Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 20 | 34 | 39.50 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KMKPCFBF_1 | 300568 | 301785 | mef(A) | Erythromycin, Azithromycin | 100.00 | 100.00 | U83667 |
| KMKPCFBF_1 | 301905 | 303368 | msr(D) | Erythromycin, Azithromycin, Telithromycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 100.00 | AF274302 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KMKPCFBF_1 | 300568 | 301782 | mef(A) | macrolide efflux MFS transporter Mef(A) | EXACTX | 100.00 | 100.00 | WP_000417519.1 |
| KMKPCFBF_1 | 301905 | 303365 | msr(D) | ABC-F type ribosomal protection protein Msr(D) | EXACTX | 100.00 | 100.00 | WP_000420313.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KMKPCFBF_2 | 211814 | 212529 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 96.51 | 99.86 | WP_011681442 |
| KMKPCFBF_2 | 208624 | 209877 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 95.14 | 99.44 | WP_011681445 |
| KMKPCFBF_2 | 207654 | 208505 | rfbD | 0.0 | Capsule | Immune modulation | 94.95 | 100.00 | WP_014621820 |
| KMKPCFBF_2 | 210844 | 211812 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 94.84 | 100.00 | WP_011681443 |
| KMKPCFBF_1 | 648038 | 649403 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.14 | 99.85 | WP_011681173 |
| KMKPCFBF_1 | 644337 | 645797 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 94.05 | 100.07 | WP_011226049 |
| KMKPCFBF_1 | 647240 | 647980 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 93.93 | 100.00 | WP_011681174 |
| KMKPCFBF_1 | 644337 | 645797 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 93.84 | 100.00 | WP_011681177 |
| KMKPCFBF_1 | 648038 | 649405 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 93.79 | 100.00 | WP_011226045 |
| KMKPCFBF_2 | 212875 | 213645 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 93.26 | 99.23 | WP_011681440 |
| KMKPCFBF_1 | 644337 | 645797 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 93.16 | 100.07 | WP_041827043 |
| KMKPCFBF_1 | 647240 | 647980 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 93.12 | 100.00 | WP_011226046 |
| KMKPCFBF_1 | 647240 | 647975 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 92.66 | 98.13 | WP_011227246 |
| KMKPCFBF_1 | 645798 | 646529 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 91.94 | 100.00 | WP_011226048 |
| KMKPCFBF_1 | 645798 | 646529 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 91.53 | 100.00 | WP_011681176 |
| KMKPCFBF_1 | 472763 | 473356 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 91.41 | 100.00 | WP_011226149 |
| KMKPCFBF_1 | 472763 | 473356 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 91.25 | 100.00 | WP_011226149 |
| KMKPCFBF_1 | 472763 | 473356 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 91.25 | 100.00 | WP_011226149 |
| KMKPCFBF_1 | 471894 | 472763 | rfbA | 0.0 | Capsule | Immune modulation | 91.03 | 100.00 | WP_011681286 |
| KMKPCFBF_1 | 645798 | 646529 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 90.98 | 100.00 | WP_011227248 |
| KMKPCFBF_1 | 754894 | 756552 | fbp54 | 0.0 | FBPs | Adherence | 90.66 | 99.94 | WP_011227179 |
| KMKPCFBF_7 | 14853 | 15863 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 88.45 | 99.80 | WP_002986042 |
| KMKPCFBF_1 | 272464 | 273767 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.24 | 99.54 | WP_000022832 |
| KMKPCFBF_1 | 646538 | 647230 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 88.04 | 99.86 | WP_011227247 |
| KMKPCFBF_1 | 646538 | 647230 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 88.04 | 99.86 | WP_011226047 |
| KMKPCFBF_1 | 646538 | 647230 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 87.46 | 99.86 | WP_011681175 |
| KMKPCFBF_1 | 91625 | 92521 | cbpD | 0.0 | Autolysin | Exoenzyme | 84.66 | 93.19 | WP_082302295 |
| KMKPCFBF_1 | 473622 | 474668 | rfbB | 0.0 | Capsule | Immune modulation | 84.35 | 99.90 | WP_002263087 |
| KMKPCFBF_6 | 79968 | 80729 | srtA | 0.0 | Sortase A | Exoenzyme | 83.90 | 99.74 | - |
| KMKPCFBF_10 | 1837 | 3120 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.61 | 99.61 | WP_000107753 |
