Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 8 | 17.55 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OBHDJAFP_10 | 639414 | 640583 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| OBHDJAFP_9 | 508571 | 508909 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| OBHDJAFP_9 | 508254 | 508571 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| OBHDJAFP_10 | 458576 | 459778 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| OBHDJAFP_10 | 441829 | 442683 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| OBHDJAFP_13 | 340887 | 341807 | mphK | ARO:3004541 | mphK | 98.37 | 100.00 | CP010314.1 |
| OBHDJAFP_13 | 327553 | 328992 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.42 | JYFL01000006.1 |
| OBHDJAFP_13 | 277843 | 278436 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| OBHDJAFP_13 | 29082 | 30728 | vmlR | ARO:3004476 | vmlR | 99.09 | 100.18 | NC_000964.3 |
| OBHDJAFP_12 | 27301 | 29871 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| OBHDJAFP_13 | 340887 | 341807 | mph(K) | Spiramycin, Telithromycin | 99.02 | 100.00 | NC_000964 |
| OBHDJAFP_10 | 441829 | 442683 | aadK | Streptomycin | 98.48 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OBHDJAFP_13 | 29082 | 30725 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.09 | 100.00 | WP_003234144.1 |
| OBHDJAFP_13 | 340890 | 341807 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.37 | 100.00 | WP_003246254.1 |
| OBHDJAFP_10 | 441829 | 442680 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| OBHDJAFP_3 | 38171 | 38689 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 92.49 | 100.00 | WP_003242546.1 |
| OBHDJAFP_10 | 935411 | 938008 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.65 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| OBHDJAFP_3 | 951650 | 952435 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| OBHDJAFP_3 | 520326 | 521507 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| OBHDJAFP_10 | 838221 | 838862 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| OBHDJAFP_3 | 952461 | 953657 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| OBHDJAFP_10 | 990560 | 992323 | dep/capD | 0.0 | Capsule | Immune modulation | 99.26 | 100.00 | NP_389723 |
| OBHDJAFP_3 | 521522 | 521971 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| OBHDJAFP_3 | 955333 | 956271 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.83 | 100.00 | NP_391077 |
| OBHDJAFP_3 | 956291 | 963427 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.72 | 100.00 | NP_391076 |
| OBHDJAFP_3 | 521990 | 523132 | capA | 0.0 | Capsule | Immune modulation | 98.60 | 100.00 | NP_391469 |
| OBHDJAFP_3 | 953686 | 955305 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.04 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| OBHDJAFP_1 | 28052 | 26916 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| OBHDJAFP_4 | 11246 | 10377 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| OBHDJAFP_9 | 193664 | 192888 | CodY | 5.05e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| OBHDJAFP_3 | 679555 | 678986 | ClpP | 6.41e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| OBHDJAFP_11 | 35505 | 35113 | spxA1 | 1.99e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| OBHDJAFP_11 | 35505 | 35113 | spx | 1.19e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| OBHDJAFP_2 | 34724 | 34482 | SpoVG | 7.50e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| OBHDJAFP_13 | 77124 | 76927 | CspA | 1.87e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| OBHDJAFP_11 | 281253 | 281447 | CspB | 2.87e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| OBHDJAFP_1 | 0.0 | 1.0 | 0.0 |
| OBHDJAFP_2 | 0.0 | 1.0 | 0.0 |
| OBHDJAFP_3 | 0.0 | 1.0 | 0.0 |
| OBHDJAFP_4 | 0.0 | 1.0 | 0.0 |
| OBHDJAFP_5 | 0.0 | 1.0 | 0.0 |
| OBHDJAFP_8 | 0.0 | 1.0 | 0.0 |
| OBHDJAFP_9 | 0.0 | 1.0 | 0.0 |
| OBHDJAFP_10 | 0.0 | 1.0 | 0.0 |
| OBHDJAFP_11 | 0.0 | 1.0 | 0.0 |
| OBHDJAFP_12 | 0.0 | 1.0 | 0.0 |
| OBHDJAFP_13 | 0.0 | 1.0 | 0.0 |
| OBHDJAFP_14 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| OBHDJAFP_9 | 539891 | 565948 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)