Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 9 | 18.03 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KEODECGH_1 | 419100 | 420746 | vmlR | ARO:3004476 | vmlR | 97.81 | 100.18 | NC_000964.3 |
| KEODECGH_6 | 258568 | 261138 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| KEODECGH_4 | 221402 | 222256 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| KEODECGH_4 | 206371 | 207573 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| KEODECGH_1 | 152437 | 153030 | tmrB | ARO:3003059 | tmrB | 96.95 | 100.00 | AL009126.3 |
| KEODECGH_7 | 109548 | 109865 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| KEODECGH_7 | 109210 | 109548 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| KEODECGH_1 | 102092 | 103531 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.42 | JYFL01000006.1 |
| KEODECGH_1 | 89280 | 90200 | mphK | ARO:3004541 | mphK | 94.77 | 100.00 | CP010314.1 |
| KEODECGH_4 | 17026 | 18195 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KEODECGH_4 | 221402 | 222256 | aadK | Streptomycin | 98.95 | 100.00 | M26879 |
| KEODECGH_1 | 89280 | 90200 | mph(K) | Spiramycin, Telithromycin | 95.55 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KEODECGH_4 | 221405 | 222256 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| KEODECGH_1 | 419103 | 420746 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 97.81 | 100.00 | WP_003234144.1 |
| KEODECGH_1 | 89280 | 90197 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 94.77 | 100.00 | WP_003246254.1 |
| KEODECGH_9 | 160786 | 161304 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.33 | 100.00 | WP_003242546.1 |
| KEODECGH_5 | 154806 | 157403 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.17 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KEODECGH_11 | 3335 | 4516 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| KEODECGH_2 | 326203 | 327141 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.57 | 100.00 | NP_391077 |
| KEODECGH_5 | 255678 | 256318 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 99.84 | NP_390062 |
| KEODECGH_2 | 327161 | 334297 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.50 | 100.00 | NP_391076 |
| KEODECGH_2 | 322531 | 323316 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| KEODECGH_11 | 2871 | 3320 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| KEODECGH_11 | 1710 | 2852 | capA | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_391469 |
| KEODECGH_5 | 104527 | 106290 | dep/capD | 0.0 | Capsule | Immune modulation | 99.09 | 100.00 | NP_389723 |
| KEODECGH_2 | 324556 | 326175 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.15 | 100.00 | NP_391078 |
| KEODECGH_2 | 323342 | 324538 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.08 | 100.00 | NP_391079 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KEODECGH_20 | 31797 | 32933 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| KEODECGH_28 | 11369 | 10500 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| KEODECGH_8 | 150523 | 151299 | CodY | 2.09e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| KEODECGH_2 | 36780 | 36211 | ClpP | 2.32e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| KEODECGH_16 | 12020 | 11628 | spxA1 | 5.04e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| KEODECGH_16 | 12020 | 11628 | spx | 3.01e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| KEODECGH_21 | 34757 | 34515 | SpoVG | 7.52e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| KEODECGH_1 | 376338 | 376535 | CspA | 1.83e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| KEODECGH_5 | 268281 | 268475 | CspD | 1.96e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| KEODECGH_17 | 43825 | 44019 | CspB | 6.99e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| KEODECGH_32 | 1278 | 1471 | Col(SD853) | 194 / 194 | 97.94 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KEODECGH_1 | 0.0 | 1.0 | 0.0 |
| KEODECGH_2 | 0.0 | 1.0 | 0.0 |
| KEODECGH_3 | 0.0 | 1.0 | 0.0 |
| KEODECGH_4 | 0.0 | 1.0 | 0.0 |
| KEODECGH_5 | 0.0 | 1.0 | 0.0 |
| KEODECGH_6 | 0.0 | 1.0 | 0.0 |
| KEODECGH_7 | 0.0 | 1.0 | 0.0 |
| KEODECGH_8 | 0.0 | 1.0 | 0.0 |
| KEODECGH_9 | 0.0 | 1.0 | 0.0 |
| KEODECGH_10 | 0.0 | 1.0 | 0.0 |
| KEODECGH_11 | 0.0 | 1.0 | 0.0 |
| KEODECGH_12 | 0.0 | 1.0 | 0.0 |
| KEODECGH_13 | 0.0 | 1.0 | 0.0 |
| KEODECGH_14 | 0.0 | 1.0 | 0.0 |
| KEODECGH_15 | 0.0 | 1.0 | 0.0 |
| KEODECGH_16 | 0.0 | 1.0 | 0.0 |
| KEODECGH_17 | 0.0 | 1.0 | 0.0 |
| KEODECGH_18 | 0.0 | 1.0 | 0.0 |
| KEODECGH_19 | 0.0 | 1.0 | 0.0 |
| KEODECGH_20 | 0.0 | 1.0 | 0.0 |
| KEODECGH_21 | 0.0 | 1.0 | 0.0 |
| KEODECGH_22 | 0.0 | 1.0 | 0.0 |
| KEODECGH_23 | 0.0 | 1.0 | 0.0 |
| KEODECGH_24 | 0.0 | 1.0 | 0.0 |
| KEODECGH_25 | 0.0 | 1.0 | 0.0 |
| KEODECGH_26 | 0.0 | 1.0 | 0.0 |
| KEODECGH_27 | 0.0 | 1.0 | 0.0 |
| KEODECGH_28 | 0.0 | 1.0 | 0.0 |
| KEODECGH_29 | 1.0 | 0.0 | 1.0 |
| KEODECGH_30 | 0.0 | 1.0 | 0.0 |
| KEODECGH_31 | 0.0 | 1.0 | 0.0 |
| KEODECGH_32 | 1.0 | 0.0 | 1.0 |
| KEODECGH_34 | 0.0 | 1.0 | 0.0 |
| KEODECGH_35 | 1.0 | 0.0 | 1.0 |
| KEODECGH_37 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KEODECGH_2 | 121398 | 158758 | False | Siphoviridae | VOG3490, VOG0275, VOG1563, VOG9667, VOG6495, VOG7581, VOG4566, VOG0189, VOG6931, VOG6587, VOG4939, VOG0198, VOG0275, VOG5221, VOG4556, VOG4841, VOG0713, VOG4581, VOG1886, VOG3607, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG0801, VOG4772, VOG4660, VOG4612, VOG3390, VOG4705 |
| KEODECGH_7 | 54982 | 79967 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705, VOG0753 |
| KEODECGH_10 | 11185 | 37027 | False | Siphoviridae | VOG4660, VOG9712, VOG5042, VOG3389, VOG4910, VOG9312, VOG0275, VOG7930, VOG8610, VOG5008, VOG4705, VOG3390, VOG7718, VOG5534, VOG6940, VOG9713, VOG9219 |
| KEODECGH_10 | 86242 | 97167 | False | Siphoviridae | VOG0322, VOG7476, VOG0025, VOG4551, VOG0918, VOG1198, VOG4724, VOG1652 |
| KEODECGH_14 | 9047 | 32543 | False | Myoviridae | VOG2703, VOG1993, VOG4574, VOG0641, VOG4705, VOG3390, VOG7718, VOG4845, VOG4691, VOG4550, VOG0573, VOG1348, VOG1433, VOG1956, VOG6163, VOG4699, VOG1352, VOG1883, VOG1881, VOG1353, VOG4623, VOG4564, VOG4553, VOG4555, VOG0720 |
Phigaro v2.4.0 (Database: Jan. 2024 release)