Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 4 | 16.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| NGNMMNLO_1 | 2561884 | 2562738 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| NGNMMNLO_1 | 2543130 | 2544332 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
| NGNMMNLO_1 | 2322692 | 2323861 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| NGNMMNLO_1 | 1339607 | 1339924 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| NGNMMNLO_1 | 1339269 | 1339607 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| NGNMMNLO_1 | 873917 | 876487 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| NGNMMNLO_1 | 582546 | 584189 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
| NGNMMNLO_1 | 330647 | 331240 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| NGNMMNLO_1 | 280126 | 281559 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| NGNMMNLO_1 | 267313 | 268233 | mphK | ARO:3004541 | mphK | 99.02 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| NGNMMNLO_1 | 267313 | 268233 | mph(K) | Spiramycin, Telithromycin | 99.35 | 100.00 | NC_000964 |
| NGNMMNLO_1 | 2561884 | 2562738 | aadK | Streptomycin | 98.71 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| NGNMMNLO_1 | 267313 | 268230 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.02 | 100.00 | WP_003246254.1 |
| NGNMMNLO_1 | 2561887 | 2562738 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| NGNMMNLO_1 | 582549 | 584189 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| NGNMMNLO_1 | 4083911 | 4084429 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.64 | 100.00 | WP_003242546.1 |
| NGNMMNLO_1 | 2027182 | 2029779 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.65 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| NGNMMNLO_1 | 3590822 | 3592003 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| NGNMMNLO_1 | 3165881 | 3166666 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| NGNMMNLO_1 | 2125381 | 2126022 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| NGNMMNLO_1 | 3164659 | 3165855 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| NGNMMNLO_1 | 1972939 | 1974702 | dep/capD | 0.0 | Capsule | Immune modulation | 99.32 | 100.00 | NP_389723 |
| NGNMMNLO_1 | 3590358 | 3590807 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| NGNMMNLO_1 | 3589197 | 3590339 | capA | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | NP_391469 |
| NGNMMNLO_1 | 3154889 | 3162025 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.72 | 100.00 | NP_391076 |
| NGNMMNLO_1 | 3162045 | 3162983 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.40 | 100.00 | NP_391077 |
| NGNMMNLO_1 | 3163011 | 3164630 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.41 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| NGNMMNLO_1 | 132341 | 133477 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| NGNMMNLO_1 | 18601 | 19470 | pdxS | 3.06e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| NGNMMNLO_1 | 1651024 | 1651800 | CodY | 3.34e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| NGNMMNLO_1 | 3429468 | 3430037 | ClpP | 2.47e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| NGNMMNLO_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| NGNMMNLO_1 | 1283467 | 1309404 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| NGNMMNLO_1 | 2493532 | 2523568 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600 |
| NGNMMNLO_1 | 2740792 | 2763740 | False | Myoviridae | VOG4574, VOG0641, VOG4705, VOG3390, VOG7718, VOG4845, VOG4691, VOG5205, VOG0573, VOG1348, VOG1433, VOG1956, VOG6163, VOG4699, VOG1352, VOG1883, VOG1881, VOG1353, VOG4623, VOG4564, VOG0815, VOG4553, VOG4555, VOG0720 |
Phigaro v2.4.0 (Database: Jan. 2024 release)