Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 1 | 6 | 6.40 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HFLGALFL_9 | 354961 | 356010 | clbA | ARO:3002814 | clbA | 97.42 | 100.00 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| HFLGALFL_9 | 354961 | 355996 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 87.74 | 98.67 | KR610408 |
| HFLGALFL_8 | 366536 | 367912 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 87.07 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HFLGALFL_9 | 354961 | 356004 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 97.70 | 99.71 | WP_012116915.1 |
| HFLGALFL_8 | 366536 | 367906 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 86.87 | 99.78 | WP_003242953.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HFLGALFL_7 | 499108 | 500289 | capB | 0.0 | Capsule | Immune modulation | 82.46 | 99.66 | NP_391471 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HFLGALFL_11 | 28107 | 26971 | tufA | 0.0 | elongation factor Tu | 86.54 | 95.69 | AHM69299 |
| HFLGALFL_13 | 12451 | 11582 | pdxS | 8.77e-153 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| HFLGALFL_6 | 737063 | 737839 | CodY | 1.26e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| HFLGALFL_6 | 251462 | 251854 | spxA1 | 2.30e-69 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| HFLGALFL_9 | 325685 | 325882 | CspA | 2.35e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| HFLGALFL_9 | 325685 | 325879 | CspR | 3.08e-26 | cold shock protein | 80.00 | 98.48 | AAO82613 |
| HFLGALFL_7 | 141549 | 140677 | lipA (SAUSA300_0829) | 3.16e-162 | lipoic acid synthetase | 80.82 | 95.41 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HFLGALFL_1 | 1.0 | 0.0 | 1.0 |
| HFLGALFL_6 | 0.0 | 1.0 | 0.0 |
| HFLGALFL_7 | 0.0 | 1.0 | 0.0 |
| HFLGALFL_8 | 0.0 | 1.0 | 0.0 |
| HFLGALFL_9 | 0.0 | 1.0 | 0.0 |
| HFLGALFL_10 | 0.0 | 1.0 | 0.0 |
| HFLGALFL_11 | 0.0 | 1.0 | 0.0 |
| HFLGALFL_12 | 0.0 | 1.0 | 0.0 |
| HFLGALFL_13 | 0.0 | 1.0 | 0.0 |
| HFLGALFL_14 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| HFLGALFL_6 | 338965 | 364539 | False | Myoviridae | VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)