Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 3 | 16 | 35 | 38.60 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KAJADNOI_34 | 2202 | 2939 | ErmB | ARO:3000375 | ErmB | 98.37 | 98.79 | AF242872.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KAJADNOI_34 | 2202 | 2939 | erm(B) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 100.00 | JN899585 |
| KAJADNOI_41 | 1107 | 2324 | mef(A) | Erythromycin, Azithromycin | 100.00 | 100.00 | U83667 |
| KAJADNOI_41 | 2444 | 3513 | msr(D) | Erythromycin, Azithromycin, Telithromycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 73.09 | AF274302 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KAJADNOI_34 | 2202 | 2936 | erm(B) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) | EXACTX | 100.00 | 100.00 | WP_002321849.1 |
| KAJADNOI_41 | 1107 | 2321 | mef(A) | macrolide efflux MFS transporter Mef(A) | EXACTX | 100.00 | 100.00 | WP_000417519.1 |
| KAJADNOI_41 | 2444 | 3511 | msr(D) | ABC-F type ribosomal protection protein Msr(D) | PARTIAL_CONTIG_ENDX | 100.00 | 73.10 | WP_000420313.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KAJADNOI_5 | 24090 | 24941 | rfbD | 0.0 | Capsule | Immune modulation | 94.95 | 100.00 | WP_014621820 |
| KAJADNOI_2 | 80168 | 81535 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.01 | 100.00 | WP_011226045 |
| KAJADNOI_2 | 76469 | 77929 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 93.98 | 100.00 | WP_011681177 |
| KAJADNOI_2 | 76469 | 77929 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 93.84 | 100.14 | WP_011226049 |
| KAJADNOI_2 | 80168 | 81533 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 93.70 | 99.85 | WP_011681173 |
| KAJADNOI_2 | 79372 | 80112 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 93.52 | 100.00 | WP_011681174 |
| KAJADNOI_2 | 76469 | 77929 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 93.22 | 100.14 | WP_041827043 |
| KAJADNOI_2 | 79372 | 80112 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 92.98 | 100.00 | WP_011226046 |
| KAJADNOI_2 | 79372 | 80107 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 92.53 | 98.13 | WP_011227246 |
| KAJADNOI_2 | 77930 | 78661 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 92.35 | 100.00 | WP_011226048 |
| KAJADNOI_2 | 77930 | 78661 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 91.67 | 100.00 | WP_011681176 |
| KAJADNOI_1 | 148237 | 148830 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 91.41 | 100.00 | WP_011226149 |
| KAJADNOI_2 | 77930 | 78661 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 91.39 | 100.00 | WP_011227248 |
| KAJADNOI_1 | 147368 | 148237 | rfbA | 0.0 | Capsule | Immune modulation | 91.38 | 100.00 | WP_011681286 |
| KAJADNOI_1 | 148237 | 148830 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 91.25 | 100.00 | WP_011226149 |
| KAJADNOI_1 | 148237 | 148830 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 91.25 | 100.00 | WP_011226149 |
| KAJADNOI_23 | 17393 | 18232 | cbpD | 0.0 | Autolysin | Exoenzyme | 91.07 | 100.00 | WP_011226733 |
| KAJADNOI_2 | 184894 | 186552 | fbp54 | 0.0 | FBPs | Adherence | 90.84 | 99.94 | WP_011227179 |
| KAJADNOI_2 | 78670 | 79362 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 88.62 | 99.86 | WP_011227247 |
| KAJADNOI_2 | 78670 | 79362 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 88.62 | 99.86 | WP_011226047 |
| KAJADNOI_6 | 33200 | 34503 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.17 | 99.54 | WP_000022832 |
| KAJADNOI_26 | 6563 | 7573 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.46 | 99.80 | WP_002986042 |
| KAJADNOI_2 | 78670 | 79362 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 86.89 | 99.86 | WP_011681175 |
| KAJADNOI_11 | 17754 | 18515 | srtA | 0.0 | Sortase A | Exoenzyme | 84.67 | 99.87 | - |
| KAJADNOI_1 | 149216 | 150262 | rfbB | 0.0 | Capsule | Immune modulation | 81.51 | 99.90 | WP_009659423 |
| KAJADNOI_13 | 1810 | 3093 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.68 | 99.61 | WP_000107753 |
| KAJADNOI_2 | 90692 | 91277 | SPD_RS01775 | 2.23e-140 | Capsule | Immune modulation | 82.28 | 96.53 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KAJADNOI_11 | 15064 | 14081 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.95 | 100.00 | AAK75326 |
| KAJADNOI_11 | 26648 | 27721 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| KAJADNOI_4 | 12591 | 10285 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.54 | 99.22 | AAK74619 |
| KAJADNOI_11 | 45705 | 47951 | metE | 0.0 | methionine biosynthesis enzymes | 93.59 | 100.00 | AAK74738 |
| KAJADNOI_15 | 43042 | 44010 | spr0084 | 0.0 | pathogenitcity related | 93.50 | 98.48 | AAK98888 |
| KAJADNOI_7 | 68732 | 67251 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.32 | 100.00 | ABJ54480 |
| KAJADNOI_37 | 1113 | 142 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.36 | 100.00 | ABF36968 |
| KAJADNOI_1 | 121990 | 123720 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| KAJADNOI_4 | 157219 | 158370 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| KAJADNOI_5 | 10358 | 12346 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.44 | 100.00 | AE014259_2 |
| KAJADNOI_10 | 79105 | 77627 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| KAJADNOI_6 | 34503 | 33202 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| KAJADNOI_10 | 63747 | 61855 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.58 | 98.90 | ABP91129 |
