Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 8 | 18.25 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| CIDNJAAC_9 | 427543 | 427860 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| CIDNJAAC_9 | 427205 | 427543 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| CIDNJAAC_10 | 385042 | 386211 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| CIDNJAAC_13 | 323731 | 324651 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
| CIDNJAAC_13 | 310398 | 311837 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.42 | JYFL01000006.1 |
| CIDNJAAC_4 | 293543 | 296113 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| CIDNJAAC_13 | 260764 | 261357 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| CIDNJAAC_10 | 157855 | 159057 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| CIDNJAAC_10 | 139542 | 140396 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| CIDNJAAC_13 | 29353 | 30996 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.00 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| CIDNJAAC_5 | 889010 | 890386 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 98.84 | 100.00 | X08034 |
| CIDNJAAC_10 | 139542 | 140394 | aadK | Streptomycin | 98.71 | 99.77 | M26879 |
| CIDNJAAC_13 | 323731 | 324651 | mph(K) | Spiramycin, Telithromycin | 97.72 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| CIDNJAAC_5 | 889013 | 890386 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_003242953.1 |
| CIDNJAAC_13 | 29353 | 30993 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| CIDNJAAC_10 | 139542 | 140393 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| CIDNJAAC_13 | 323734 | 324651 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| CIDNJAAC_5 | 886536 | 887054 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| CIDNJAAC_8 | 184316 | 186913 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| CIDNJAAC_5 | 7487 | 8425 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| CIDNJAAC_5 | 404241 | 405422 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| CIDNJAAC_8 | 280463 | 281104 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| CIDNJAAC_5 | 8453 | 10072 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.26 | 100.00 | NP_391078 |
| CIDNJAAC_5 | 11323 | 12108 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.24 | 100.00 | NP_391080 |
| CIDNJAAC_5 | 402616 | 403758 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| CIDNJAAC_5 | 10101 | 11297 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.91 | 100.00 | NP_391079 |
| CIDNJAAC_8 | 137375 | 139138 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | NP_389723 |
| CIDNJAAC_5 | 331 | 7467 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.82 | 100.00 | NP_391076 |
| CIDNJAAC_5 | 403777 | 404226 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| CIDNJAAC_17 | 31943 | 33079 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| CIDNJAAC_20 | 11466 | 10597 | pdxS | 1.15e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| CIDNJAAC_9 | 737489 | 738265 | CodY | 7.48e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| CIDNJAAC_5 | 262842 | 263411 | ClpP | 5.53e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| CIDNJAAC_9 | 275761 | 276153 | spxA1 | 5.88e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| CIDNJAAC_9 | 275761 | 276153 | spx | 3.51e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| CIDNJAAC_16 | 34890 | 34648 | SpoVG | 7.56e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| CIDNJAAC_13 | 77724 | 77527 | CspA | 1.83e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| CIDNJAAC_8 | 293029 | 293223 | CspD | 2.11e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| CIDNJAAC_2 | 0.0 | 1.0 | 0.0 |
| CIDNJAAC_3 | 0.0 | 1.0 | 0.0 |
| CIDNJAAC_4 | 0.0 | 1.0 | 0.0 |
| CIDNJAAC_5 | 0.0 | 1.0 | 0.0 |
| CIDNJAAC_6 | 1.0 | 0.0 | 1.0 |
| CIDNJAAC_7 | 0.0 | 1.0 | 0.0 |
| CIDNJAAC_8 | 0.0 | 1.0 | 0.0 |
| CIDNJAAC_9 | 0.0 | 1.0 | 0.0 |
| CIDNJAAC_10 | 0.0 | 1.0 | 0.0 |
| CIDNJAAC_11 | 0.0 | 1.0 | 0.0 |
| CIDNJAAC_12 | 0.0 | 1.0 | 0.0 |
| CIDNJAAC_13 | 0.0 | 1.0 | 0.0 |
| CIDNJAAC_14 | 1.0 | 0.0 | 1.0 |
| CIDNJAAC_15 | 0.0 | 1.0 | 0.0 |
| CIDNJAAC_16 | 0.0 | 1.0 | 0.0 |
| CIDNJAAC_17 | 0.0 | 1.0 | 0.0 |
| CIDNJAAC_18 | 0.0 | 1.0 | 0.0 |
| CIDNJAAC_19 | 0.0 | 1.0 | 0.0 |
| CIDNJAAC_20 | 0.0 | 1.0 | 0.0 |
| CIDNJAAC_21 | 0.0 | 1.0 | 0.0 |
| CIDNJAAC_22 | 1.0 | 0.0 | 1.0 |
| CIDNJAAC_23 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| CIDNJAAC_4 | 10301 | 16958 | False | Siphoviridae | VOG9667, VOG11075, VOG0650, VOG4552, VOG4603, VOG8233, VOG0186, VOG4566 |
| CIDNJAAC_4 | 23251 | 47394 | False | Myoviridae | VOG0796, VOG0720, VOG4555, VOG4553, VOG0815, VOG4564, VOG4623, VOG1353, VOG1881, VOG1883, VOG1352, VOG4699, VOG1350, VOG6163, VOG1956, VOG1433, VOG1348, VOG0573, VOG4550, VOG4691, VOG4845, VOG7718, VOG3390, VOG4705, VOG5008, VOG0753, VOG0136, VOG4574 |
| CIDNJAAC_6 | 820 | 15763 | False | Siphoviridae | VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG5852, VOG6163, VOG4605, VOG4599, VOG9244, VOG10944, VOG1024 |
| CIDNJAAC_6 | 32821 | 47300 | False | Unknown | VOG1013, VOG0052, VOG11080, VOG2352, VOG0397, VOG1198, VOG4561, VOG4714, VOG2352, VOG3851, VOG2725 |
| CIDNJAAC_9 | 370276 | 397728 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG4612, VOG4705 |
| CIDNJAAC_10 | 175905 | 208034 | False | Myoviridae | VOG4600, VOG1309, VOG11060, VOG0189, VOG4693, VOG4567, VOG4673, VOG7060, VOG0796, VOG9315, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| CIDNJAAC_19 | 21761 | 39098 | False | Unknown | VOG9712, VOG5042, VOG3389, VOG4910, VOG9312, VOG0275, VOG7930, VOG8610, VOG5008, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)