Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 1 | 2 | 8 | 8.31 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MLFELBAE_11 | 23697 | 24746 | clbA | ARO:3002814 | clbA | 99.43 | 100.00 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MLFELBAE_11 | 23711 | 24746 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.90 | 98.67 | KR610408 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MLFELBAE_11 | 23700 | 24746 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 99.43 | 100.00 | WP_012116915.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MLFELBAE_3 | 28579 | 29760 | capB | 0.0 | Capsule | Immune modulation | 81.96 | 99.66 | NP_391471 |
| MLFELBAE_3 | 28115 | 28564 | capC | 1.13e-114 | Capsule | Immune modulation | 83.59 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MLFELBAE_16 | 31768 | 32904 | tufA | 0.0 | elongation factor Tu | 86.81 | 95.69 | AHM69299 |
| MLFELBAE_22 | 12498 | 11629 | pdxS | 8.82e-153 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| MLFELBAE_1 | 54722 | 55498 | CodY | 9.54e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MLFELBAE_4 | 169951 | 169559 | spxA1 | 6.56e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| MLFELBAE_11 | 47845 | 47648 | CspA | 1.04e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| MLFELBAE_18 | 38627 | 38433 | CspB | 1.08e-27 | cold shock protein | 84.61 | 98.48 | CAD00094 |
| MLFELBAE_1 | 569439 | 569633 | CspD | 2.07e-27 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| MLFELBAE_11 | 47845 | 47651 | CspR | 1.36e-26 | cold shock protein | 80.00 | 98.48 | AAO82613 |
| MLFELBAE_9 | 91646 | 92518 | lipA (SAUSA300_0829) | 7.41e-163 | lipoic acid synthetase | 80.82 | 95.41 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MLFELBAE_1 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_2 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_3 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_4 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_5 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_6 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_7 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_8 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_9 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_10 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_11 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_12 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_13 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_14 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_15 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_16 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_17 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_18 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_19 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_20 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_21 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_22 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_27 | 0.0 | 1.0 | 0.0 |
| MLFELBAE_28 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MLFELBAE_1 | 587987 | 614058 | False | Siphoviridae | VOG0052, VOG10230, VOG0641, VOG7896, VOG3390, VOG4612, VOG4660, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG1886, VOG4581, VOG2703 |
| MLFELBAE_1 | 623760 | 626190 | False | Unknown | VOG4552, VOG0650, VOG9667 |
| MLFELBAE_4 | 49745 | 76455 | False | Myoviridae | VOG0136, VOG0753, VOG4705, VOG3390, VOG4612, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189 |
Phigaro v2.4.0 (Database: Jan. 2024 release)