74935 |
| NHFCAKPK_1 | 1890274 | 1889588 | CovR | 4.77e-119 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| NHFCAKPK_1 | 1229682 | 1228978 | walR | 6.34e-119 | DNA-binding response regulator | 81.70 | 100.00 | ANC99504 |
| NHFCAKPK_1 | 2121058 | 2120663 | SpxA2 | 2.15e-74 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| NHFCAKPK_1 | 1095784 | 1096185 | SpxA1 | 4.47e-69 | transcriptional regulator | 82.84 | 100.00 | ERL19273 |
| NHFCAKPK_1 | 2061673 | 2061260 | GrpE | 4.01e-66 | protein grpE | 81.16 | 80.59 | ABP89267 |
| NHFCAKPK_1 | 2044738 | 2044247 | RopE (M5005_Spy_1611) | 2.86e-71 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| NHFCAKPK_1 | 337964 | 335664 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.22 | 98.08 | MYN69560 |
| NHFCAKPK_1 | 118790 | 119809 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.06 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| NHFCAKPK_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)