Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 1 | 2 | 7 | 7.35 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EHHMHPKN_44 | 58352 | 59401 | clbA | ARO:3002814 | clbA | 99.71 | 100.00 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| EHHMHPKN_44 | 58366 | 59401 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 89.09 | 98.67 | KR610408 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EHHMHPKN_44 | 58355 | 59401 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 99.71 | 100.00 | WP_012116915.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EHHMHPKN_33 | 28634 | 29815 | capB | 0.0 | Capsule | Immune modulation | 81.79 | 99.66 | NP_391471 |
| EHHMHPKN_33 | 28170 | 28619 | capC | 9.05e-118 | Capsule | Immune modulation | 84.03 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EHHMHPKN_64 | 31771 | 32907 | tufA | 0.0 | elongation factor Tu | 86.81 | 95.69 | AHM69299 |
| EHHMHPKN_5 | 4394 | 5263 | pdxS | 1.37e-152 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| EHHMHPKN_11 | 1365724 | 1364948 | CodY | 1.79e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| EHHMHPKN_1 | 23216 | 23608 | spxA1 | 1.28e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| EHHMHPKN_44 | 70310 | 70113 | CspA | 7.63e-31 | cold shock protein | 89.39 | 100.00 | CAA62903 |
| EHHMHPKN_61 | 41232 | 41038 | CspB | 4.85e-27 | cold shock protein | 84.61 | 98.48 | CAD00094 |
| EHHMHPKN_22 | 131247 | 130375 | lipA (SAUSA300_0829) | 1.78e-162 | lipoic acid synthetase | 80.82 | 95.41 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EHHMHPKN_1 | 0.0 | 1.0 | 0.0 |
| EHHMHPKN_2 | 0.0 | 1.0 | 0.0 |
| EHHMHPKN_3 | 0.0 | 1.0 | 0.0 |
| EHHMHPKN_4 | 0.0 | 1.0 | 0.0 |
| EHHMHPKN_5 | 0.0 | 1.0 | 0.0 |
| EHHMHPKN_6 | 0.0 | 1.0 | 0.0 |
| EHHMHPKN_11 | 0.0 | 1.0 | 0.0 |
| EHHMHPKN_22 | 0.0 | 1.0 | 0.0 |
| EHHMHPKN_33 | 0.0 | 1.0 | 0.0 |
| EHHMHPKN_44 | 0.0 | 1.0 | 0.0 |
| EHHMHPKN_55 | 0.0 | 1.0 | 0.0 |
| EHHMHPKN_61 | 0.0 | 1.0 | 0.0 |
| EHHMHPKN_62 | 0.0 | 1.0 | 0.0 |
| EHHMHPKN_63 | 0.0 | 1.0 | 0.0 |
| EHHMHPKN_64 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| EHHMHPKN_11 | 190810 | 192790 | False | Unknown | VOG1309 |
| EHHMHPKN_11 | 200337 | 226980 | False | Myoviridae | VOG6517, VOG0052, VOG0796, VOG10439, VOG0720, VOG1183, VOG0045, VOG4555, VOG4572, VOG1643, VOG4564, VOG4623, VOG1353, VOG1881, VOG1883, VOG1352, VOG4699, VOG6163, VOG1956, VOG1433, VOG1348, VOG0573, VOG4550, VOG4691, VOG4845, VOG7718, VOG3390, VOG4705, VOG0753, VOG0136, VOG4574 |
| EHHMHPKN_11 | 808241 | 834585 | False | Siphoviridae | VOG4581, VOG1886, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG7896, VOG0641, VOG10230 |
| EHHMHPKN_22 | 273286 | 297937 | False | Siphoviridae | VOG10230, VOG0641, VOG4705, VOG3390, VOG4612, VOG4660, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4544, VOG4581, VOG4841, VOG0531, VOG0275, VOG0198, VOG6242 |
| EHHMHPKN_63 | 61632 | 88014 | False | Myoviridae | VOG0136, VOG0753, VOG4705, VOG3390, VOG4612, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189 |
Phigaro v2.4.0 (Database: Jan. 2024 release)