Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 7 | 17.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OPFBFCNJ_12 | 537852 | 538706 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| OPFBFCNJ_12 | 520754 | 521956 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| OPFBFCNJ_23 | 431004 | 431321 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| OPFBFCNJ_23 | 430666 | 431004 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| OPFBFCNJ_12 | 347522 | 348691 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| OPFBFCNJ_27 | 270752 | 273322 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| OPFBFCNJ_28 | 191677 | 193323 | vmlR | ARO:3004476 | vmlR | 99.27 | 100.18 | NC_000964.3 |
| OPFBFCNJ_29 | 107967 | 108887 | mphK | ARO:3004541 | mphK | 99.02 | 100.00 | CP010314.1 |
| OPFBFCNJ_29 | 94634 | 96073 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.42 | JYFL01000006.1 |
| OPFBFCNJ_29 | 44962 | 45555 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| OPFBFCNJ_29 | 107967 | 108887 | mph(K) | Spiramycin, Telithromycin | 99.35 | 100.00 | NC_000964 |
| OPFBFCNJ_12 | 537852 | 538706 | aadK | Streptomycin | 98.71 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OPFBFCNJ_28 | 191680 | 193323 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.27 | 100.00 | WP_003234144.1 |
| OPFBFCNJ_29 | 107970 | 108887 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.02 | 100.00 | WP_003246254.1 |
| OPFBFCNJ_12 | 537855 | 538706 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| OPFBFCNJ_1 | 66587 | 67105 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.06 | 100.00 | WP_003242546.1 |
| OPFBFCNJ_12 | 54004 | 56601 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.41 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| OPFBFCNJ_1 | 559050 | 560231 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| OPFBFCNJ_1 | 961049 | 961834 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| OPFBFCNJ_12 | 150356 | 150997 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| OPFBFCNJ_1 | 964732 | 965670 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391077 |
| OPFBFCNJ_12 | 8258 | 10021 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| OPFBFCNJ_1 | 560714 | 561856 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| OPFBFCNJ_1 | 965690 | 972826 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.92 | 100.00 | NP_391076 |
| OPFBFCNJ_1 | 560246 | 560695 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| OPFBFCNJ_1 | 961860 | 963056 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.66 | 100.00 | NP_391079 |
| OPFBFCNJ_1 | 963085 | 964704 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.91 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| OPFBFCNJ_31 | 31640 | 32776 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| OPFBFCNJ_2 | 11170 | 10301 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| OPFBFCNJ_23 | 742512 | 743288 | CodY | 7.58e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| OPFBFCNJ_1 | 701709 | 701140 | ClpP | 6.46e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| OPFBFCNJ_23 | 275339 | 275731 | spxA1 | 5.96e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| OPFBFCNJ_23 | 275339 | 275731 | spx | 3.56e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| OPFBFCNJ_32 | 20481 | 20723 | SpoVG | 7.48e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| OPFBFCNJ_28 | 145352 | 145549 | CspA | 9.60e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| OPFBFCNJ_1 | 0.0 | 1.0 | 0.0 |
| OPFBFCNJ_2 | 0.0 | 1.0 | 0.0 |
| OPFBFCNJ_3 | 0.0 | 1.0 | 0.0 |
| OPFBFCNJ_4 | 0.0 | 1.0 | 0.0 |
| OPFBFCNJ_5 | 0.0 | 1.0 | 0.0 |
| OPFBFCNJ_8 | 0.0 | 1.0 | 0.0 |
| OPFBFCNJ_12 | 0.0 | 1.0 | 0.0 |
| OPFBFCNJ_23 | 0.0 | 1.0 | 0.0 |
| OPFBFCNJ_27 | 0.0 | 1.0 | 0.0 |
| OPFBFCNJ_28 | 0.0 | 1.0 | 0.0 |
| OPFBFCNJ_29 | 0.0 | 1.0 | 0.0 |
| OPFBFCNJ_30 | 0.0 | 1.0 | 0.0 |
| OPFBFCNJ_31 | 0.0 | 1.0 | 0.0 |
| OPFBFCNJ_32 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| OPFBFCNJ_23 | 374836 | 401152 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)