Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 4 | 0 | 0 | 4.00 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| NLHLMEME_3 | 207706 | 211269 | Bifidobacterium adolescentis rpoB mutants conferring resistance to rifampicin | ARO:3004480 | Bado_rpoB_RIF | 92.48 | 100.08 | NC_008618.1 |
| NLHLMEME_4 | 173619 | 174527 | aad(6) | ARO:3002628 | aad(6) | 92.75 | 109.42 | AY712687.1 |
| NLHLMEME_4 | 173080 | 173622 | SAT-4 | ARO:3002897 | SAT-4 | 99.44 | 100.00 | U01945.1 |
| NLHLMEME_4 | 172193 | 172987 | APH(3')-IIIa | ARO:3002647 | APH(3')-IIIa | 100.00 | 100.00 | CP004067.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| NLHLMEME_4 | 172193 | 172987 | aph(3')-III | Kanamycin, Amikacin, Neomycin, Butirosin, Isepamicin, Lividomycin, Paromomycin, Ribostamycin | 100.00 | 100.00 | M26832 |
| NLHLMEME_4 | 173619 | 174527 | ant(6)-Ia | Streptomycin | 92.76 | 99.78 | AF330699 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| NLHLMEME_4 | 173622 | 174527 | ant(6)-Ia | aminoglycoside nucleotidyltransferase ANT(6)-Ia | EXACTX | 100.00 | 100.00 | WP_001255864.1 |
| NLHLMEME_4 | 172196 | 172987 | aph(3')-IIIa | aminoglycoside O-phosphotransferase APH(3')-IIIa | EXACTX | 100.00 | 100.00 | WP_001096887.1 |
| NLHLMEME_4 | 173083 | 173622 | sat4 | streptothricin N-acetyltransferase Sat4 | EXACTX | 100.00 | 100.00 | WP_000627290.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| NLHLMEME_1 | 0.0 | 1.0 | 0.0 |
| NLHLMEME_2 | 0.0 | 1.0 | 0.0 |
| NLHLMEME_3 | 0.0 | 1.0 | 0.0 |
| NLHLMEME_4 | 0.0 | 1.0 | 0.0 |
| NLHLMEME_5 | 0.0 | 1.0 | 0.0 |
| NLHLMEME_6 | 0.0 | 1.0 | 0.0 |
| NLHLMEME_7 | 0.0 | 1.0 | 0.0 |
| NLHLMEME_8 | 0.0 | 1.0 | 0.0 |
| NLHLMEME_9 | 0.0 | 1.0 | 0.0 |
| NLHLMEME_10 | 0.0 | 1.0 | 0.0 |
| NLHLMEME_11 | 0.0 | 1.0 | 0.0 |
| NLHLMEME_12 | 0.0 | 1.0 | 0.0 |
| NLHLMEME_13 | 0.0 | 1.0 | 0.0 |
| NLHLMEME_14 | 0.0 | 1.0 | 0.0 |
| NLHLMEME_15 | 0.0 | 1.0 | 0.0 |
| NLHLMEME_16 | 0.0 | 1.0 | 0.0 |
| NLHLMEME_17 | 0.0 | 1.0 | 0.0 |
| NLHLMEME_19 | 0.0 | 1.0 | 0.0 |
| NLHLMEME_20 | 0.0 | 1.0 | 0.0 |
| NLHLMEME_21 | 0.0 | 1.0 | 0.0 |
| NLHLMEME_22 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| NLHLMEME_3 | 12926 | 15005 | False | Unknown | VOG3298, VOG7888 |
| NLHLMEME_5 | 152734 | 159613 | False | Siphoviridae | VOG5793, VOG5588, VOG5589, VOG5587, VOG4568 |
| NLHLMEME_8 | 52826 | 54812 | False | Unknown | VOG4600 |
| NLHLMEME_8 | 61079 | 69275 | False | Siphoviridae | VOG4544, VOG4556, VOG4568, VOG4553, VOG1210, VOG1158, VOG1159, VOG4621 |
Phigaro v2.4.0 (Database: Jan. 2024 release)