Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 20 | 34 | 39.50 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DDCBIDHL_33 | 69054 | 70271 | mef(A) | Erythromycin, Azithromycin | 100.00 | 100.00 | U83667 |
| DDCBIDHL_33 | 67471 | 68934 | msr(D) | Erythromycin, Azithromycin, Telithromycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 100.00 | AF274302 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DDCBIDHL_33 | 69057 | 70271 | mef(A) | macrolide efflux MFS transporter Mef(A) | EXACTX | 100.00 | 100.00 | WP_000417519.1 |
| DDCBIDHL_33 | 67474 | 68934 | msr(D) | ABC-F type ribosomal protection protein Msr(D) | EXACTX | 100.00 | 100.00 | WP_000420313.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DDCBIDHL_11 | 135637 | 136488 | rfbD | 0.0 | Capsule | Immune modulation | 95.31 | 100.00 | WP_014621820 |
| DDCBIDHL_8 | 35454 | 36185 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 94.40 | 100.00 | WP_011227248 |
| DDCBIDHL_8 | 35454 | 36185 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 93.72 | 100.00 | WP_011226048 |
| DDCBIDHL_8 | 35454 | 36185 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 93.44 | 100.00 | WP_011681176 |
| DDCBIDHL_11 | 128553 | 130309 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 92.54 | 98.43 | WP_011226325 |
| DDCBIDHL_11 | 133359 | 134315 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 92.48 | 100.00 | WP_011226328 |
| DDCBIDHL_11 | 131361 | 132566 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 92.21 | 100.00 | WP_011226326 |
| DDCBIDHL_11 | 132566 | 133375 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 92.11 | 99.88 | WP_002948020 |
| DDCBIDHL_11 | 134312 | 135460 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 91.56 | 99.91 | WP_041828290 |
| DDCBIDHL_8 | 33993 | 35453 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 91.33 | 99.86 | WP_011681177 |
| DDCBIDHL_11 | 130306 | 131340 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 91.10 | 99.15 | WP_002948022 |
| DDCBIDHL_15 | 32249 | 32842 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 91.08 | 100.00 | WP_011226149 |
| DDCBIDHL_22 | 49757 | 51415 | fbp54 | 0.0 | FBPs | Adherence | 90.96 | 99.94 | WP_011227179 |
| DDCBIDHL_15 | 32249 | 32842 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| DDCBIDHL_15 | 32249 | 32842 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| DDCBIDHL_8 | 33993 | 35453 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 90.72 | 100.00 | WP_011226049 |
| DDCBIDHL_11 | 198729 | 199634 | cbpD | 0.0 | Autolysin | Exoenzyme | 90.07 | 93.50 | WP_082302295 |
| DDCBIDHL_8 | 33993 | 35453 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 90.03 | 100.00 | WP_041827043 |
| DDCBIDHL_8 | 36194 | 36886 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 89.90 | 100.00 | WP_011226047 |
| DDCBIDHL_8 | 36194 | 36886 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 89.61 | 100.00 | WP_011227247 |
| DDCBIDHL_8 | 36194 | 36886 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.47 | 100.00 | WP_011681175 |
| DDCBIDHL_33 | 92386 | 93689 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.17 | 99.54 | WP_000022832 |
| DDCBIDHL_12 | 28672 | 29685 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 85.67 | 99.90 | WP_002986042 |
| DDCBIDHL_3 | 48116 | 48877 | srtA | 0.0 | Sortase A | Exoenzyme | 85.21 | 99.74 | - |
| DDCBIDHL_15 | 30933 | 31979 | rfbB | 0.0 | Capsule | Immune modulation | 83.49 | 99.90 | WP_002263087 |
| DDCBIDHL_19 | 1783 | 3066 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.84 | 99.61 | WP_000107753 |
| DDCBIDHL_15 | 32842 | 33711 | rfbA | 0.0 | Capsule | Immune modulation | 80.28 | 99.77 | WP_002904719 |
| DDCBIDHL_8 | 36896 | 37566 | STU_RS14635 | 6.30e-150 | Capsule | Immune modulation | 81.13 | 90.28 | WP_011226046 |
| DDCBIDHL_8 | 36896 | 37566 | STER_RS05305 | 2.93e-148 | Capsule | Immune modulation | 80.98 | 90.28 | WP_011681174 |
| DDCBIDHL_8 | 36896 | 37566 | STR_RS05250 | 2.97e-138 | Capsule | Immune modulation | 80.09 | 89.20 | WP_011227246 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DDCBIDHL_3 | 45427 | 44444 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.95 | 100.00 | AAK75326 |
| DDCBIDHL_3 | 57013 | 58086 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| DDCBIDHL_49 | 83833 | 86139 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.54 | 99.22 | AAK74619 |
| DDCBIDHL_17 | 10908 | 9427 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.32 | 100.00 | ABJ54480 |
| DDCBIDHL_3 | 76075 | 78321 | metE | 0.0 | methionine biosynthesis enzymes | 93.19 | 100.00 | AAK74738 |
| DDCBIDHL_9 | 41913 | 40945 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| DDCBIDHL_14 | 36624 | 35653 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| DDCBIDHL_1 | 7164 | 691 | PrtA | 0.0 | serine protease | 91.44 | 100.61 | ABJ55268 |
| DDCBIDHL_51 | 20206 | 18476 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| DDCBIDHL_10 | 3949 | 2798 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| DDCBIDHL_11 | 149473 | 147485 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.44 | 100.00 | AE014259_2 |
