Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 15 | 33 | 36.25 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| JAKIAOLI_3 | 315192 | 316043 | rfbD | 0.0 | Capsule | Immune modulation | 95.07 | 100.00 | WP_014621820 |
| JAKIAOLI_2 | 48101 | 49561 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 94.32 | 100.14 | WP_011226049 |
| JAKIAOLI_2 | 48101 | 49561 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 94.18 | 100.00 | WP_011681177 |
| JAKIAOLI_2 | 48101 | 49561 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 93.43 | 100.14 | WP_041827043 |
| JAKIAOLI_2 | 51802 | 53169 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 93.13 | 100.00 | WP_011226045 |
| JAKIAOLI_2 | 51802 | 53167 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 92.97 | 99.85 | WP_011681173 |
| JAKIAOLI_2 | 49562 | 50293 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 91.67 | 100.00 | WP_011226048 |
| JAKIAOLI_2 | 49562 | 50293 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 91.26 | 100.00 | WP_011681176 |
| JAKIAOLI_1 | 440971 | 441564 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 91.25 | 100.00 | WP_011226149 |
| JAKIAOLI_1 | 440102 | 440971 | rfbA | 0.0 | Capsule | Immune modulation | 91.15 | 100.00 | WP_011681286 |
| JAKIAOLI_2 | 156150 | 157808 | fbp54 | 0.0 | FBPs | Adherence | 91.14 | 99.94 | WP_011227179 |
| JAKIAOLI_1 | 440971 | 441564 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 91.08 | 100.00 | WP_011226149 |
| JAKIAOLI_1 | 440971 | 441564 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 91.08 | 100.00 | WP_011226149 |
| JAKIAOLI_2 | 51004 | 51744 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 90.84 | 99.87 | WP_011681174 |
| JAKIAOLI_2 | 49562 | 50293 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 90.71 | 100.00 | WP_011227248 |
| JAKIAOLI_2 | 51004 | 51744 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 89.89 | 99.87 | WP_011226046 |
| JAKIAOLI_2 | 51004 | 51739 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 89.55 | 98.00 | WP_011227246 |
| JAKIAOLI_6 | 15163 | 16068 | cbpD | 0.0 | Autolysin | Exoenzyme | 89.07 | 93.50 | WP_082302295 |
| JAKIAOLI_3 | 13439 | 14449 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 88.55 | 99.80 | WP_002986042 |
| JAKIAOLI_1 | 215480 | 216783 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.24 | 99.54 | WP_000022832 |
| JAKIAOLI_2 | 50302 | 50994 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 85.45 | 99.86 | WP_011226047 |
| JAKIAOLI_2 | 50302 | 50994 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 85.16 | 99.86 | WP_011227247 |
| JAKIAOLI_2 | 50302 | 50994 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 84.87 | 99.86 | WP_011681175 |
| JAKIAOLI_2 | 307618 | 308379 | srtA | 0.0 | Sortase A | Exoenzyme | 84.27 | 99.87 | - |
| JAKIAOLI_1 | 441836 | 442882 | rfbB | 0.0 | Capsule | Immune modulation | 80.80 | 100.00 | WP_009659423 |
| JAKIAOLI_5 | 1863 | 3146 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.61 | 99.61 | WP_000107753 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| JAKIAOLI_2 | 311069 | 312052 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.95 | 100.00 | AAK75326 |
| JAKIAOLI_2 | 299486 | 298413 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| JAKIAOLI_3 | 136972 | 134666 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.41 | 99.22 | AAK74619 |
| JAKIAOLI_2 | 445557 | 447038 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.12 | 100.00 | ABJ54480 |
| JAKIAOLI_2 | 280429 | 278183 | metE | 0.0 | methionine biosynthesis enzymes | 93.06 | 100.00 | AAK74738 |
| JAKIAOLI_1 | 126488 | 127456 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| JAKIAOLI_8 | 39317 | 38346 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.36 | 100.00 | ABF36968 |
| JAKIAOLI_1 | 414720 | 416450 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 90.12 | 100.00 | AAK34199 |
