Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 9 | 10 | 4 | 14.04 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BOIEHILM_1 | 2765748 | 2766950 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
| BOIEHILM_1 | 1745349 | 1746518 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| BOIEHILM_1 | 1452069 | 1452386 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| BOIEHILM_1 | 968033 | 970603 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| BOIEHILM_1 | 589052 | 590698 | vmlR | ARO:3004476 | vmlR | 98.18 | 100.18 | NC_000964.3 |
| BOIEHILM_1 | 331662 | 332255 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| BOIEHILM_1 | 282457 | 283890 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BOIEHILM_1 | 263089 | 264008 | mph(K) | Spiramycin, Telithromycin | 97.72 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BOIEHILM_1 | 589055 | 590698 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.18 | 100.00 | WP_003234144.1 |
| BOIEHILM_1 | 4243540 | 4244058 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.06 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BOIEHILM_1 | 3776939 | 3778120 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| BOIEHILM_1 | 3374585 | 3375370 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| BOIEHILM_1 | 3775314 | 3776456 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| BOIEHILM_1 | 2231923 | 2233686 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| BOIEHILM_1 | 3370749 | 3371687 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.94 | 100.00 | NP_391077 |
| BOIEHILM_1 | 3373363 | 3374559 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.91 | 100.00 | NP_391079 |
| BOIEHILM_1 | 3776475 | 3776924 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| BOIEHILM_1 | 3363593 | 3370729 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.95 | 100.00 | NP_391076 |
| BOIEHILM_1 | 1997124 | 1997765 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 97.82 | 100.00 | NP_390062 |
| BOIEHILM_1 | 3371715 | 3373334 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.65 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BOIEHILM_1 | 132136 | 133272 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| BOIEHILM_1 | 18336 | 19205 | pdxS | 5.56e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| BOIEHILM_1 | 2502899 | 2502123 | CodY | 3.46e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BOIEHILM_1 | 3635680 | 3636249 | ClpP | 2.57e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| BOIEHILM_3 | 694 | 854 | Col(SD853) | 161 / 194 | 83.23 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BOIEHILM_1 | 0.0 | 1.0 | 0.0 |
| BOIEHILM_2 | 1.0 | 0.0 | 1.0 |
| BOIEHILM_3 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BOIEHILM_1 | 1395889 | 1421905 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG5384, VOG4705 |
| BOIEHILM_1 | 1661988 | 1666523 | False | Unknown | VOG0189, VOG5446, VOG0943, VOG0025, VOG4551 |
| BOIEHILM_1 | 1695070 | 1722610 | False | Siphoviridae | VOG0531, VOG4841, VOG4581, VOG4544, VOG4556, VOG4573, VOG4553, VOG5601, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4868, VOG4612, VOG3390, VOG4705, VOG0753, VOG4574, VOG0136, VOG6116, VOG10051, VOG5216 |
| BOIEHILM_1 | 2017949 | 2046453 | False | Siphoviridae | VOG8537, VOG10230, VOG3390, VOG4868, VOG9712, VOG5042, VOG3389, VOG4910, VOG9312, VOG2090, VOG2091, VOG0275, VOG7930, VOG8610, VOG4705, VOG3390, VOG7718, VOG5534, VOG6940, VOG9713, VOG9219, VOG5495, VOG6036, VOG9867, VOG7134 |
| BOIEHILM_1 | 2100190 | 2113547 | False | Siphoviridae | VOG6211, VOG0322, VOG7476, VOG0025, VOG4551, VOG0918, VOG1198, VOG5532, VOG0944, VOG1198 |
| BOIEHILM_1 | 2355126 | 2379921 | False | Siphoviridae | VOG0641, VOG4705, VOG3390, VOG4612, VOG4868, VOG4619, VOG0801, VOG6163, VOG5087, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG2935, VOG1886, VOG4581, VOG4841, VOG0198, VOG1884 |
| BOIEHILM_1 | 3215596 | 3220338 | False | Siphoviridae | VOG4555, VOG0720, VOG7734 |
| BOIEHILM_1 | 3237412 | 3261072 | False | Siphoviridae | VOG0944, VOG5532, VOG4707, VOG0641, VOG10940, VOG5535, VOG4599, VOG4605, VOG4545, VOG1323, VOG0725, VOG2621, VOG0799, VOG0724, VOG5660, VOG0816, VOG4623, VOG3480, VOG1329, VOG4555, VOG7363, VOG4564, VOG0720, VOG0796 |
| BOIEHILM_1 | 3267375 | 3273203 | False | Unknown | VOG0189, VOG1309, VOG4600, VOG1720 |
Phigaro v2.4.0 (Database: Jan. 2024 release)