Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 4 | 16.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BKFGMLEK_1 | 2755851 | 2756705 | aadK | ARO:3002627 | aadK | 97.18 | 100.00 | AL009126.1 |
| BKFGMLEK_1 | 2736513 | 2737715 | blt | ARO:3003006 | blt | 98.00 | 100.00 | L32599.1 |
| BKFGMLEK_1 | 2513845 | 2515014 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| BKFGMLEK_1 | 1371779 | 1372096 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| BKFGMLEK_1 | 1371441 | 1371779 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| BKFGMLEK_1 | 900976 | 903546 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| BKFGMLEK_1 | 596398 | 598026 | vmlR | ARO:3004476 | vmlR | 95.20 | 99.09 | NC_000964.3 |
| BKFGMLEK_1 | 327997 | 328590 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| BKFGMLEK_1 | 277439 | 278872 | lmrB | ARO:3002813 | lmrB | 100.00 | 100.00 | JYFL01000006.1 |
| BKFGMLEK_1 | 264653 | 265573 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BKFGMLEK_1 | 2755851 | 2756705 | aadK | Streptomycin | 98.13 | 100.00 | M26879 |
| BKFGMLEK_1 | 264653 | 265573 | mph(K) | Spiramycin, Telithromycin | 97.07 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BKFGMLEK_1 | 264653 | 265570 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| BKFGMLEK_1 | 2755854 | 2756705 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.18 | 100.00 | WP_003229862.1 |
| BKFGMLEK_1 | 596404 | 598026 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 95.38 | 98.90 | WP_003234144.1 |
| BKFGMLEK_1 | 4215030 | 4215542 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.23 | 98.84 | WP_003242546.1 |
| BKFGMLEK_1 | 2048465 | 2051062 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.94 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BKFGMLEK_1 | 3733106 | 3734287 | capB | 0.0 | Capsule | Immune modulation | 99.41 | 100.00 | NP_391471 |
| BKFGMLEK_1 | 3732642 | 3733091 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| BKFGMLEK_1 | 3731481 | 3732623 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| BKFGMLEK_1 | 3309544 | 3310329 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.98 | 100.00 | NP_391080 |
| BKFGMLEK_1 | 2272645 | 2273286 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.75 | 100.00 | NP_390062 |
| BKFGMLEK_1 | 3305708 | 3306646 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.72 | 100.00 | NP_391077 |
| BKFGMLEK_1 | 3308322 | 3309518 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.41 | 100.00 | NP_391079 |
| BKFGMLEK_1 | 3298558 | 3305688 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.32 | 99.99 | NP_391076 |
| BKFGMLEK_1 | 2003961 | 2005724 | dep/capD | 0.0 | Capsule | Immune modulation | 98.07 | 100.00 | NP_389723 |
| BKFGMLEK_1 | 3306674 | 3308293 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.59 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BKFGMLEK_1 | 132324 | 133460 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BKFGMLEK_1 | 18601 | 19470 | pdxS | 3.15e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BKFGMLEK_1 | 1682637 | 1683413 | CodY | 3.45e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BKFGMLEK_1 | 3575466 | 3576035 | ClpP | 2.55e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BKFGMLEK_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BKFGMLEK_1 | 1314301 | 1340415 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| BKFGMLEK_1 | 2162350 | 2169238 | False | Siphoviridae | VOG0918, VOG4551, VOG0025, VOG7476, VOG0322 |
| BKFGMLEK_1 | 2315293 | 2322854 | False | Siphoviridae | VOG4553, VOG4555, VOG0720, VOG5023, VOG0221, VOG3699, VOG8610, VOG4705 |
| BKFGMLEK_1 | 2682906 | 2715964 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG10989, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG5668, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG9315, VOG0796, VOG4585, VOG0198, VOG4058, VOG4693, VOG0189, VOG11060 |
Phigaro v2.4.0 (Database: Jan. 2024 release)