Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 24 | 31 | 39.26 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MKPKCPNB_1 | 1354362 | 1355099 | ErmB | ARO:3000375 | ErmB | 97.96 | 98.79 | AF242872.1 |
| MKPKCPNB_1 | 1351518 | 1353437 | tet(M) | ARO:3000186 | tet(M) | 99.06 | 100.00 | AB039845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MKPKCPNB_1 | 1354362 | 1355099 | erm(B) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 100.00 | U86375 |
| MKPKCPNB_1 | 1351518 | 1353437 | tet(M) | Doxycycline, Tetracycline, Minocycline | 99.53 | 100.00 | X75073 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MKPKCPNB_1 | 1354362 | 1355096 | erm(B) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) | EXACTX | 100.00 | 100.00 | WP_001038790.1 |
| MKPKCPNB_1 | 1351518 | 1353434 | tet(M) | tetracycline resistance ribosomal protection protein Tet(M) | BLASTX | 99.84 | 100.00 | WP_000691736.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MKPKCPNB_1 | 952817 | 953557 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 95.28 | 100.00 | WP_011681174 |
| MKPKCPNB_1 | 1618305 | 1619156 | rfbD | 0.0 | Capsule | Immune modulation | 95.07 | 100.00 | WP_014621820 |
| MKPKCPNB_1 | 952817 | 953557 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 94.60 | 100.00 | WP_011226046 |
| MKPKCPNB_1 | 953615 | 954982 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.30 | 100.00 | WP_011226045 |
| MKPKCPNB_1 | 952817 | 953552 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 94.02 | 98.13 | WP_011227246 |
| MKPKCPNB_1 | 953615 | 954980 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.00 | 99.85 | WP_011681173 |
| MKPKCPNB_1 | 1615933 | 1617204 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 93.95 | 99.76 | WP_011226331 |
| MKPKCPNB_1 | 949914 | 951374 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 93.91 | 100.00 | WP_011681177 |
| MKPKCPNB_1 | 949914 | 951374 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 93.70 | 100.14 | WP_011226049 |
| MKPKCPNB_1 | 949914 | 951374 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 93.09 | 100.14 | WP_041827043 |
| MKPKCPNB_1 | 1610364 | 1611173 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 92.96 | 100.00 | WP_002948020 |
| MKPKCPNB_1 | 1606324 | 1608108 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 92.94 | 100.00 | WP_011226325 |
| MKPKCPNB_1 | 1614972 | 1615930 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 92.81 | 94.86 | WP_011226330 |
| MKPKCPNB_1 | 1611157 | 1612113 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 92.48 | 100.00 | WP_011226328 |
| MKPKCPNB_1 | 1609159 | 1610364 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 92.45 | 100.00 | WP_011226326 |
| MKPKCPNB_1 | 951375 | 952106 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 91.94 | 100.00 | WP_011226048 |
| MKPKCPNB_1 | 1612110 | 1613258 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 91.66 | 99.83 | WP_041828290 |
| MKPKCPNB_1 | 951375 | 952106 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 91.53 | 100.00 | WP_011681176 |
| MKPKCPNB_1 | 775049 | 775918 | rfbA | 0.0 | Capsule | Immune modulation | 91.38 | 100.00 | WP_011681286 |
| MKPKCPNB_1 | 951375 | 952106 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 90.98 | 100.00 | WP_011227248 |
| MKPKCPNB_1 | 37025 | 37864 | cbpD | 0.0 | Autolysin | Exoenzyme | 90.95 | 100.00 | WP_011226733 |
| MKPKCPNB_1 | 775918 | 776511 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| MKPKCPNB_1 | 1608105 | 1609139 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 90.91 | 99.15 | WP_002948022 |
| MKPKCPNB_1 | 775918 | 776511 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 90.74 | 100.00 | WP_011226149 |
| MKPKCPNB_1 | 775918 | 776511 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 90.74 | 100.00 | WP_011226149 |
| MKPKCPNB_1 | 1072786 | 1074444 | fbp54 | 0.0 | FBPs | Adherence | 90.42 | 99.94 | WP_011227179 |
| MKPKCPNB_1 | 952115 | 952807 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 88.49 | 99.71 | WP_011227247 |
| MKPKCPNB_1 | 952115 | 952807 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 88.49 | 99.71 | WP_011226047 |
| MKPKCPNB_1 | 604999 | 606302 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.17 | 99.54 | WP_000022832 |
| MKPKCPNB_1 | 952115 | 952807 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 87.48 | 99.71 | WP_011681175 |
