Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 1 | 7 | 7.35 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MKMBIGCO_2 | 72066 | 73115 | clbA | ARO:3002814 | clbA | 97.42 | 100.00 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MKMBIGCO_2 | 72066 | 73101 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 87.74 | 98.67 | KR610408 |
| MKMBIGCO_38 | 132768 | 134144 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 86.56 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MKMBIGCO_2 | 72066 | 73109 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 97.70 | 99.71 | WP_012116915.1 |
| MKMBIGCO_38 | 132768 | 134138 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 86.43 | 99.78 | WP_003242953.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MKMBIGCO_30 | 378448 | 379629 | capB | 0.0 | Capsule | Immune modulation | 82.46 | 99.66 | NP_391471 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MKMBIGCO_9 | 31678 | 32814 | tufA | 0.0 | elongation factor Tu | 86.54 | 95.69 | AHM69299 |
| MKMBIGCO_12 | 4307 | 5176 | pdxS | 8.75e-153 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| MKMBIGCO_14 | 384034 | 384810 | CodY | 8.52e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MKMBIGCO_36 | 250212 | 250604 | spxA1 | 7.69e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| MKMBIGCO_2 | 42757 | 42954 | CspA | 7.13e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| MKMBIGCO_34 | 236749 | 236555 | CspD | 9.91e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| MKMBIGCO_34 | 236749 | 236552 | CspB | 2.06e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
| MKMBIGCO_2 | 42757 | 42951 | CspR | 9.34e-27 | cold shock protein | 80.00 | 98.48 | AAO82613 |
| MKMBIGCO_43 | 22162 | 23034 | lipA (SAUSA300_0829) | 4.92e-163 | lipoic acid synthetase | 80.82 | 95.41 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MKMBIGCO_1 | 0.0 | 1.0 | 0.0 |
| MKMBIGCO_2 | 0.0 | 1.0 | 0.0 |
| MKMBIGCO_4 | 0.0 | 1.0 | 0.0 |
| MKMBIGCO_5 | 0.0 | 1.0 | 0.0 |
| MKMBIGCO_6 | 0.0 | 1.0 | 0.0 |
| MKMBIGCO_7 | 0.0 | 1.0 | 0.0 |
| MKMBIGCO_9 | 0.0 | 1.0 | 0.0 |
| MKMBIGCO_10 | 0.0 | 1.0 | 0.0 |
| MKMBIGCO_12 | 0.0 | 1.0 | 0.0 |
| MKMBIGCO_13 | 0.0 | 1.0 | 0.0 |
| MKMBIGCO_14 | 0.0 | 1.0 | 0.0 |
| MKMBIGCO_17 | 0.0 | 1.0 | 0.0 |
| MKMBIGCO_19 | 1.0 | 0.0 | 1.0 |
| MKMBIGCO_30 | 0.0 | 1.0 | 0.0 |
| MKMBIGCO_34 | 0.0 | 1.0 | 0.0 |
| MKMBIGCO_36 | 0.0 | 1.0 | 0.0 |
| MKMBIGCO_38 | 0.0 | 1.0 | 0.0 |
| MKMBIGCO_40 | 0.0 | 1.0 | 0.0 |
| MKMBIGCO_41 | 0.0 | 1.0 | 0.0 |
| MKMBIGCO_42 | 0.0 | 1.0 | 0.0 |
| MKMBIGCO_43 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MKMBIGCO_14 | 3767 | 11427 | False | Myoviridae | VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705 |
| MKMBIGCO_34 | 179634 | 182399 | False | Unknown | VOG9667, VOG9667, VOG0650, VOG4552 |
| MKMBIGCO_34 | 191682 | 216991 | False | Siphoviridae | VOG4581, VOG1886, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG7896, VOG0641, VOG10230 |
| MKMBIGCO_36 | 342029 | 351377 | False | Siphoviridae | VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749 |
Phigaro v2.4.0 (Database: Jan. 2024 release)