safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	25	29	38.29

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
DPGBHBGB_1	1110232	1111101	rfbA	Capsule	Immune modulation	100.00	100.00	WP_011681286
DPGBHBGB_1	1109639	1110232	STER_RS06030	Capsule	Immune modulation	100.00	100.00	WP_011226149
DPGBHBGB_1	1318776	1319585	STU_RS16245	Capsule	Immune modulation	99.88	100.00	WP_002948020
DPGBHBGB_1	1109639	1110232	STR_RS05880	Capsule	Immune modulation	99.83	100.00	WP_011226149
DPGBHBGB_1	1109639	1110232	STU_RS15260	Capsule	Immune modulation	99.66	100.00	WP_011226149
DPGBHBGB_1	1319569	1320525	STU_RS16250	Capsule	Immune modulation	99.58	100.00	WP_011226328
DPGBHBGB_1	45027	45866	cbpD	Autolysin	Exoenzyme	99.52	100.00	WP_011226733
DPGBHBGB_1	1314715	1316499	STU_RS16230	Capsule	Immune modulation	99.44	100.00	WP_011226325
DPGBHBGB_1	883553	885211	fbp54	FBPs	Adherence	99.34	100.00	WP_011227179
DPGBHBGB_1	1317571	1318776	STU_RS16240	Capsule	Immune modulation	99.25	100.00	WP_011226326
DPGBHBGB_1	1316496	1317551	STU_RS16235	Capsule	Immune modulation	99.15	100.00	WP_002948022
DPGBHBGB_1	1326705	1327556	rfbD	Capsule	Immune modulation	99.06	100.00	WP_014621820
DPGBHBGB_1	988082	988813	STER_RS05315	Capsule	Immune modulation	99.04	100.00	WP_011681176
DPGBHBGB_1	988082	988813	STU_RS14645	Capsule	Immune modulation	98.77	100.00	WP_011226048
DPGBHBGB_1	1323366	1324328	STU_RS16260	Capsule	Immune modulation	98.65	95.25	WP_011226330
DPGBHBGB_1	988082	988813	STR_RS05260	Capsule	Immune modulation	98.63	100.00	WP_011227248
DPGBHBGB_1	1320522	1321670	STU_RS16255	Capsule	Immune modulation	98.43	100.00	WP_041828290
DPGBHBGB_1	1054745	1055533	cshA	Cell surface hydrophobicity proteins	Adherence	98.36	99.75	-
DPGBHBGB_1	978140	979555	STER_RS05250	Capsule	Immune modulation	97.74	100.00	WP_011681165
DPGBHBGB_1	1146425	1147186	srtA	Sortase A	Exoenzyme	97.64	100.00	-
DPGBHBGB_1	1324331	1325602	STU_RS16265	Capsule	Immune modulation	97.64	99.76	WP_011226331
DPGBHBGB_1	988814	990274	STER_RS05320	Capsule	Immune modulation	97.47	100.00	WP_011681177
DPGBHBGB_1	988814	990274	STU_RS14650	Capsule	Immune modulation	96.85	100.21	WP_011226049
DPGBHBGB_1	988814	990274	STR_RS05265	Capsule	Immune modulation	95.89	100.21	WP_041827043
DPGBHBGB_1	987381	988073	STR_RS05255	Capsule	Immune modulation	95.40	99.86	WP_011227247
DPGBHBGB_1	987381	988073	STU_RS14640	Capsule	Immune modulation	95.40	99.86	WP_011226047
DPGBHBGB_1	985191	986556	STER_RS05300	Capsule	Immune modulation	95.17	99.85	WP_011681173
DPGBHBGB_1	985189	986556	STU_RS14630	Capsule	Immune modulation	93.93	100.00	WP_011226045
DPGBHBGB_1	986613	987371	STER_RS05305	Capsule	Immune modulation	93.81	100.94	WP_011681174
DPGBHBGB_1	986613	987371	STU_RS14635	Capsule	Immune modulation	93.68	100.94	WP_011226046
DPGBHBGB_1	986618	987371	STR_RS05250	Capsule	Immune modulation	93.25	100.00	WP_011227246
DPGBHBGB_1	987381	988073	STER_RS05310	Capsule	Immune modulation	89.47	100.00	WP_011681175
DPGBHBGB_1	606443	607746	eno	Streptococcal enolase	Exoenzyme	88.10	99.46	WP_000022832
DPGBHBGB_1	1613013	1614023	plr/gapA	Streptococcal plasmin receptor/GAPDH	Adherence	87.55	99.90	WP_002986042