| KMKPCFBF_1 | 657556 | 658139 | SPD_RS01775 | 4.71e-157 | Capsule | Immune modulation | 84.10 | 96.20 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KMKPCFBF_6 | 83417 | 84400 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.04 | 100.00 | AAK75326 |
| KMKPCFBF_6 | 71837 | 70764 | MntE | 0.0 | manganese homeostasis | 95.25 | 99.72 | ABF32167 |
| KMKPCFBF_2 | 31165 | 28859 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.54 | 99.22 | AAK74619 |
| KMKPCFBF_4 | 74551 | 76032 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.12 | 100.00 | ABJ54480 |
| KMKPCFBF_1 | 180287 | 181255 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| KMKPCFBF_6 | 52784 | 50538 | metE | 0.0 | methionine biosynthesis enzymes | 92.39 | 100.00 | AAK74738 |
| KMKPCFBF_9 | 4366 | 5337 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| KMKPCFBF_6 | 141144 | 134671 | PrtA | 0.0 | serine protease | 91.35 | 100.61 | ABJ55268 |
| KMKPCFBF_1 | 446509 | 448239 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| KMKPCFBF_2 | 193791 | 195779 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.59 | 100.00 | AE014259_2 |
| KMKPCFBF_2 | 165439 | 166590 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| KMKPCFBF_3 | 25881 | 27359 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| KMKPCFBF_1 | 272464 | 273765 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| KMKPCFBF_3 | 41239 | 43131 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.58 | 98.90 | ABP91129 |
| KMKPCFBF_6 | 47619 | 49328 | PGM | 0.0 | phosphoglucomutase | 85.11 | 99.65 | ACH87041 |
| KMKPCFBF_9 | 44416 | 45507 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| KMKPCFBF_3 | 185762 | 184752 | pdh | 0.0 | pyruvate dehydrogenase | 83.38 | 99.70 | ABP91006 |
| KMKPCFBF_1 | 739331 | 740692 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.46 | 99.56 | ABP90420 |
| KMKPCFBF_1 | 258045 | 258935 | bglB | 8.77e-177 | putative phospho--glucosidase | 87.20 | 99.66 | AAK33487 |
| KMKPCFBF_6 | 8355 | 9305 | SP_0676 | 2.41e-170 | transcription facot | 81.39 | 98.45 | AAK74821 |
| KMKPCFBF_6 | 50351 | 49494 | metF | 3.82e-169 | methionine biosynthesis enzymes | 87.76 | 99.31 | AAK74739 |
| KMKPCFBF_1 | 598656 | 599360 | covR | 3.60e-126 | responder protein | 85.53 | 100.00 | AAK71319 |
| KMKPCFBF_6 | 43649 | 42978 | ciaR | 1.45e-125 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| KMKPCFBF_4 | 14018 | 13347 | rr01 | 1.53e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| KMKPCFBF_6 | 43649 | 42981 | CiaR | 2.68e-125 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| KMKPCFBF_5 | 149719 | 150405 | CovR | 4.44e-120 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| KMKPCFBF_1 | 598656 | 599360 | walR | 8.19e-120 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| KMKPCFBF_9 | 45681 | 46076 | SpxA2 | 4.55e-76 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| KMKPCFBF_1 | 727864 | 727463 | SpxA1 | 8.94e-69 | transcriptional regulator | 82.09 | 100.00 | ERL19273 |
| KMKPCFBF_8 | 48398 | 48811 | GrpE | 4.27e-68 | protein grpE | 81.88 | 80.59 | ABP89267 |
| KMKPCFBF_5 | 172930 | 173217 | spr0479 | 5.38e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| KMKPCFBF_1 | 146785 | 147039 | spr1327 | 1.31e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| KMKPCFBF_3 | 94245 | 93982 | spr0175 | 1.11e-42 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| KMKPCFBF_3 | 93421 | 93155 | spr0177 | 2.46e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| KMKPCFBF_10 | 3287 | 3778 | RopE (M5005_Spy_1611) | 5.43e-74 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| KMKPCFBF_2 | 31165 | 28865 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.36 | 98.08 | MYN69560 |
| KMKPCFBF_3 | 149351 | 150370 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.35 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KMKPCFBF_1 | 0.0 | 1.0 | 0.0 |
| KMKPCFBF_2 | 0.0 | 1.0 | 0.0 |
| KMKPCFBF_3 | 0.0 | 1.0 | 0.0 |
| KMKPCFBF_4 | 0.0 | 1.0 | 0.0 |
| KMKPCFBF_5 | 0.0 | 1.0 | 0.0 |
| KMKPCFBF_6 | 0.0 | 1.0 | 0.0 |
| KMKPCFBF_7 | 0.0 | 1.0 | 0.0 |
| KMKPCFBF_8 | 0.0 | 1.0 | 0.0 |
| KMKPCFBF_9 | 0.0 | 1.0 | 0.0 |
| KMKPCFBF_10 | 0.0 | 1.0 | 0.0 |
| KMKPCFBF_11 | 1.0 | 0.0 | 1.0 |
| KMKPCFBF_13 | 0.0 | 1.0 | 0.0 |
| KMKPCFBF_14 | 0.0 | 1.0 | 0.0 |
| KMKPCFBF_15 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)