Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 3 | 15.91 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FLDNKFCL_3 | 4061044 | 4063614 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| FLDNKFCL_3 | 3769671 | 3771314 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
| FLDNKFCL_3 | 3498534 | 3499127 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| FLDNKFCL_3 | 3448013 | 3449446 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| FLDNKFCL_3 | 3435200 | 3436120 | mphK | ARO:3004541 | mphK | 99.02 | 100.00 | CP010314.1 |
| FLDNKFCL_3 | 1659115 | 1659969 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| FLDNKFCL_3 | 1640361 | 1641563 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
| FLDNKFCL_3 | 1419068 | 1420237 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| FLDNKFCL_3 | 435977 | 436294 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| FLDNKFCL_3 | 435639 | 435977 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FLDNKFCL_3 | 3435200 | 3436120 | mph(K) | Spiramycin, Telithromycin | 99.35 | 100.00 | NC_000964 |
| FLDNKFCL_3 | 1659115 | 1659969 | aadK | Streptomycin | 98.71 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FLDNKFCL_3 | 3435200 | 3436117 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.02 | 100.00 | WP_003246254.1 |
| FLDNKFCL_3 | 1659118 | 1659969 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| FLDNKFCL_3 | 3769674 | 3771314 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| FLDNKFCL_3 | 3138645 | 3139163 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.64 | 100.00 | WP_003242546.1 |
| FLDNKFCL_3 | 1123560 | 1126157 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.65 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FLDNKFCL_3 | 2645557 | 2646738 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| FLDNKFCL_3 | 2222711 | 2223496 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| FLDNKFCL_3 | 1221759 | 1222400 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| FLDNKFCL_3 | 2221489 | 2222685 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| FLDNKFCL_3 | 1069316 | 1071079 | dep/capD | 0.0 | Capsule | Immune modulation | 99.32 | 100.00 | NP_389723 |
| FLDNKFCL_3 | 2645093 | 2645542 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| FLDNKFCL_3 | 2643932 | 2645074 | capA | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | NP_391469 |
| FLDNKFCL_3 | 2211719 | 2218855 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.72 | 100.00 | NP_391076 |
| FLDNKFCL_3 | 2218875 | 2219813 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.40 | 100.00 | NP_391077 |
| FLDNKFCL_3 | 2219841 | 2221460 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.41 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FLDNKFCL_3 | 3300227 | 3301363 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| FLDNKFCL_3 | 3186500 | 3187369 | pdxS | 3.04e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| FLDNKFCL_3 | 2486298 | 2486867 | ClpP | 2.46e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FLDNKFCL_2 | 1.0 | 0.0 | 1.0 |
| FLDNKFCL_3 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FLDNKFCL_2 | 19292 | 48219 | False | Siphoviridae | VOG4615, VOG0703, VOG3616, VOG1024, VOG10944, VOG9244, VOG4599, VOG4605, VOG6163, VOG5852, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4544, VOG4581, VOG0221, VOG10500, VOG9096 |
| FLDNKFCL_2 | 66212 | 75433 | False | Siphoviridae | VOG0025, VOG4551, VOG0918, VOG1574, VOG5292, VOG4549, VOG2725, VOG3851 |
| FLDNKFCL_3 | 379784 | 405774 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| FLDNKFCL_3 | 1590762 | 1620798 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600 |
Phigaro v2.4.0 (Database: Jan. 2024 release)