Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 24 | 30 | 38.42 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MLHHEAKI_1 | 1682992 | 1683843 | rfbD | 0.0 | Capsule | Immune modulation | 95.19 | 100.00 | WP_014621820 |
| MLHHEAKI_1 | 1057102 | 1057842 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 95.01 | 100.00 | WP_011681174 |
| MLHHEAKI_1 | 1057102 | 1057842 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 94.33 | 100.00 | WP_011226046 |
| MLHHEAKI_1 | 1054199 | 1055659 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 94.11 | 100.14 | WP_011226049 |
| MLHHEAKI_1 | 1054199 | 1055659 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 94.05 | 99.93 | WP_011681177 |
| MLHHEAKI_1 | 1057899 | 1059266 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.01 | 100.00 | WP_011226045 |
| MLHHEAKI_1 | 1057102 | 1057837 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 93.75 | 98.13 | WP_011227246 |
| MLHHEAKI_1 | 1680620 | 1681891 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 93.71 | 99.76 | WP_011226331 |
| MLHHEAKI_1 | 1057899 | 1059264 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 93.70 | 99.85 | WP_011681173 |
| MLHHEAKI_1 | 1679662 | 1680617 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 93.41 | 94.56 | WP_011226330 |
| MLHHEAKI_1 | 1054199 | 1055659 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 93.22 | 100.14 | WP_041827043 |
| MLHHEAKI_1 | 1671010 | 1672794 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 92.61 | 99.94 | WP_011226325 |
| MLHHEAKI_1 | 1675844 | 1676800 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 92.48 | 100.00 | WP_011226328 |
| MLHHEAKI_1 | 1676797 | 1677945 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 92.34 | 100.00 | WP_041828290 |
| MLHHEAKI_1 | 1675051 | 1675860 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 92.23 | 99.88 | WP_002948020 |
| MLHHEAKI_1 | 1673846 | 1675051 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 92.12 | 100.00 | WP_011226326 |
| MLHHEAKI_1 | 1055660 | 1056391 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 91.53 | 100.00 | WP_011226048 |
| MLHHEAKI_1 | 1672791 | 1673825 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 91.29 | 99.15 | WP_002948022 |
| MLHHEAKI_1 | 1055660 | 1056391 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 91.12 | 100.00 | WP_011681176 |
| MLHHEAKI_1 | 882707 | 883300 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 91.08 | 100.00 | WP_011226149 |
| MLHHEAKI_1 | 38878 | 39717 | cbpD | 0.0 | Autolysin | Exoenzyme | 90.95 | 100.00 | WP_011226733 |
| MLHHEAKI_1 | 882707 | 883300 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| MLHHEAKI_1 | 882707 | 883300 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| MLHHEAKI_1 | 1163295 | 1164953 | fbp54 | 0.0 | FBPs | Adherence | 90.84 | 99.94 | WP_011227179 |
| MLHHEAKI_1 | 1055660 | 1056391 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 90.57 | 100.00 | WP_011227248 |
| MLHHEAKI_1 | 881838 | 882707 | rfbA | 0.0 | Capsule | Immune modulation | 90.46 | 100.00 | WP_011681286 |
| MLHHEAKI_1 | 1990513 | 1991523 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 88.55 | 99.80 | WP_002986042 |
| MLHHEAKI_1 | 670366 | 671669 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.24 | 99.54 | WP_000022832 |
| MLHHEAKI_1 | 1056400 | 1057092 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 88.04 | 99.86 | WP_011227247 |
| MLHHEAKI_1 | 1056400 | 1057092 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 88.04 | 99.86 | WP_011226047 |
| MLHHEAKI_1 | 1056400 | 1057092 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 87.46 | 99.86 | WP_011681175 |
| MLHHEAKI_1 | 1301845 | 1302606 | srtA | 0.0 | Sortase A | Exoenzyme | 84.40 | 99.87 | - |
| MLHHEAKI_1 | 883571 | 884617 | rfbB | 0.0 | Capsule | Immune modulation | 83.98 | 99.81 | WP_002263087 |
| MLHHEAKI_1 | 195146 | 196429 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.84 | 99.61 | WP_000107753 |
