Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 7 | 17.83 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| JKCJBJHN_1 | 1640137 | 1640475 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| JKCJBJHN_1 | 1639820 | 1640137 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| JKCJBJHN_1 | 652448 | 653617 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| JKCJBJHN_1 | 455536 | 456738 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| JKCJBJHN_1 | 437501 | 438355 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| JKCJBJHN_3 | 343510 | 344430 | mphK | ARO:3004541 | mphK | 98.69 | 100.00 | CP010314.1 |
| JKCJBJHN_3 | 330178 | 331617 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.42 | JYFL01000006.1 |
| JKCJBJHN_3 | 280527 | 281120 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| JKCJBJHN_4 | 258863 | 261433 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| JKCJBJHN_3 | 29075 | 30718 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| JKCJBJHN_2 | 38065 | 39441 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.91 | 100.00 | D12567 |
| JKCJBJHN_1 | 437501 | 438353 | aadK | Streptomycin | 98.71 | 99.77 | M26879 |
| JKCJBJHN_3 | 343510 | 344430 | mph(K) | Spiramycin, Telithromycin | 98.48 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| JKCJBJHN_2 | 38065 | 39438 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.91 | 100.00 | WP_063856081.1 |
| JKCJBJHN_3 | 343513 | 344430 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.69 | 100.00 | WP_003246254.1 |
| JKCJBJHN_3 | 29075 | 30715 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| JKCJBJHN_1 | 437501 | 438352 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| JKCJBJHN_2 | 41394 | 41912 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| JKCJBJHN_1 | 958810 | 961407 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.83 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| JKCJBJHN_2 | 951679 | 952464 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| JKCJBJHN_2 | 955362 | 956300 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| JKCJBJHN_2 | 536446 | 537627 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| JKCJBJHN_2 | 956320 | 963456 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.58 | 100.00 | NP_391076 |
| JKCJBJHN_1 | 1010180 | 1011943 | dep/capD | 0.0 | Capsule | Immune modulation | 99.38 | 100.00 | NP_389723 |
| JKCJBJHN_2 | 952490 | 953686 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391079 |
| JKCJBJHN_1 | 852938 | 853579 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.22 | 100.00 | NP_390062 |
| JKCJBJHN_2 | 538110 | 539252 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| JKCJBJHN_2 | 537642 | 538091 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
| JKCJBJHN_2 | 953715 | 955334 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.33 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| JKCJBJHN_5 | 31772 | 32908 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| JKCJBJHN_7 | 11269 | 10400 | pdxS | 2.58e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| JKCJBJHN_1 | 1326834 | 1326058 | CodY | 1.71e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| JKCJBJHN_2 | 696808 | 696239 | ClpP | 6.41e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| JKCJBJHN_1 | 1833557 | 1833165 | spxA1 | 1.35e-69 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| JKCJBJHN_1 | 1833557 | 1833165 | spx | 8.02e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| JKCJBJHN_6 | 20611 | 20853 | SpoVG | 7.51e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| JKCJBJHN_3 | 78658 | 78461 | CspA | 1.93e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| JKCJBJHN_1 | 0.0 | 1.0 | 0.0 |
| JKCJBJHN_2 | 0.0 | 1.0 | 0.0 |
| JKCJBJHN_3 | 0.0 | 1.0 | 0.0 |
| JKCJBJHN_4 | 0.0 | 1.0 | 0.0 |
| JKCJBJHN_5 | 0.0 | 1.0 | 0.0 |
| JKCJBJHN_6 | 0.0 | 1.0 | 0.0 |
| JKCJBJHN_7 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| JKCJBJHN_1 | 1669969 | 1697700 | False | Myoviridae | VOG0753, VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)