safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	21	28	35.00

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
GGJOBPCC_1	1037375	1038165	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	100.00	100.00	-
GGJOBPCC_1	1295580	1296431	rfbD	0.0	Capsule	Immune modulation	100.00	100.00	WP_014621820
GGJOBPCC_1	1082194	1082787	STU_RS15260	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
GGJOBPCC_1	1285129	1286913	STU_RS16230	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226325
GGJOBPCC_1	1287985	1289190	STU_RS16240	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226326
GGJOBPCC_1	1289190	1289999	STU_RS16245	0.0	Capsule	Immune modulation	100.00	100.00	WP_002948020
GGJOBPCC_1	1289983	1290939	STU_RS16250	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226328
GGJOBPCC_1	1290936	1292084	STU_RS16255	0.0	Capsule	Immune modulation	100.00	100.00	WP_041828290
GGJOBPCC_1	1292193	1293203	STU_RS16260	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226330
GGJOBPCC_1	1293203	1294477	STU_RS16265	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226331
GGJOBPCC_1	1286910	1287965	STU_RS16235	0.0	Capsule	Immune modulation	99.91	100.00	WP_002948022
GGJOBPCC_1	1117553	1118314	srtA	0.0	Sortase A	Exoenzyme	99.87	100.00	-
GGJOBPCC_1	971541	972995	STR_RS05265	0.0	Capsule	Immune modulation	99.86	100.00	WP_041827043
GGJOBPCC_1	1082194	1082787	STR_RS05880	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
GGJOBPCC_1	1082194	1082787	STER_RS06030	0.0	Capsule	Immune modulation	99.66	100.00	WP_011226149
GGJOBPCC_1	44766	45605	cbpD	0.0	Autolysin	Exoenzyme	99.64	100.00	WP_011226733
GGJOBPCC_1	863961	865619	fbp54	0.0	FBPs	Adherence	99.58	100.00	WP_011227179
GGJOBPCC_1	1082787	1083656	rfbA	0.0	Capsule	Immune modulation	99.54	100.00	WP_011681286
GGJOBPCC_1	971541	972995	STU_RS14650	0.0	Capsule	Immune modulation	97.94	100.00	WP_011226049
GGJOBPCC_1	971541	972995	STER_RS05320	0.0	Capsule	Immune modulation	96.92	99.79	WP_011681177
GGJOBPCC_1	600514	601817	eno	0.0	Streptococcal enolase	Exoenzyme	88.09	99.54	WP_000022832
GGJOBPCC_1	1582221	1583231	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.92	99.51	WP_002986042
GGJOBPCC_1	1080718	1081764	rfbB	0.0	Capsule	Immune modulation	80.71	100.00	WP_009659423
GGJOBPCC_1	150162	151445	tig/ropA	0.0	Trigger factor	Stress survival	80.58	99.77	WP_000107753
GGJOBPCC_1	967570	968153	SPD_RS01775	1.06e-146	Capsule	Immune modulation	83.08	96.20	WP_001819836
GGJOBPCC_1	971076	971540	STR_RS05260	0.0	Capsule	Immune modulation	99.78	63.52	WP_011227248
GGJOBPCC_1	971076	971540	STU_RS14645	0.0	Capsule	Immune modulation	98.28	63.52	WP_011226048
GGJOBPCC_1	971076	971540	STER_RS05315	0.0	Capsule	Immune modulation	97.20	63.52	WP_011681176

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
GGJOBPCC_1	1121001	1121984	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
GGJOBPCC_1	709429	710502	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
GGJOBPCC_1	518277	519245	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
GGJOBPCC_1	1459649	1461952	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
GGJOBPCC_1	738109	740355	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
GGJOBPCC_1	1200457	1201938	SPD_0310	0.0	UPF0371 protein SPD_0310	91.90	100.00	ABJ54480
GGJOBPCC_1	65641	66633	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
GGJOBPCC_1	28282	29253	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
GGJOBPCC_1	1345909	1344758	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.06	99.74	AAL00050
GGJOBPCC_1	1311413	1309431	uvrB	0.0	excinuclease ABC, B subunit	88.96	99.70	AE014259_2
GGJOBPCC_1	1104807	1103077	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.87	100.00	AAK34199
GGJOBPCC_1	1775965	1774487	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.42	100.00	ADE32434
GGJOBPCC_1	600514	601815	Eno	0.0	enolase	86.21	99.77	ABP90468
GGJOBPCC_1	1761229	1759337	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.89	98.90	ABP91129
GGJOBPCC_1	743280	741571	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
GGJOBPCC_1	1625162	1626172	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
GGJOBPCC_1	891189	889828	mnmE	0.0	central tRNA-modifying GTPase	82.02	99.56	ABP90420
GGJOBPCC_1	1677179	1675287	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
GGJOBPCC_1	586084	586974	bglB	1.28e-178	putative phospho--glucosidase	88.89	99.66	AAK33487
GGJOBPCC_1	802670	801735	SP_0676	7.13e-168	transcription facot	81.73	96.89	AAK74821
GGJOBPCC_1	740547	741404	metF	8.34e-165	methionine biosynthesis enzymes	86.36	99.31	AAK74739
GGJOBPCC_1	1009696	1008992	covR	3.45e-130	responder protein	84.68	100.00	AAK71319
GGJOBPCC_1	1174185	1173514	rr01	1.16e-125	response regulator	84.38	99.56	CAB54566
GGJOBPCC_1	1009696	1008992	walR	2.12e-120	DNA-binding response regulator	80.85	100.00	ANC99504
GGJOBPCC_1	307803	308489	CovR	9.71e-118	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
GGJOBPCC_1	747234	747902	CiaR	6.86e-117	two-component response regulator	83.86	99.55	AAK74935
GGJOBPCC_1	747234	747905	ciaR	6.81e-115	two component system response regulator	81.70	99.12	AIK75708
GGJOBPCC_1	66930	67325	SpxA2	3.57e-74	transcriptional regulator	90.91	100.00	ERL21461
GGJOBPCC_1	151603	152103	RopE (M5005_Spy_1611)	9.53e-75	probable DNA-directed RNA polymerase subunit delta	80.84	87.43	AAK34603
GGJOBPCC_1	1459649	1461946	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
GGJOBPCC_1	1662718	1661699	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.18	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
GGJOBPCC_2	1	948	rep37	950 / 948	94.74	AF177168

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
GGJOBPCC_1	0.0	1.0	0.0
GGJOBPCC_2	1.0	0.0	1.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found