Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 23 | 28 | 36.24 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| LENGFDHD_1 | 1043467 | 1044060 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| LENGFDHD_1 | 1043467 | 1044060 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| LENGFDHD_1 | 1043467 | 1044060 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| LENGFDHD_1 | 1272010 | 1272966 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.48 | 100.00 | WP_011226328 |
| LENGFDHD_1 | 1044060 | 1044929 | rfbA | 0.0 | Capsule | Immune modulation | 99.42 | 100.00 | WP_011681286 |
| LENGFDHD_1 | 20976 | 21815 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.41 | 100.00 | WP_011226733 |
| LENGFDHD_1 | 810703 | 812361 | fbp54 | 0.0 | FBPs | Adherence | 99.34 | 100.00 | WP_011227179 |
| LENGFDHD_1 | 1271217 | 1272026 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.01 | 100.00 | WP_002948020 |
| LENGFDHD_1 | 1272963 | 1274111 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | WP_041828290 |
| LENGFDHD_1 | 922112 | 922843 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.77 | 100.00 | WP_011681176 |
| LENGFDHD_1 | 1270012 | 1271217 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | WP_011226326 |
| LENGFDHD_1 | 1267144 | 1268928 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 98.60 | 100.00 | WP_011226325 |
| LENGFDHD_1 | 922112 | 922843 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.36 | 100.00 | WP_011227248 |
| LENGFDHD_1 | 922112 | 922843 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.22 | 100.00 | WP_011226048 |
| LENGFDHD_1 | 922844 | 924304 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.95 | 100.00 | WP_011681177 |
| LENGFDHD_1 | 1081533 | 1082294 | srtA | 0.0 | Sortase A | Exoenzyme | 97.64 | 100.00 | - |
| LENGFDHD_1 | 920661 | 921401 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 97.30 | 100.00 | WP_011681174 |
| LENGFDHD_1 | 1279153 | 1280004 | rfbD | 0.0 | Capsule | Immune modulation | 96.95 | 100.00 | WP_014621820 |
| LENGFDHD_1 | 922844 | 924304 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.92 | 100.21 | WP_011226049 |
| LENGFDHD_1 | 920661 | 921401 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 96.90 | 100.00 | WP_011226046 |
| LENGFDHD_1 | 920666 | 921401 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 96.60 | 98.13 | WP_011227246 |
| LENGFDHD_1 | 922844 | 924304 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.30 | 100.21 | WP_041827043 |
| LENGFDHD_1 | 919239 | 920604 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 95.46 | 99.85 | WP_011681173 |
| LENGFDHD_1 | 921411 | 922103 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 95.39 | 99.86 | WP_011226047 |
| LENGFDHD_1 | 1268925 | 1269992 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 95.17 | 101.14 | WP_002948022 |
| LENGFDHD_1 | 921411 | 922103 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 95.09 | 100.00 | WP_011227247 |
| LENGFDHD_1 | 919237 | 920604 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.52 | 100.00 | WP_011226045 |
| LENGFDHD_1 | 988488 | 989266 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 92.69 | 99.37 | - |
| LENGFDHD_1 | 921411 | 922103 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 91.05 | 100.00 | WP_011681175 |
| LENGFDHD_1 | 1276776 | 1278050 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 89.42 | 99.92 | WP_011226331 |
| LENGFDHD_1 | 576442 | 577745 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 87.41 | 99.54 | WP_000022832 |
| LENGFDHD_1 | 1580877 | 1581887 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.96 | 99.90 | WP_002986042 |
