Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 3 | 0 | 1 | 3.16 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| NNCLEEBI_1 | 897037 | 897531 | lnuC | ARO:3002837 | lnuC | 96.95 | 100.00 | AY928180.1 |
| NNCLEEBI_1 | 713166 | 714542 | tet(L) | ARO:3000179 | tet(L) | 99.78 | 100.00 | M11036.1 |
| NNCLEEBI_4 | 65734 | 66471 | ErmB | ARO:3000375 | ErmB | 99.18 | 98.79 | AF242872.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| NNCLEEBI_4 | 65734 | 66471 | erm(B) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 100.00 | X72021 |
| NNCLEEBI_1 | 713166 | 714542 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 100.00 | 100.00 | M29725 |
| NNCLEEBI_1 | 897037 | 897531 | lnu(C) | Lincomycin | 98.79 | 100.00 | AY928180 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| NNCLEEBI_4 | 65737 | 66471 | erm(B) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) | EXACTX | 100.00 | 100.00 | WP_001038795.1 |
| NNCLEEBI_1 | 713166 | 714539 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_001574277.1 |
| NNCLEEBI_1 | 897037 | 897528 | lnu(C) | lincosamide nucleotidyltransferase Lnu(C) | BLASTX | 96.95 | 100.00 | WP_063851341.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| NNCLEEBI_1 | 636199 | 635492 | WalR | 6.02e-115 | transcriptional regulatory protein | 85.59 | 100.43 | EPH95667 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| NNCLEEBI_1 | 0.0 | 1.0 | 0.0 |
| NNCLEEBI_2 | 1.0 | 0.0 | 1.0 |
| NNCLEEBI_3 | 1.0 | 0.0 | 1.0 |
| NNCLEEBI_4 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| NNCLEEBI_2 | 18436 | 38057 | False | Siphoviridae | VOG4864, VOG0866, VOG4841, VOG4581, VOG4544, VOG4556, VOG4568, VOG4553, VOG0204, VOG0539, VOG0704, VOG4586, VOG2225, VOG6163, VOG4828 |
| NNCLEEBI_4 | 31809 | 36918 | False | Siphoviridae | VOG5532, VOG0944, VOG4564, VOG0796, VOG9503, VOG0689, VOG8681 |
Phigaro v2.4.0 (Database: Jan. 2024 release)