Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 17 | 34 | 38.01 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BJHFPPGC_1 | 489061 | 490521 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.40 | 100.00 | WP_011681177 |
| BJHFPPGC_1 | 489061 | 490521 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.92 | 100.21 | WP_011226049 |
| BJHFPPGC_2 | 85945 | 86661 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 96.79 | 100.00 | WP_011681442 |
| BJHFPPGC_1 | 489061 | 490521 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.37 | 100.21 | WP_041827043 |
| BJHFPPGC_2 | 90806 | 91657 | rfbD | 0.0 | Capsule | Immune modulation | 93.90 | 100.00 | WP_014621820 |
| BJHFPPGC_1 | 490522 | 491253 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 93.85 | 100.00 | WP_011681176 |
| BJHFPPGC_1 | 490522 | 491253 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 93.72 | 100.00 | WP_011226048 |
| BJHFPPGC_1 | 491964 | 492704 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 93.39 | 100.00 | WP_011681174 |
| BJHFPPGC_1 | 492761 | 494126 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 93.27 | 99.85 | WP_011681173 |
| BJHFPPGC_1 | 490522 | 491253 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 93.03 | 100.00 | WP_011227248 |
| BJHFPPGC_2 | 84829 | 85599 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 92.88 | 99.10 | WP_011681440 |
| BJHFPPGC_1 | 491964 | 492704 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 92.71 | 100.00 | WP_011226046 |
| BJHFPPGC_1 | 492761 | 494128 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 92.62 | 100.00 | WP_011226045 |
| BJHFPPGC_1 | 491964 | 492699 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 92.53 | 98.13 | WP_011227246 |
| BJHFPPGC_1 | 323251 | 323844 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 92.26 | 100.00 | WP_011226149 |
| BJHFPPGC_1 | 323251 | 323844 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 92.09 | 100.00 | WP_011226149 |
| BJHFPPGC_1 | 323251 | 323844 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 92.09 | 100.00 | WP_011226149 |
| BJHFPPGC_1 | 322382 | 323251 | rfbA | 0.0 | Capsule | Immune modulation | 91.15 | 100.00 | WP_011681286 |
| BJHFPPGC_12 | 18802 | 19641 | cbpD | 0.0 | Autolysin | Exoenzyme | 91.07 | 100.00 | WP_011226733 |
| BJHFPPGC_3 | 22785 | 24443 | fbp54 | 0.0 | FBPs | Adherence | 90.66 | 99.94 | WP_011227179 |
| BJHFPPGC_6 | 14438 | 15448 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 88.75 | 99.80 | WP_002986042 |
| BJHFPPGC_1 | 122395 | 123698 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.40 | 99.54 | WP_000022832 |
| BJHFPPGC_3 | 282413 | 283174 | srtA | 0.0 | Sortase A | Exoenzyme | 84.53 | 99.87 | - |
| BJHFPPGC_1 | 491262 | 491954 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 84.01 | 99.86 | WP_011681175 |
| BJHFPPGC_4 | 2635 | 3918 | tig/ropA | 0.0 | Trigger factor | Stress survival | 81.01 | 99.53 | WP_000107753 |
| BJHFPPGC_1 | 324046 | 325092 | rfbB | 0.0 | Capsule | Immune modulation | 80.13 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BJHFPPGC_3 | 279723 | 278740 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.65 | 100.00 | AAK75326 |
| BJHFPPGC_3 | 291312 | 292385 | MntE | 0.0 | manganese homeostasis | 95.53 | 99.72 | ABF32167 |
| BJHFPPGC_2 | 300217 | 302523 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.41 | 99.22 | AAK74619 |
| BJHFPPGC_7 | 63931 | 62450 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.32 | 100.00 | ABJ54480 |
| BJHFPPGC_3 | 310365 | 312611 | metE | 0.0 | methionine biosynthesis enzymes | 93.19 | 100.00 | AAK74738 |
| BJHFPPGC_1 | 33486 | 34454 | spr0084 | 0.0 | pathogenitcity related | 92.26 | 98.48 | AAK98888 |
| BJHFPPGC_12 | 4385 | 5356 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.36 | 100.00 | ABF36968 |
| BJHFPPGC_3 | 232922 | 239470 | PrtA | 0.0 | serine protease | 90.35 | 101.73 | ABJ55268 |