| MLHHEAKI_1 | 1068405 | 1068990 | SPD_RS01775 | 2.19e-139 | Capsule | Immune modulation | 82.28 | 96.53 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MLHHEAKI_1 | 1305296 | 1306279 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.65 | 100.00 | AAK75326 |
| MLHHEAKI_1 | 1293709 | 1292636 | MntE | 0.0 | manganese homeostasis | 95.53 | 99.72 | ABF32167 |
| MLHHEAKI_1 | 1866313 | 1868619 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.41 | 99.22 | AAK74619 |
| MLHHEAKI_1 | 1446317 | 1447798 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.32 | 100.00 | ABJ54480 |
| MLHHEAKI_1 | 581535 | 582503 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| MLHHEAKI_1 | 1274649 | 1272403 | metE | 0.0 | methionine biosynthesis enzymes | 92.52 | 100.00 | AAK74738 |
| MLHHEAKI_1 | 24455 | 25426 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| MLHHEAKI_1 | 856461 | 858191 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| MLHHEAKI_1 | 1696057 | 1694069 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.44 | 100.00 | AE014259_2 |
| MLHHEAKI_1 | 1724410 | 1723259 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| MLHHEAKI_1 | 2195070 | 2193592 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| MLHHEAKI_1 | 670366 | 671667 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| MLHHEAKI_1 | 2179737 | 2177845 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.58 | 98.90 | ABP91129 |
| MLHHEAKI_1 | 1269484 | 1271193 | PGM | 0.0 | phosphoglucomutase | 85.11 | 99.65 | ACH87041 |
| MLHHEAKI_1 | 61207 | 62298 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| MLHHEAKI_1 | 2046900 | 2047910 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| MLHHEAKI_1 | 1149532 | 1150893 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.46 | 99.56 | ABP90420 |
| MLHHEAKI_1 | 655947 | 656837 | bglB | 2.39e-176 | putative phospho--glucosidase | 87.20 | 99.66 | AAK33487 |
| MLHHEAKI_1 | 1272216 | 1271359 | metF | 5.57e-168 | methionine biosynthesis enzymes | 87.76 | 99.31 | AAK74739 |
| MLHHEAKI_1 | 1230217 | 1231167 | SP_0676 | 5.69e-168 | transcription facot | 81.07 | 98.45 | AAK74821 |
| MLHHEAKI_1 | 1180793 | 1180008 | pnuC | 1.59e-148 | NAD salvage pathway importer | 87.02 | 97.40 | AAK75931 |
| MLHHEAKI_1 | 1376738 | 1376067 | rr01 | 4.74e-126 | response regulator | 84.82 | 99.56 | CAB54566 |
| MLHHEAKI_1 | 1008538 | 1009242 | covR | 9.84e-126 | responder protein | 85.53 | 100.00 | AAK71319 |
| MLHHEAKI_1 | 1265517 | 1264846 | ciaR | 2.12e-124 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| MLHHEAKI_1 | 1265517 | 1264849 | CiaR | 3.91e-124 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| MLHHEAKI_1 | 1008538 | 1009242 | walR | 2.24e-119 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| MLHHEAKI_1 | 339626 | 340312 | CovR | 4.83e-119 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| MLHHEAKI_1 | 62472 | 62867 | SpxA2 | 2.17e-74 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| MLHHEAKI_1 | 1138067 | 1137666 | SpxA1 | 4.52e-69 | transcriptional regulator | 82.84 | 100.00 | ERL19273 |
| MLHHEAKI_1 | 181525 | 181938 | GrpE | 1.72e-66 | protein grpE | 81.88 | 80.59 | ABP89267 |
| MLHHEAKI_1 | 196596 | 197087 | RopE (M5005_Spy_1611) | 1.07e-71 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| MLHHEAKI_1 | 1866313 | 1868613 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.09 | 98.08 | MYN69560 |
| MLHHEAKI_1 | 2083334 | 2082315 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.35 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MLHHEAKI_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MLHHEAKI_1 | 674399 | 710211 | False | Siphoviridae | VOG0743, VOG3622, VOG3478, VOG0651, VOG0083, VOG0322, VOG7263, VOG4548, VOG7264, VOG4693, VOG7265, VOG0655, VOG3468, VOG0656, VOG0152, VOG0657, VOG0044, VOG0658, VOG4987, VOG0796, VOG4544, VOG0691, VOG0692, VOG4555, VOG4711, VOG5872, VOG0695, VOG0696, VOG0697, VOG0698, VOG8357, VOG0701, VOG3636, VOG4633, VOG4828, VOG0641, VOG0054, VOG5034, VOG7557 |
Phigaro v2.4.0 (Database: Jan. 2024 release)