safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
3	0	1	3.16

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
HNKIKHKM_14	165900	167819	tet(W)	ARO:3000194	tet(W)	96.71	100.00	AJ222769.3
HNKIKHKM_2	21837	22322	lnuA	ARO:3002835	lnuA	97.52	100.00	M14039.1
HNKIKHKM_2	7686	8171	lnuA	ARO:3002835	lnuA	97.52	100.00	M14039.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
HNKIKHKM_14	165900	167819	tet(W)	Doxycycline, Tetracycline, Minocycline	98.96	100.00	FN396364
HNKIKHKM_2	21837	22322	lnu(A)	Lincomycin	98.56	100.00	M14039

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
HNKIKHKM_2	21837	22319	lnu(A)	lincosamide nucleotidyltransferase Lnu(A)	EXACTX	100.00	100.00	WP_001829870.1
HNKIKHKM_2	7686	8168	lnu(A)	lincosamide nucleotidyltransferase Lnu(A)	EXACTX	100.00	100.00	WP_001829870.1
HNKIKHKM_14	165900	167816	tet(W)	tetracycline resistance ribosomal protection protein Tet(W)	BLASTX	99.53	100.00	WP_000691721.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
HNKIKHKM_3	673611	673805	CspA	5.27e-27	cold shock protein	83.08	98.48	CAA62903

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
HNKIKHKM_1	1.0	0.0	1.0
HNKIKHKM_2	1.0	0.0	1.0
HNKIKHKM_3	0.0	1.0	0.0
HNKIKHKM_4	1.0	0.0	1.0
HNKIKHKM_5	1.0	0.0	1.0
HNKIKHKM_6	1.0	0.0	1.0
HNKIKHKM_7	1.0	0.0	1.0
HNKIKHKM_8	0.0	1.0	0.0
HNKIKHKM_9	1.0	0.0	1.0
HNKIKHKM_10	0.0	1.0	0.0
HNKIKHKM_11	1.0	0.0	1.0
HNKIKHKM_12	1.0	0.0	1.0
HNKIKHKM_14	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
HNKIKHKM_3	738422	765207	True	Siphoviridae	VOG0221, VOG0221, VOG9502, VOG0641, VOG4589, VOG0204, VOG4553, VOG4573, VOG4556, VOG4544, VOG4571, VOG4581, VOG6154, VOG0198, VOG4548, VOG0327, VOG4606, VOG0026, VOG0021, VOG1047, VOG7438, VOG0979
HNKIKHKM_4	1740	21604	False	Siphoviridae	VOG1886, VOG0202, VOG4556, VOG4573, VOG9587, VOG4553, VOG0204, VOG0205, VOG0207, VOG4586, VOG0209, VOG0083, VOG0801, VOG3462, VOG10972, VOG0565, VOG9997, VOG4626
HNKIKHKM_5	2675	25095	False	Siphoviridae	VOG4841, VOG4581, VOG1886, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG0204, VOG0205, VOG0207, VOG4586, VOG0209, VOG0083, VOG0801, VOG3462, VOG10972, VOG4849, VOG0565, VOG9997, VOG4626
HNKIKHKM_8	42464	54703	False	Myoviridae / Siphoviridae	VOG0371, VOG0979, VOG5378, VOG0275, VOG9267, VOG9667, VOG4829, VOG4552, VOG2228, VOG7341, VOG1309, VOG0186, VOG4566, VOG0189
HNKIKHKM_9	5820	14827	False	Siphoviridae	VOG8520, VOG8520, VOG4552, VOG2228, VOG0321, VOG4548, VOG0322, VOG0052, VOG6545, VOG4632, VOG4609
HNKIKHKM_11	1632	20338	False	Siphoviridae	VOG10904, VOG0720, VOG4564, VOG2215, VOG1649, VOG4555, VOG4711, VOG4713, VOG0723, VOG5660, VOG0724, VOG0799, VOG0725, VOG0800, VOG4545, VOG0801, VOG3462

Phigaro v2.4.0 (Database: Jan. 2024 release)