| BJHFPPGC_1 | 297002 | 298732 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| BJHFPPGC_2 | 133150 | 131999 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| BJHFPPGC_2 | 104906 | 102924 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.56 | 99.70 | AE014259_2 |
| BJHFPPGC_5 | 25902 | 27380 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| BJHFPPGC_1 | 122395 | 123696 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| BJHFPPGC_5 | 41245 | 43137 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.42 | 98.90 | ABP91129 |
| BJHFPPGC_3 | 315529 | 313820 | PGM | 0.0 | phosphoglucomutase | 84.76 | 99.65 | ACH87041 |
| BJHFPPGC_12 | 38184 | 39275 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| BJHFPPGC_5 | 155754 | 154744 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| BJHFPPGC_1 | 592490 | 593851 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.46 | 99.56 | ABP90420 |
| BJHFPPGC_1 | 107985 | 108875 | bglB | 7.40e-178 | putative phospho--glucosidase | 87.88 | 99.66 | AAK33487 |
| BJHFPPGC_3 | 312801 | 313658 | metF | 1.07e-169 | methionine biosynthesis enzymes | 87.76 | 99.31 | AAK74739 |
| BJHFPPGC_3 | 116491 | 117441 | SP_0676 | 4.47e-169 | transcription facot | 81.07 | 98.45 | AAK74821 |
| BJHFPPGC_3 | 195713 | 196384 | rr01 | 6.16e-127 | response regulator | 84.82 | 99.56 | CAB54566 |
| BJHFPPGC_1 | 443280 | 443984 | covR | 9.42e-126 | responder protein | 85.11 | 100.00 | AAK71319 |
| BJHFPPGC_3 | 320195 | 320866 | ciaR | 3.09e-125 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| BJHFPPGC_3 | 320195 | 320863 | CiaR | 5.71e-125 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| BJHFPPGC_4 | 157946 | 158632 | CovR | 4.65e-120 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| BJHFPPGC_1 | 443280 | 443984 | walR | 1.73e-119 | DNA-binding response regulator | 81.70 | 100.00 | ANC99504 |
| BJHFPPGC_12 | 39451 | 39846 | SpxA2 | 3.94e-76 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| BJHFPPGC_1 | 581022 | 580621 | SpxA1 | 9.06e-69 | transcriptional regulator | 82.09 | 100.00 | ERL19273 |
| BJHFPPGC_13 | 28060 | 28473 | GrpE | 6.30e-68 | protein grpE | 81.16 | 80.59 | ABP89267 |
| BJHFPPGC_4 | 181160 | 181447 | spr0479 | 5.52e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| BJHFPPGC_9 | 4893 | 4639 | spr1327 | 2.18e-45 | essential gene | 94.12 | 100.00 | AAL00131 |
| BJHFPPGC_5 | 72423 | 72160 | spr0175 | 9.53e-43 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| BJHFPPGC_5 | 71597 | 71331 | spr0177 | 1.33e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| BJHFPPGC_4 | 4085 | 4576 | RopE (M5005_Spy_1611) | 1.03e-72 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| BJHFPPGC_2 | 300217 | 302517 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.09 | 98.08 | MYN69560 |
| BJHFPPGC_5 | 119310 | 120329 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.35 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BJHFPPGC_1 | 0.0 | 1.0 | 0.0 |
| BJHFPPGC_2 | 0.0 | 1.0 | 0.0 |
| BJHFPPGC_3 | 0.0 | 1.0 | 0.0 |
| BJHFPPGC_4 | 0.0 | 1.0 | 0.0 |
| BJHFPPGC_5 | 0.0 | 1.0 | 0.0 |
| BJHFPPGC_6 | 0.0 | 1.0 | 0.0 |
| BJHFPPGC_7 | 0.0 | 1.0 | 0.0 |
| BJHFPPGC_8 | 0.0 | 1.0 | 0.0 |
| BJHFPPGC_9 | 0.0 | 1.0 | 0.0 |
| BJHFPPGC_10 | 0.0 | 1.0 | 0.0 |
| BJHFPPGC_11 | 0.0 | 1.0 | 0.0 |
| BJHFPPGC_12 | 0.0 | 1.0 | 0.0 |
| BJHFPPGC_13 | 0.0 | 1.0 | 0.0 |
| BJHFPPGC_14 | 0.0 | 1.0 | 0.0 |
| BJHFPPGC_15 | 1.0 | 0.0 | 1.0 |
| BJHFPPGC_18 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BJHFPPGC_1 | 125053 | 161121 | False | Siphoviridae | VOG3304, VOG4602, VOG6127, VOG4771, VOG4552, VOG3478, VOG0651, VOG4566, VOG0189, VOG3430, VOG1309, VOG0186, VOG0226, VOG4693, VOG0655, VOG3468, VOG0656, VOG0152, VOG4567, VOG0657, VOG0658, VOG0198, VOG0796, VOG10227, VOG0720, VOG4564, VOG5041, VOG4553, VOG3649, VOG4713, VOG0723, VOG9583, VOG0724, VOG0799, VOG0725, VOG0800, VOG9762, VOG0727, VOG4763, VOG4684, VOG0641, VOG0054, VOG5034, VOG7557, VOG4025 |
Phigaro v2.4.0 (Database: Jan. 2024 release)