safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	21	28	35.00

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
EBPEGGEH_1	1032774	1033564	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	100.00	100.00	-
EBPEGGEH_1	1290974	1291825	rfbD	0.0	Capsule	Immune modulation	100.00	100.00	WP_014621820
EBPEGGEH_1	1112943	1113704	srtA	0.0	Sortase A	Exoenzyme	100.00	100.00	-
EBPEGGEH_1	1077585	1078178	STR_RS05880	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
EBPEGGEH_1	1280523	1282307	STU_RS16230	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226325
EBPEGGEH_1	1282304	1283359	STU_RS16235	0.0	Capsule	Immune modulation	100.00	100.00	WP_002948022
EBPEGGEH_1	1283379	1284584	STU_RS16240	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226326
EBPEGGEH_1	1284584	1285393	STU_RS16245	0.0	Capsule	Immune modulation	100.00	100.00	WP_002948020
EBPEGGEH_1	1285377	1286333	STU_RS16250	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226328
EBPEGGEH_1	1286330	1287478	STU_RS16255	0.0	Capsule	Immune modulation	100.00	100.00	WP_041828290
EBPEGGEH_1	1287587	1288597	STU_RS16260	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226330
EBPEGGEH_1	1288597	1289871	STU_RS16265	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226331
EBPEGGEH_1	1077585	1078178	STER_RS06030	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
EBPEGGEH_1	1077585	1078178	STU_RS15260	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
EBPEGGEH_1	44880	45719	cbpD	0.0	Autolysin	Exoenzyme	99.64	100.00	WP_011226733
EBPEGGEH_1	859454	861112	fbp54	0.0	FBPs	Adherence	99.58	100.00	WP_011227179
EBPEGGEH_1	966943	968397	STU_RS14650	0.0	Capsule	Immune modulation	99.45	100.00	WP_011226049
EBPEGGEH_1	1078178	1079047	rfbA	0.0	Capsule	Immune modulation	99.19	100.00	WP_011681286
EBPEGGEH_1	966943	968397	STR_RS05265	0.0	Capsule	Immune modulation	98.35	100.00	WP_041827043
EBPEGGEH_1	966943	968397	STER_RS05320	0.0	Capsule	Immune modulation	97.06	99.79	WP_011681177
EBPEGGEH_1	594822	596125	eno	0.0	Streptococcal enolase	Exoenzyme	88.09	99.54	WP_000022832
EBPEGGEH_1	1577594	1578604	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.92	99.51	WP_002986042
EBPEGGEH_1	1076110	1077156	rfbB	0.0	Capsule	Immune modulation	80.61	100.00	WP_009659423
EBPEGGEH_1	144469	145752	tig/ropA	0.0	Trigger factor	Stress survival	80.58	99.77	WP_000107753
EBPEGGEH_1	962972	963555	SPD_RS01775	1.05e-146	Capsule	Immune modulation	83.08	96.20	WP_001819836
EBPEGGEH_1	966478	966942	STU_RS14645	0.0	Capsule	Immune modulation	100.00	63.52	WP_011226048
EBPEGGEH_1	966478	966942	STR_RS05260	0.0	Capsule	Immune modulation	98.50	63.52	WP_011227248
EBPEGGEH_1	966478	966942	STER_RS05315	0.0	Capsule	Immune modulation	97.63	63.52	WP_011681176

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
EBPEGGEH_1	1116391	1117374	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
EBPEGGEH_1	704908	705981	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
EBPEGGEH_1	512585	513553	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
EBPEGGEH_1	1455020	1457323	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.84	99.22	AAK74619
EBPEGGEH_1	733587	735833	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
EBPEGGEH_1	1195847	1197328	SPD_0310	0.0	UPF0371 protein SPD_0310	91.90	100.00	ABJ54480
EBPEGGEH_1	65755	66747	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
EBPEGGEH_1	28396	29367	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
EBPEGGEH_1	1341280	1340129	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.06	99.74	AAL00050
EBPEGGEH_1	1306807	1304825	uvrB	0.0	excinuclease ABC, B subunit	88.96	99.70	AE014259_2
EBPEGGEH_1	1100196	1098466	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.87	100.00	AAK34199
EBPEGGEH_1	1771339	1769861	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.42	100.00	ADE32434
EBPEGGEH_1	594822	596123	Eno	0.0	enolase	86.21	99.77	ABP90468
EBPEGGEH_1	1756603	1754711	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.89	98.90	ABP91129
EBPEGGEH_1	738758	737049	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
EBPEGGEH_1	1620535	1621545	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
EBPEGGEH_1	886682	885321	mnmE	0.0	central tRNA-modifying GTPase	82.02	99.56	ABP90420
EBPEGGEH_1	1672553	1670661	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
EBPEGGEH_1	580392	581282	bglB	1.28e-178	putative phospho--glucosidase	88.89	99.66	AAK33487
EBPEGGEH_1	798147	797212	SP_0676	7.11e-168	transcription facot	81.73	96.89	AAK74821
EBPEGGEH_1	736025	736882	metF	8.32e-165	methionine biosynthesis enzymes	86.36	99.31	AAK74739
EBPEGGEH_1	1005095	1004391	covR	3.37e-130	responder protein	84.68	100.00	AAK71319
EBPEGGEH_1	1169575	1168904	rr01	1.15e-125	response regulator	84.38	99.56	CAB54566
EBPEGGEH_1	1005095	1004391	walR	2.42e-120	DNA-binding response regulator	80.85	100.00	ANC99504
EBPEGGEH_1	302110	302796	CovR	9.68e-118	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
EBPEGGEH_1	742711	743361	CiaR	1.33e-116	two-component response regulator	86.17	96.88	AAK74935
EBPEGGEH_1	742711	743361	ciaR	6.86e-114	two component system response regulator	83.87	96.02	AIK75708
EBPEGGEH_1	67044	67439	SpxA2	3.56e-74	transcriptional regulator	90.91	100.00	ERL21461
EBPEGGEH_1	145910	146410	RopE (M5005_Spy_1611)	9.51e-75	probable DNA-directed RNA polymerase subunit delta	80.84	87.43	AAK34603
EBPEGGEH_1	1455020	1457317	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.81	98.08	MYN69560
EBPEGGEH_1	1658092	1657073	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.18	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
EBPEGGEH_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found