Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 9 | 16.76 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IELDDGJM_56 | 145383 | 146822 | lmrB | ARO:3002813 | lmrB | 98.74 | 100.42 | JYFL01000006.1 |
| IELDDGJM_67 | 100058 | 100375 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| IELDDGJM_111 | 99820 | 100989 | bmr | ARO:3003007 | bmr | 98.97 | 100.00 | M33768.1 |
| IELDDGJM_67 | 99720 | 100058 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| IELDDGJM_40 | 4845 | 7415 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| IELDDGJM_91 | 219 | 1073 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| IELDDGJM_28 | 2798 | 4174 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.35 | 100.00 | X08034 |
| IELDDGJM_91 | 221 | 1073 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
| IELDDGJM_56 | 119096 | 120015 | mph(K) | Spiramycin, Telithromycin | 97.61 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IELDDGJM_28 | 2798 | 4171 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.13 | 100.00 | WP_003242953.1 |
| IELDDGJM_91 | 222 | 1073 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| IELDDGJM_36 | 44605 | 46245 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 97.62 | 100.00 | WP_003234144.1 |
| IELDDGJM_28 | 6155 | 6673 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 92.49 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| IELDDGJM_57 | 3209 | 4390 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| IELDDGJM_57 | 1584 | 2726 | capA | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | NP_391469 |
| IELDDGJM_57 | 2745 | 3194 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| IELDDGJM_89 | 21319 | 22104 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| IELDDGJM_61 | 1425 | 3188 | dep/capD | 0.0 | Capsule | Immune modulation | 99.26 | 100.00 | NP_389723 |
| IELDDGJM_89 | 25960 | 33096 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.95 | 100.00 | NP_391076 |
| IELDDGJM_89 | 22130 | 23326 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.75 | 100.00 | NP_391079 |
| IELDDGJM_89 | 25002 | 25940 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.62 | 100.00 | NP_391077 |
| IELDDGJM_89 | 23355 | 24974 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.90 | 100.00 | NP_391078 |
| IELDDGJM_2 | 97703 | 98344 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 97.82 | 100.00 | NP_390062 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IELDDGJM_35 | 31654 | 32790 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| IELDDGJM_79 | 11121 | 10252 | pdxS | 1.92e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| IELDDGJM_34 | 115611 | 114835 | CodY | 2.01e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| IELDDGJM_1 | 161037 | 161606 | ClpP | 1.87e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| IELDDGJM_37 | 38631 | 38239 | spxA1 | 3.72e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| IELDDGJM_37 | 38631 | 38239 | spx | 2.22e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| IELDDGJM_41 | 20512 | 20754 | SpoVG | 7.49e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| IELDDGJM_85 | 10783 | 10980 | CspA | 4.81e-33 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| IELDDGJM_2 | 85742 | 85548 | CspD | 6.46e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| IELDDGJM_45 | 13045 | 12851 | CspB | 1.87e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| IELDDGJM_94 | 1 | 191 | Col(SD853) | 191 / 194 | 97.91 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IELDDGJM_1 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_2 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_13 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_24 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_27 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_28 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_29 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_30 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_31 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_32 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_33 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_34 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_35 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_36 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_37 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_38 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_39 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_40 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_41 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_42 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_43 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_44 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_45 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_46 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_47 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_48 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_49 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_50 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_51 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_52 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_53 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_54 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_55 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_56 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_57 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_58 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_59 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_60 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_61 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_62 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_63 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_64 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_65 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_66 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_67 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_68 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_69 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_70 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_71 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_72 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_73 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_74 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_75 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_76 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_77 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_78 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_79 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_80 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_81 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_82 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_83 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_84 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_85 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_86 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_87 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_88 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_89 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_90 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_91 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_92 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_93 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_94 | 1.0 | 0.0 | 1.0 |
| IELDDGJM_95 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_96 | 1.0 | 0.0 | 1.0 |
| IELDDGJM_97 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_98 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_99 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_100 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_101 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_102 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_103 | 1.0 | 0.0 | 1.0 |
| IELDDGJM_104 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_105 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_107 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_108 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_109 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_110 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_111 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_112 | 1.0 | 0.0 | 1.0 |
| IELDDGJM_114 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_115 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_116 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_117 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_118 | 1.0 | 0.0 | 1.0 |
| IELDDGJM_119 | 0.0 | 1.0 | 0.0 |
| IELDDGJM_120 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| IELDDGJM_24 | 12840 | 35948 | False | Myoviridae | VOG0796, VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| IELDDGJM_42 | 24845 | 45527 | False | Siphoviridae | VOG4574, VOG0136, VOG0753, VOG10940, VOG5535, VOG4599, VOG4605, VOG9745, VOG0701, VOG10044, VOG6548, VOG0697, VOG0696, VOG0695, VOG2621, VOG4572, VOG9317, VOG0692, VOG0691, VOG10227, VOG0796, VOG7983, VOG0198 |
| IELDDGJM_67 | 44627 | 69723 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| IELDDGJM_100 | 18098 | 27923 | False | Siphoviridae | VOG1198, VOG0918, VOG4551, VOG0025, VOG7476 |
| IELDDGJM_100 | 91199 | 111725 | False | Unknown | VOG8610, VOG7930, VOG0275, VOG9312, VOG4910, VOG3389, VOG5042, VOG9712, VOG5070, VOG10230 |
Phigaro v2.4.0 (Database: Jan. 2024 release)