| KAJADNOI_11 | 50873 | 49164 | PGM | 0.0 | phosphoglucomutase | 84.76 | 99.65 | ACH87041 |
| KAJADNOI_40 | 2879 | 3970 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| KAJADNOI_27 | 1992 | 982 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| KAJADNOI_2 | 171387 | 172748 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.89 | 99.56 | ABP90420 |
| KAJADNOI_6 | 48909 | 48019 | bglB | 2.65e-178 | putative phospho--glucosidase | 87.54 | 99.66 | AAK33487 |
| KAJADNOI_11 | 90240 | 89290 | SP_0676 | 2.85e-170 | transcription facot | 81.39 | 98.45 | AAK74821 |
| KAJADNOI_11 | 48141 | 48998 | metF | 4.25e-169 | methionine biosynthesis enzymes | 87.06 | 99.31 | AAK74739 |
| KAJADNOI_9 | 39530 | 40210 | NisR | 5.25e-133 | two-component signal-transduction systems | 94.71 | 100.00 | ABP89873 |
| KAJADNOI_9 | 40198 | 40902 | NisK | 2.27e-131 | two-component signal-transduction systems | 91.06 | 100.00 | ABP89872 |
| KAJADNOI_20 | 36143 | 35472 | rr01 | 7.30e-128 | response regulator | 84.82 | 99.56 | CAB54566 |
| KAJADNOI_2 | 31665 | 32369 | covR | 1.11e-127 | responder protein | 85.96 | 100.00 | AAK71319 |
| KAJADNOI_11 | 54841 | 55512 | ciaR | 7.05e-127 | two component system response regulator | 86.61 | 99.12 | AIK75708 |
| KAJADNOI_11 | 54841 | 55503 | CiaR | 2.80e-125 | two-component response regulator | 88.23 | 98.66 | AAK74935 |
| KAJADNOI_2 | 31665 | 32369 | walR | 2.02e-120 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| KAJADNOI_8 | 55590 | 56276 | CovR | 2.42e-120 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| KAJADNOI_40 | 4143 | 4538 | SpxA2 | 4.58e-77 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| KAJADNOI_2 | 159922 | 159521 | SpxA1 | 4.59e-70 | transcriptional regulator | 82.84 | 100.00 | ERL19273 |
| KAJADNOI_28 | 9980 | 10393 | GrpE | 1.23e-68 | protein grpE | 81.88 | 80.59 | ABP89267 |
| KAJADNOI_8 | 78798 | 79085 | spr0479 | 2.35e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| KAJADNOI_3 | 42421 | 42167 | spr1327 | 8.45e-45 | essential gene | 92.94 | 100.00 | AAL00131 |
| KAJADNOI_21 | 28448 | 28711 | spr0175 | 1.86e-43 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| KAJADNOI_21 | 29273 | 29539 | spr0177 | 4.12e-42 | essential gene | 83.15 | 88.12 | AAK98981 |
| KAJADNOI_13 | 3260 | 3751 | RopE (M5005_Spy_1611) | 4.54e-73 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| KAJADNOI_4 | 12591 | 10291 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.22 | 98.08 | MYN69560 |
| KAJADNOI_14 | 31166 | 32185 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.35 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KAJADNOI_1 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_2 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_3 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_4 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_5 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_6 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_7 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_8 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_9 | 1.0 | 0.0 | 1.0 |
| KAJADNOI_10 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_11 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_12 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_13 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_14 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_15 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_16 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_17 | 1.0 | 0.0 | 1.0 |
| KAJADNOI_18 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_19 | 1.0 | 0.0 | 1.0 |
| KAJADNOI_20 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_21 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_22 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_23 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_24 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_25 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_26 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_27 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_28 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_29 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_30 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_31 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_32 | 1.0 | 0.0 | 1.0 |
| KAJADNOI_33 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_34 | 1.0 | 0.0 | 1.0 |
| KAJADNOI_35 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_36 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_37 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_38 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_39 | 1.0 | 0.0 | 1.0 |
| KAJADNOI_40 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_41 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_42 | 1.0 | 0.0 | 1.0 |
| KAJADNOI_43 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_44 | 1.0 | 0.0 | 1.0 |
| KAJADNOI_46 | 1.0 | 0.0 | 1.0 |
| KAJADNOI_48 | 0.0 | 1.0 | 0.0 |
| KAJADNOI_49 | 1.0 | 0.0 | 1.0 |
| KAJADNOI_50 | 1.0 | 0.0 | 1.0 |
| KAJADNOI_51 | 1.0 | 0.0 | 1.0 |
| KAJADNOI_52 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)