| DDCBIDHL_48 | 25833 | 27311 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| DDCBIDHL_33 | 93689 | 92388 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| DDCBIDHL_48 | 41181 | 43073 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.58 | 98.90 | ABP91129 |
| DDCBIDHL_14 | 1892 | 801 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| DDCBIDHL_13 | 40607 | 42316 | PGM | 0.0 | phosphoglucomutase | 84.59 | 99.65 | ACH87041 |
| DDCBIDHL_22 | 35831 | 37192 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.89 | 99.56 | ABP90420 |
| DDCBIDHL_47 | 77179 | 76169 | pdh | 0.0 | pyruvate dehydrogenase | 82.79 | 99.70 | ABP91006 |
| DDCBIDHL_33 | 108099 | 107209 | bglB | 1.53e-177 | putative phospho--glucosidase | 87.20 | 99.66 | AAK33487 |
| DDCBIDHL_3 | 78511 | 79368 | metF | 2.91e-170 | methionine biosynthesis enzymes | 87.76 | 99.31 | AAK74739 |
| DDCBIDHL_22 | 145533 | 146483 | SP_0676 | 3.94e-170 | transcription facot | 81.39 | 98.45 | AAK74821 |
| DDCBIDHL_1 | 31315 | 30644 | rr01 | 1.59e-127 | response regulator | 84.82 | 99.56 | CAB54566 |
| DDCBIDHL_8 | 1811 | 2515 | covR | 2.19e-127 | responder protein | 85.53 | 100.00 | AAK71319 |
| DDCBIDHL_13 | 35942 | 35271 | ciaR | 1.88e-125 | two component system response regulator | 85.71 | 99.12 | AIK75708 |
| DDCBIDHL_13 | 35942 | 35274 | CiaR | 2.33e-125 | two-component response regulator | 87.00 | 99.55 | AAK74935 |
| DDCBIDHL_8 | 1811 | 2515 | walR | 4.98e-121 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| DDCBIDHL_44 | 70933 | 71619 | CovR | 1.04e-120 | serves to repress GAS virulence factor-encoding genes | 82.10 | 100.00 | ACE75886 |
| DDCBIDHL_14 | 626 | 231 | SpxA2 | 4.01e-76 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| DDCBIDHL_22 | 24374 | 23973 | SpxA1 | 9.35e-70 | transcriptional regulator | 82.09 | 100.00 | ERL19273 |
| DDCBIDHL_23 | 6581 | 6168 | GrpE | 1.45e-68 | protein grpE | 81.88 | 80.59 | ABP89267 |
| DDCBIDHL_44 | 94135 | 94422 | spr0479 | 3.30e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| DDCBIDHL_24 | 16792 | 17046 | spr1327 | 5.89e-47 | essential gene | 94.12 | 100.00 | AAL00131 |
| DDCBIDHL_48 | 81791 | 81528 | spr0175 | 4.87e-43 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| DDCBIDHL_48 | 80966 | 80700 | spr0177 | 1.08e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| DDCBIDHL_19 | 3233 | 3724 | RopE (M5005_Spy_1611) | 1.31e-73 | probable DNA-directed RNA polymerase subunit delta | 83.54 | 85.86 | AAK34603 |
| DDCBIDHL_49 | 83833 | 86133 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.36 | 98.08 | MYN69560 |
| DDCBIDHL_47 | 40769 | 41788 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 85.88 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DDCBIDHL_1 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_2 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_3 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_4 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_5 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_6 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_7 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_8 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_9 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_10 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_11 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_12 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_13 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_14 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_15 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_16 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_17 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_18 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_19 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_20 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_21 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_22 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_23 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_24 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_25 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_26 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_27 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_28 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_29 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_32 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_33 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_34 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_36 | 1.0 | 0.0 | 1.0 |
| DDCBIDHL_40 | 1.0 | 0.0 | 1.0 |
| DDCBIDHL_42 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_44 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_47 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_48 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_49 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_50 | 0.0 | 1.0 | 0.0 |
| DDCBIDHL_51 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)