DPGBHBGB_1	145344	146627	tig/ropA	Trigger factor	Stress survival	80.65	99.77	WP_000107753
DPGBHBGB_1	1108164	1109210	rfbB	Capsule	Immune modulation	80.61	100.00	WP_009659423

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
DPGBHBGB_1	1149873	1150856	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
DPGBHBGB_1	714267	715340	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
DPGBHBGB_1	522760	523728	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
DPGBHBGB_1	1491210	1493513	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
DPGBHBGB_1	1219782	1221263	SPD_0310	0.0	UPF0371 protein SPD_0310	92.11	100.00	ABJ54480
DPGBHBGB_1	755241	757487	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
DPGBHBGB_1	65532	66524	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
DPGBHBGB_1	28544	29515	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
DPGBHBGB_1	1377417	1376266	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
DPGBHBGB_1	1340764	1338776	uvrB	0.0	excinuclease ABC, B subunit	88.99	100.00	AE014259_2
DPGBHBGB_1	1133676	1131946	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	88.04	100.00	AAK34199
DPGBHBGB_1	1816960	1815482	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.42	100.00	ADE32434
DPGBHBGB_1	606443	607744	Eno	0.0	enolase	86.21	99.77	ABP90468
DPGBHBGB_1	1801770	1799878	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	86.05	98.90	ABP91129
DPGBHBGB_1	760412	758703	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
DPGBHBGB_1	1654992	1656002	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
DPGBHBGB_1	907268	905907	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
DPGBHBGB_1	1705258	1703366	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
DPGBHBGB_1	592007	592897	bglB	5.18e-178	putative phospho--glucosidase	88.55	99.66	AAK33487
DPGBHBGB_1	822707	821772	SP_0676	7.29e-168	transcription facot	81.73	96.89	AAK74821
DPGBHBGB_1	757679	758536	metF	8.53e-165	methionine biosynthesis enzymes	86.36	99.31	AAK74739
DPGBHBGB_1	1027066	1026362	covR	4.32e-131	responder protein	85.53	100.00	AAK71319
DPGBHBGB_1	1200813	1200142	rr01	2.96e-124	response regulator	83.93	99.56	CAB54566
DPGBHBGB_1	1027066	1026362	walR	1.82e-121	DNA-binding response regulator	82.13	100.00	ANC99504
DPGBHBGB_1	764366	765034	CiaR	9.28e-118	two-component response regulator	84.75	99.55	AAK74935
DPGBHBGB_1	298339	299025	CovR	9.92e-118	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
DPGBHBGB_1	764366	765037	ciaR	1.01e-115	two component system response regulator	82.59	99.12	AIK75708
DPGBHBGB_1	66836	67231	SpxA2	3.65e-74	transcriptional regulator	90.91	100.00	ERL21461
DPGBHBGB_1	918626	919027	SpxA1	3.71e-66	transcriptional regulator	80.60	100.00	ERL19273
DPGBHBGB_1	146785	147264	RopE (M5005_Spy_1611)	2.47e-70	probable DNA-directed RNA polymerase subunit delta	80.62	83.77	AAK34603
DPGBHBGB_1	1491210	1493507	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
DPGBHBGB_1	1690854	1689835	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	95.88	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
DPGBHBGB_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found