| LENGFDHD_1 | 1041991 | 1043037 | rfbB | 0.0 | Capsule | Immune modulation | 83.30 | 99.90 | WP_002263087 |
| LENGFDHD_1 | 117629 | 118899 | tig/ropA | 0.0 | Trigger factor | Stress survival | 81.11 | 98.60 | WP_000107753 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| LENGFDHD_1 | 687148 | 688221 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| LENGFDHD_1 | 1084981 | 1085964 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.21 | 100.00 | AAK75326 |
| LENGFDHD_1 | 493121 | 494089 | spr0084 | 0.0 | pathogenitcity related | 92.88 | 98.48 | AAK98888 |
| LENGFDHD_1 | 1454348 | 1456651 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.45 | 99.22 | AAK74619 |
| LENGFDHD_1 | 1157846 | 1159327 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 91.90 | 100.00 | ABJ54480 |
| LENGFDHD_1 | 707056 | 709302 | metE | 0.0 | methionine biosynthesis enzymes | 91.86 | 100.00 | AAK74738 |
| LENGFDHD_1 | 4491 | 5462 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| LENGFDHD_1 | 1321750 | 1320599 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.06 | 99.74 | AAL00050 |
| LENGFDHD_1 | 1294051 | 1292063 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| LENGFDHD_1 | 1068801 | 1067071 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| LENGFDHD_1 | 1764172 | 1762694 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.02 | 100.00 | ADE32434 |
| LENGFDHD_1 | 576442 | 577743 | Eno | 0.0 | enolase | 85.75 | 99.77 | ABP90468 |
| LENGFDHD_1 | 51784 | 52875 | recA | 0.0 | DNA recombination and repair | 85.44 | 95.10 | AAL00560 |
| LENGFDHD_1 | 1749344 | 1747452 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.10 | 98.90 | ABP91129 |
| LENGFDHD_1 | 712227 | 710518 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| LENGFDHD_1 | 1618736 | 1619746 | pdh | 0.0 | pyruvate dehydrogenase | 82.20 | 99.70 | ABP91006 |
| LENGFDHD_1 | 844867 | 843506 | mnmE | 0.0 | central tRNA-modifying GTPase | 80.92 | 99.56 | ABP90420 |
| LENGFDHD_1 | 1668301 | 1666409 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| LENGFDHD_1 | 562006 | 562896 | bglB | 1.36e-178 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| LENGFDHD_1 | 758737 | 757799 | SP_0676 | 1.14e-166 | transcription facot | 81.15 | 97.20 | AAK74821 |
| LENGFDHD_1 | 709494 | 710351 | metF | 5.39e-164 | methionine biosynthesis enzymes | 85.66 | 99.31 | AAK74739 |
| LENGFDHD_1 | 960297 | 959593 | covR | 2.27e-130 | responder protein | 85.11 | 100.00 | AAK71319 |
| LENGFDHD_1 | 1129750 | 1129079 | rr01 | 4.23e-126 | response regulator | 84.82 | 99.56 | CAB54566 |
| LENGFDHD_1 | 716181 | 716849 | CiaR | 5.77e-124 | two-component response regulator | 87.00 | 99.55 | AAK74935 |
| LENGFDHD_1 | 716181 | 716852 | ciaR | 2.55e-122 | two component system response regulator | 85.27 | 99.12 | AIK75708 |
| LENGFDHD_1 | 960297 | 959593 | walR | 9.12e-121 | DNA-binding response regulator | 81.70 | 100.00 | ANC99504 |
| LENGFDHD_1 | 277564 | 278244 | CovR | 6.65e-117 | serves to repress GAS virulence factor-encoding genes | 81.06 | 99.12 | ACE75886 |
| LENGFDHD_1 | 53058 | 53453 | SpxA2 | 1.97e-72 | transcriptional regulator | 89.39 | 100.00 | ERL21461 |
| LENGFDHD_1 | 119433 | 119933 | RopE (M5005_Spy_1611) | 3.62e-75 | probable DNA-directed RNA polymerase subunit delta | 82.04 | 87.43 | AAK34603 |
| LENGFDHD_1 | 1454372 | 1456645 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.16 | 97.06 | MYN69560 |
| LENGFDHD_1 | 1653861 | 1652842 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| LENGFDHD_1 | 1850573 | 1851446 | rep37 | 874 / 945 | 90.96 | HM050421 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| LENGFDHD_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)