safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	0	1	1.00

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
BMBIGOBI_1	52160	52864	WalR	2.23e-113	transcriptional regulatory protein	83.83	100.00	EPH95667

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
BMBIGOBI_1	0.0	1.0	0.0
BMBIGOBI_2	1.0	0.0	1.0
BMBIGOBI_3	1.0	0.0	1.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
BMBIGOBI_1	42492	56061	False	Siphoviridae	VOG0205, VOG4841, VOG4581, VOG4544, VOG2935, VOG4556, VOG4568, VOG0204, VOG0198, VOG6827
BMBIGOBI_1	1016990	1039345	False	Siphoviridae	VOG3643, VOG0198, VOG4841, VOG4581, VOG1886, VOG6939, VOG4556, VOG4568, VOG4553, VOG0204, VOG0205, VOG0207, VOG4586, VOG0209, VOG0660, VOG6163, VOG4605, VOG4599, VOG10972, VOG8870
BMBIGOBI_1	1189773	1265268	False	Siphoviridae	VOG8222, VOG0181, VOG0321, VOG4548, VOG9667, VOG0322, VOG6545, VOG4632, VOG4609, VOG0327, VOG0044, VOG4673, VOG1637, VOG6154, VOG4844, VOG4556, VOG4573, VOG4553, VOG0562, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG3498, VOG9997, VOG8917, VOG9888, VOG0602, VOG8294, VOG0275, VOG4602, VOG8520, VOG0787, VOG1638, VOG1563, VOG8222, VOG0181, VOG0321, VOG4548, VOG9667, VOG0322, VOG6545, VOG4632, VOG4632, VOG4609, VOG0327, VOG0044, VOG4673, VOG1637, VOG6154, VOG4844, VOG4556, VOG4573, VOG4553, VOG0562, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG3498, VOG9997, VOG8917, VOG9888, VOG0602, VOG8294
BMBIGOBI_1	2195161	2221982	False	Siphoviridae	VOG1637, VOG8294, VOG3498, VOG10608, VOG1335, VOG4545, VOG1333, VOG4634, VOG0704, VOG1330, VOG4713, VOG1887, VOG3434, VOG10914, VOG4564, VOG7038, VOG0720, VOG4544, VOG0796, VOG9295
BMBIGOBI_1	2226558	2234941	False	Siphoviridae	VOG4566, VOG0186, VOG1309, VOG7298, VOG0181, VOG0513, VOG6495, VOG11003, VOG11077, VOG9667, VOG4602
BMBIGOBI_1	2924839	2950450	False	Siphoviridae	VOG0254, VOG8870, VOG10957, VOG4599, VOG4605, VOG0660, VOG4586, VOG0207, VOG0205, VOG0204, VOG4553, VOG4573, VOG4556, VOG0202, VOG1886, VOG4581, VOG7810, VOG0741, VOG4841, VOG0198, VOG1049, VOG0634, VOG0044, VOG10015