| JAKIAOLI_3 | 273589 | 274740 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| JAKIAOLI_3 | 301936 | 303924 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.44 | 100.00 | AE014259_2 |
| JAKIAOLI_4 | 171052 | 169574 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| JAKIAOLI_1 | 215480 | 216781 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| JAKIAOLI_4 | 155719 | 153827 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.58 | 98.90 | ABP91129 |
| JAKIAOLI_2 | 275263 | 276972 | PGM | 0.0 | phosphoglucomutase | 84.94 | 99.65 | ACH87041 |
| JAKIAOLI_8 | 2023 | 932 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| JAKIAOLI_4 | 27300 | 28310 | pdh | 0.0 | pyruvate dehydrogenase | 83.38 | 99.70 | ABP91006 |
| JAKIAOLI_2 | 142384 | 143745 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.67 | 99.56 | ABP90420 |
| JAKIAOLI_1 | 201065 | 201955 | bglB | 6.12e-177 | putative phospho--glucosidase | 87.20 | 99.66 | AAK33487 |
| JAKIAOLI_2 | 232484 | 233434 | SP_0676 | 6.25e-170 | transcription facot | 81.39 | 98.45 | AAK74821 |
| JAKIAOLI_2 | 277994 | 277137 | metF | 7.56e-170 | methionine biosynthesis enzymes | 88.11 | 99.31 | AAK74739 |
| JAKIAOLI_2 | 389691 | 389020 | rr01 | 1.49e-126 | response regulator | 84.38 | 99.56 | CAB54566 |
| JAKIAOLI_2 | 2422 | 3126 | covR | 2.25e-126 | responder protein | 85.53 | 100.00 | AAK71319 |
| JAKIAOLI_2 | 271296 | 270625 | ciaR | 4.84e-125 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| JAKIAOLI_2 | 271296 | 270628 | CiaR | 8.94e-125 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| JAKIAOLI_5 | 139561 | 140247 | CovR | 4.23e-120 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| JAKIAOLI_2 | 2422 | 3126 | walR | 5.11e-120 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| JAKIAOLI_8 | 758 | 363 | SpxA2 | 4.28e-76 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| JAKIAOLI_2 | 130918 | 130517 | SpxA1 | 1.03e-69 | transcriptional regulator | 82.84 | 100.00 | ERL19273 |
| JAKIAOLI_7 | 6436 | 6032 | GrpE | 9.89e-68 | protein grpE | 82.96 | 78.82 | ABP89267 |
| JAKIAOLI_5 | 162771 | 163058 | spr0479 | 1.66e-47 | essential gene | 84.38 | 98.97 | AAK99283 |
| JAKIAOLI_1 | 43629 | 43883 | spr1327 | 2.57e-44 | essential gene | 92.94 | 100.00 | AAL00131 |
| JAKIAOLI_4 | 113970 | 114233 | spr0175 | 1.02e-42 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| JAKIAOLI_4 | 114794 | 115060 | spr0177 | 2.26e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| JAKIAOLI_5 | 3313 | 3804 | RopE (M5005_Spy_1611) | 9.36e-73 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| JAKIAOLI_3 | 136972 | 134672 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.09 | 98.08 | MYN69560 |
| JAKIAOLI_4 | 63727 | 62708 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.35 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| JAKIAOLI_1 | 0.0 | 1.0 | 0.0 |
| JAKIAOLI_2 | 0.0 | 1.0 | 0.0 |
| JAKIAOLI_3 | 0.0 | 1.0 | 0.0 |
| JAKIAOLI_4 | 0.0 | 1.0 | 0.0 |
| JAKIAOLI_5 | 0.0 | 1.0 | 0.0 |
| JAKIAOLI_6 | 0.0 | 1.0 | 0.0 |
| JAKIAOLI_7 | 0.0 | 1.0 | 0.0 |
| JAKIAOLI_8 | 0.0 | 1.0 | 0.0 |
| JAKIAOLI_9 | 0.0 | 1.0 | 0.0 |
| JAKIAOLI_10 | 0.0 | 1.0 | 0.0 |
| JAKIAOLI_11 | 1.0 | 0.0 | 1.0 |
| JAKIAOLI_12 | 0.0 | 1.0 | 0.0 |
| JAKIAOLI_14 | 0.0 | 1.0 | 0.0 |
| JAKIAOLI_15 | 0.0 | 1.0 | 0.0 |
| JAKIAOLI_16 | 0.0 | 1.0 | 0.0 |
| JAKIAOLI_17 | 0.0 | 1.0 | 0.0 |
| JAKIAOLI_18 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| JAKIAOLI_1 | 216842 | 262037 | False | Siphoviridae | VOG0275, VOG7508, VOG4602, VOG0743, VOG3622, VOG6425, VOG6915, VOG1564, VOG9469, VOG4908, VOG2246, VOG0651, VOG0283, VOG0685, VOG0226, VOG4693, VOG0655, VOG0656, VOG6414, VOG0152, VOG0044, VOG3468, VOG0656, VOG0152, VOG11077, VOG4567, VOG0657, VOG0658, VOG0198, VOG0796, VOG4544, VOG0720, VOG4564, VOG4555, VOG3434, VOG1887, VOG3435, VOG4713, VOG0723, VOG5660, VOG0724, VOG0799, VOG0725, VOG0800, VOG3636, VOG4605, VOG4599, VOG0647, VOG4782, VOG3298, VOG7888, VOG0054, VOG5034, VOG7557, VOG4025, VOG10230 |
Phigaro v2.4.0 (Database: Jan. 2024 release)