| MKPKCPNB_1 | 1933117 | 1934127 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.36 | 99.80 | WP_002986042 |
| MKPKCPNB_1 | 1223676 | 1224437 | srtA | 0.0 | Sortase A | Exoenzyme | 84.16 | 99.74 | - |
| MKPKCPNB_1 | 776776 | 777822 | rfbB | 0.0 | Capsule | Immune modulation | 81.12 | 99.90 | WP_009659423 |
| MKPKCPNB_1 | 134108 | 135391 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.61 | 99.61 | WP_000107753 |
| MKPKCPNB_1 | 965095 | 965678 | SPD_RS01775 | 1.29e-146 | Capsule | Immune modulation | 83.08 | 96.20 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MKPKCPNB_1 | 1227125 | 1228099 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 95.69 | 99.09 | AAK75326 |
| MKPKCPNB_1 | 1215541 | 1214468 | MntE | 0.0 | manganese homeostasis | 95.25 | 99.72 | ABF32167 |
| MKPKCPNB_1 | 1809902 | 1812208 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.54 | 99.22 | AAK74619 |
| MKPKCPNB_1 | 1394514 | 1395995 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.32 | 100.00 | ABJ54480 |
| MKPKCPNB_1 | 515709 | 516677 | spr0084 | 0.0 | pathogenitcity related | 92.88 | 98.48 | AAK98888 |
| MKPKCPNB_1 | 1187002 | 1184756 | metE | 0.0 | methionine biosynthesis enzymes | 92.12 | 100.00 | AAK74738 |
| MKPKCPNB_1 | 22603 | 23574 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.36 | 100.00 | ABF36968 |
| MKPKCPNB_1 | 1280080 | 1273517 | PrtA | 0.0 | serine protease | 90.05 | 101.96 | ABJ55268 |
| MKPKCPNB_1 | 749673 | 751403 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| MKPKCPNB_1 | 1661222 | 1660071 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| MKPKCPNB_1 | 1632566 | 1630578 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.44 | 100.00 | AE014259_2 |
| MKPKCPNB_1 | 2165028 | 2163550 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| MKPKCPNB_1 | 604999 | 606300 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| MKPKCPNB_1 | 2149681 | 2147789 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.58 | 98.90 | ABP91129 |
| MKPKCPNB_1 | 1181838 | 1183547 | PGM | 0.0 | phosphoglucomutase | 84.76 | 99.65 | ACH87041 |
| MKPKCPNB_1 | 58891 | 59982 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| MKPKCPNB_1 | 1987966 | 1988976 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| MKPKCPNB_1 | 1046973 | 1048334 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.67 | 99.56 | ABP90420 |
| MKPKCPNB_1 | 590593 | 591483 | bglB | 2.71e-177 | putative phospho--glucosidase | 87.88 | 99.66 | AAK33487 |
| MKPKCPNB_1 | 1140955 | 1141893 | SP_0676 | 1.87e-168 | transcription facot | 81.79 | 97.20 | AAK74821 |
| MKPKCPNB_1 | 1184570 | 1183713 | metF | 5.87e-167 | methionine biosynthesis enzymes | 86.71 | 99.31 | AAK74739 |
| MKPKCPNB_1 | 1090193 | 1089408 | pnuC | 9.15e-149 | NAD salvage pathway importer | 87.02 | 97.40 | AAK75931 |
| MKPKCPNB_1 | 904824 | 905528 | covR | 3.45e-125 | responder protein | 85.11 | 100.00 | AAK71319 |
| MKPKCPNB_1 | 1304717 | 1304046 | rr01 | 1.57e-124 | response regulator | 83.93 | 99.56 | CAB54566 |
| MKPKCPNB_1 | 1177870 | 1177199 | ciaR | 2.09e-124 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| MKPKCPNB_1 | 1177870 | 1177202 | CiaR | 3.86e-124 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| MKPKCPNB_1 | 278548 | 279234 | CovR | 4.77e-119 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| MKPKCPNB_1 | 904824 | 905528 | walR | 6.33e-119 | DNA-binding response regulator | 81.70 | 100.00 | ANC99504 |
| MKPKCPNB_1 | 60155 | 60550 | SpxA2 | 2.15e-74 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| MKPKCPNB_1 | 1035484 | 1035083 | SpxA1 | 2.41e-68 | transcriptional regulator | 82.09 | 100.00 | ERL19273 |
| MKPKCPNB_1 | 120841 | 121254 | GrpE | 4.01e-66 | protein grpE | 81.16 | 80.59 | ABP89267 |
| MKPKCPNB_1 | 135558 | 136049 | RopE (M5005_Spy_1611) | 1.05e-71 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| MKPKCPNB_1 | 1809902 | 1812202 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.22 | 98.08 | MYN69560 |
| MKPKCPNB_1 | 2024370 | 2023351 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.35 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| MKPKCPNB_1 | 1342036 | 1343241 | repUS43 | 1206 / 1206 | 100.00 | CP003584 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MKPKCPNB_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)