Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 7 | 16.43 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HBDJLCNB_5 | 612232 | 613434 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| HBDJLCNB_5 | 593870 | 594724 | aadK | ARO:3002627 | aadK | 100.00 | 100.00 | AL009126.1 |
| HBDJLCNB_4 | 427799 | 428116 | ykkD | ARO:3003064 | ykkD | 100.00 | 100.00 | AL009126.1 |
| HBDJLCNB_4 | 427461 | 427799 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| HBDJLCNB_11 | 358250 | 359170 | mphK | ARO:3004541 | mphK | 100.00 | 100.00 | CP010314.1 |
| HBDJLCNB_11 | 344917 | 346356 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.42 | JYFL01000006.1 |
| HBDJLCNB_1 | 276759 | 279329 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
| HBDJLCNB_11 | 28607 | 30250 | vmlR | ARO:3004476 | vmlR | 100.00 | 100.00 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| HBDJLCNB_5 | 593870 | 594724 | aadK | Streptomycin | 100.00 | 100.00 | M26879 |
| HBDJLCNB_11 | 358250 | 359170 | mph(K) | Spiramycin, Telithromycin | 100.00 | 100.00 | NC_000964 |
| HBDJLCNB_16 | 39666 | 41043 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.93 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HBDJLCNB_5 | 593870 | 594721 | aadK | aminoglycoside 6-adenylyltransferase AadK | EXACTX | 100.00 | 100.00 | WP_003229862.1 |
| HBDJLCNB_11 | 358253 | 359170 | mphK | macrolide 2'-phosphotransferase MphK | EXACTX | 100.00 | 100.00 | WP_003246254.1 |
| HBDJLCNB_16 | 43043 | 43561 | satA | streptothricin N-acetyltransferase SatA | EXACTX | 100.00 | 100.00 | WP_003242546.1 |
| HBDJLCNB_11 | 28607 | 30247 | vmlR | ABC-F type ribosomal protection protein VmlR | EXACTX | 100.00 | 100.00 | WP_003234144.1 |
| HBDJLCNB_5 | 1276516 | 1279110 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.15 | 99.88 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HBDJLCNB_16 | 529865 | 531007 | capA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391469 |
| HBDJLCNB_16 | 528201 | 529382 | capB | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391471 |
| HBDJLCNB_16 | 529397 | 529846 | capC | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391470 |
| HBDJLCNB_5 | 38044 | 38829 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391080 |
| HBDJLCNB_5 | 38855 | 40051 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391079 |
| HBDJLCNB_5 | 40080 | 41699 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391078 |
| HBDJLCNB_5 | 1034487 | 1035128 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
| HBDJLCNB_5 | 42686 | 49824 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.97 | 100.00 | NP_391076 |
| HBDJLCNB_5 | 1324003 | 1325765 | dep/capD | 0.0 | Capsule | Immune modulation | 99.94 | 99.94 | NP_389723 |
| HBDJLCNB_5 | 41727 | 42666 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.89 | 100.00 | NP_391077 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HBDJLCNB_15 | 34635 | 35771 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| HBDJLCNB_3 | 75437 | 74568 | pdxS | 6.10e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| HBDJLCNB_4 | 741081 | 741857 | CodY | 6.89e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| HBDJLCNB_16 | 682618 | 682049 | ClpP | 5.40e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| HBDJLCNB_4 | 278630 | 279022 | spxA1 | 2.04e-69 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| HBDJLCNB_3 | 38657 | 38415 | SpoVG | 1.12e-38 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| HBDJLCNB_11 | 75741 | 75544 | CspA | 2.00e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HBDJLCNB_1 | 0.0 | 1.0 | 0.0 |
| HBDJLCNB_3 | 0.0 | 1.0 | 0.0 |
| HBDJLCNB_4 | 0.0 | 1.0 | 0.0 |
| HBDJLCNB_5 | 0.0 | 1.0 | 0.0 |
| HBDJLCNB_6 | 0.0 | 1.0 | 0.0 |
| HBDJLCNB_7 | 1.0 | 0.0 | 1.0 |
| HBDJLCNB_9 | 0.0 | 1.0 | 0.0 |
| HBDJLCNB_10 | 0.0 | 1.0 | 0.0 |
| HBDJLCNB_11 | 0.0 | 1.0 | 0.0 |
| HBDJLCNB_12 | 0.0 | 1.0 | 0.0 |
| HBDJLCNB_14 | 0.0 | 1.0 | 0.0 |
| HBDJLCNB_15 | 0.0 | 1.0 | 0.0 |
| HBDJLCNB_16 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| HBDJLCNB_4 | 372271 | 396740 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| HBDJLCNB_5 | 632986 | 663361 | False | Myoviridae | VOG4600, VOG1309, VOG11060, VOG0189, VOG4693, VOG9587, VOG4673, VOG0796, VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4691, VOG4862, VOG4862, VOG7718 |
| HBDJLCNB_7 | 15720 | 37531 | False | Siphoviridae | VOG0221, VOG0296, VOG1886, VOG4556, VOG4568, VOG4553, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG5852, VOG6163, VOG4605, VOG4599, VOG9244, VOG10944, VOG1024 |
| HBDJLCNB_7 | 56945 | 60370 | False | Unknown | VOG0052, VOG11080, VOG2352 |
Phigaro v2.4.0 (Database: Jan. 2024 release)